18-07-2023 дата публикации
Номер: CN116448917A
Принадлежит:
The invention provides a method for detecting the biogenic amine level in oyster product preservation, and belongs to the technical field of marine product detection.The method comprises the steps that firstly, preserved oysters are taken, viscera of the oysters are removed, and the oysters are washed clean and then cut up; then taking 1-3g of the chopped oysters, adding 8-12mL of a perchloric acid solution, uniformly mashing by using a homogenizer, then putting the mixture into a centrifugal machine for centrifuging, taking supernate, adding 8-12mL of the perchloric acid solution into a precipitation solution, uniformly stirring, repeating centrifuging once, taking the supernate, mixing the supernate twice, adding the perchloric acid solution, and fixing the volume to 20-30mL to obtain an extracting solution; measuring 1mL of the extracting solution, putting the extracting solution into a 15mL plastic centrifugal tube, sequentially adding 50-150mu L of a sodium hydroxide solution, 250- ...
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