Antimicrobial materials

Method and apparatus for inactivating contaminants in biological fluid

Antimicrobial materials

Method for testing blood units for viral contamination

Method for testing blood units for viral contamination

Methods and apparatus for inactivating contaminants in biological fluid

Methods and apparatus for inactivating contaminants in biological fluid

Centrifuge and separation vessel therefore

The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation.

Method for separating a sample into density specific fractions

The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation. 1. A method for separation of a sample into at least two fractions of different densities , the method including the steps of:identifying a centrifuge vessel having an outer wall surrounding a fluid containing interior space of the vessel, a barrier inside the outer wall, the barrier partially dividing the interior space into at least two regions, the barrier having a lip with the two regions connected above the lip and the two regions spaced apart below the lip;placing a sample into the interior space of the vessel;positioning the vessel into a centrifuge;spinning the vessel within the centrifuge to separate the sample into differing density fractions; andstopping said spinning step while keeping the separated differing density fractions at least partially divided by the barrier into the at least two regions within the centrifuge vessel.2. The method of wherein said identifying a centrifuge vessel step includes locating the lip of the barrier between a higher gee side and a lower gee side of the vessel at a particular fixed position at least partially correlated with expected prevalence of the at least two fractions of the sample.3. The ...

Apparatus and method for separating and isolating components of a biological fluid

A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.

Apparatus and method for separating and isolating components of a biological fluid

A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber. 1. A device for separating and isolating components of a biological fluid with centrifugation , said device comprising:an enclosure for containing a biological fluid having multiple components;a float slideably disposed within said enclosure and having an interior isolation chamber;said float having a first aperture means for allowing a flow of the biological fluid into said interior isolation chamber of said float as a function of a first pressure differential of biological fluid on the biological fluid above and below said first aperture means; andsaid float having a second aperture means for allowing the flow of the biological fluid out of said interior isolation chamber of said float as a function of a second pressure differential of biological fluid on the biological fluid above and below said second aperture means.2. The device of further including a third aperture means for allowing a flow of the biological fluid out of said interior isolation chamber of said float as a function of a third pressure differential of biological fluid on ...

Stem and progenitor cell compositions recovered from bone marrow or cord blood; system and method for preparation thereof

The invention includes compositions of stem and progenitor cells recovered from bone marrow or cord blood containing most of the viable CD34+ cells and substantially depleted of red blood cells resident in the original sample, without any xenobiotic additives to aid cell separation. The invention also includes a system and method for preparing the compositions. The system includes a bag set and a processing device, which utilizes an optical sensor, microcontroller, servo motor, accelerometer, load cell, and battery. The system and method utilize centrifugation to stratify the cells into layers and then separate and transfer the stem cells into a stem cell bag. The processing device's microcontroller receives input from the device's accelerometer, load cell and optical sensor to direct the metering valve in the bag set to open and close to permit the transfer of as many stems cells as possible with as few red cells as possible. 1. A composition of stem cells prepared from anticoagulated bone marrow , comprising:plasma;RBCs;WBCs; andviable stem cells;wherein said composition does not include a xenobiotic additive;wherein said stem cells include CD34+ cells; and furtherwherein the ratio of said RBCs to said CD34+ cells in said composition is less than about 2000 to 1.2. A composition of stem cells prepared from anticoagulated bone marrow , comprising:plasma;RBCs;WBCs; andviable stem cells;wherein said composition does not include a xenobiotic additive;wherein said stem cells include ALDH Br+ cells; andwherein the ratio of said RBCs to said ALDH Br+ cells in said composition is less than about 2000 to 1.3. A composition of stem cells prepared from anticoagulated cord blood , comprising:plasma;RBCs;WBCs; andviable stem cells;wherein said composition does not include a xenobiotic additive;wherein said stem cells include CD34+ cells; andwherein the ratio of said RBCs to said CD34+ cells in said composition is less than about 15,000 to 1.4. A composition of stem cells ...

Apparatus and method for separating and isolating components of a biological fluid

A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.

Method of Preconditioning of Cell Suspensions

An apparatus for the preparation of cells for implantation into a living body is disclosed. The apparatus comprises a vessel substantially impermeable to gaseous oxygen; and fluid within said vessel, the fluid having a maximal dissolved oxygen capacity substantially equivalent to normal saline yet having a hypoxic oxygen concentration between about 0% to about 5% of said maximal dissolved oxygen capacity. The bag oxygen concentration level remains low when the apparatus is stored at 22°-25° C. in normal atmospheric conditions for a period of at least 30 days. The present invention simplifies the process of achieving donor cell hypoxic preconditioning for cell implantation, and may be used to bathe said cells to be transplanted for a sufficient time to activate the hypoxic metabolic pathway.

Centrifuge separation method and apparatus using a medium density fluid

The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation. A medium density fluid is used within the vessel to further isolate constituents of differing densities. 1. A method for separating a higher density constituent in an initial fluid sample from other portions of the sample having a lower density , the method including the steps of:identifying an enclosure;placing a sample into the enclosure;the sample of said placing step including a higher density constituent and at least one other portion of the sample having a lower density than said higher density constituent;locating at least one medium density fluid in the enclosure;the medium density fluid of said locating step having a density between the density of the higher density constituent and the density of the at least one other portion;centrifuging the enclosure after said placing step and said locating step, until the higher density constituent is spaced from the at least one other portion of the sample by the medium density fluid; andwherein said identifying an enclosure step includes the enclosure having an outer wall surrounding a fluid containing interior space of the enclosure, a barrier inside the outer wall, the barrier partially ...

APPARATUS FOR CENTRIFUGATION AND METHODS THEREFORE

A centrifuge vessel is configured to have at least two chambers therein separated by a divider, and with a spillway around a lip at an end of the divider to join the chambers. After centrifugation differing density phases of an original sample remain on opposite sides of the divider. Separate extraction ports are provided for removal of differing density phases of the original sample from opposite sides of the divider. A method of use of the centrifuge vessel includes centrifugation and extraction at differing orientations of the vessel for convenient and reliable extraction of differing density fractions from the original sample. 1: A centrifuge vessel for separation of a heterogeneous fluid into a plurality of separate constituents having different densities , the vessel comprising in combination:a housing including a floor and side walls extending up from said floor;at least two chambers including a lower chamber and an upper chamber, said lower chamber closer to said floor than said upper chamber;a divider wall interposed between said upper chamber and said lower chamber;a spillway around said divider wall, said spillway joining said upper chamber to said lower chamber; andan outlet port extending from said lower chamber and out of said housing, such that a constituent of the fluid sample within said lower chamber can be removed from said lower chamber.2: The vessel of wherein said housing includes an inlet passing thereinto claim 1 , said inlet adapted to allow a sample fluid to be passed into said housing.3: The vessel of wherein a suction port is provided into said housing separate from said inlet claim 2 , such that a source of vacuum can be coupled to said suction port and gases within said housing can be removed as a fluid sample is passed into said housing through said inlet.4: The vessel of wherein a second outlet port is provided extending between said upper chamber and a space outside of said housing.5: The vessel of wherein said divider wall has a ...

Apparatus and Method for Separating and Isolating Components of a Biological Fluid

A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for dosing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.

Apparatus for Preconditioning of Cell Suspensions

An apparatus for the preparation of cells for implantation into a living body is disclosed. The apparatus comprises a vessel substantially impermeable to gaseous oxygen; and fluid within said vessel, the fluid having a maximal dissolved oxygen capacity substantially equivalent to normal saline yet having a hypoxic oxygen concentration between about 0% to about 5% of said maximal dissolved oxygen capacity. The bag oxygen concentration level remains low when the apparatus is stored at 22°-25° C. in normal atmospheric conditions for a period of at least 30 days. The present invention simplifies the process of achieving donor cell hypoxic preconditioning for cell implantation, and may be used to bathe said cells to be transplanted for a sufficient time to activate the hypoxic metabolic pathway. 1. A method for the preparation of mammalian cells for implantation into a living body , the method comprising the steps of: i. a first vessel substantially impermeable to gaseous oxygen;', 'ii. hypoxic fluid within said first vessel, the hypoxic fluid having a maximal dissolved oxygen capacity and having an oxygen concentration level of not more than 5% of said maximal dissolved oxygen capacity; and', 'iii. a mammalian cell retaining chamber comprising a filter and mammalian cells having a level of HIF-1α; and, 'a. providing an apparatus comprisingb. contacting said hypoxic fluid with said mammalian cells within said filter.2. The method of wherein said oxygen concentration level is between 0.05% and 1.5% of said maximal dissolved oxygen capacity.3. The method of wherein said hypoxic fluid within said first vessel maintains said oxygen concentration level when said first vessel is stored at 22°-25° C. in normal atmospheric conditions for a period of at least 30 days.4. The method of further comprising storing said first vessel at 22°-25° C. in normal atmospheric conditions for a period of at least 30 days such that said oxygen concentration of not more than 5% of said maximal ...

METHOD AND APPARATUS FOR PREPARING SINGLE DONOR THROMBIN SERUM

A method for preparing thrombin serum, the method comprising obtaining blood fluid sample, contacting a first aliquot of the blood fluid with a procoagulant agent to form prothrombinase enzyme complex bound to the surface of the procoagulant agent so as to obtain an activated procoagulant agent that may be stored. The activated procoagulant agent may then be contacted with a second aliquot of the blood fluid containing prothrombin so as to obtain thrombin serum, which may be extracted and contacted with fibrinogen to obtain fibrin. 1. A method for preparing thrombin serum , the method comprising the steps of:obtaining an activated procoagulant agent; andcontacting said activated procoagulant agent with a blood fluid aliquot to form a mixture comprising thrombin serum; andextracting said thrombin serum from said mixture.2. A method for preparing thrombin serum , the method comprising the steps of:contacting a first blood fluid aliquot with a procoagulant agent to form an activated procoagulant agent;storing said activated procoagulant agent;contacting a second blood fluid aliquot with said stored activated procoagulant agent to form a mixture comprising thrombin serum; andextracting said thrombin serum from said mixture.3. A method for preparing thrombin serum , the method comprising the steps of:contacting a first blood fluid aliquot with a procoagulant agent to form an activated procoagulant agent comprising a prothrombinase enzyme complex bound to the surface of the procoagulant agent;storing said activated procoagulant agent;contacting said stored activated procoagulant agent with a second aliquot of blood fluid to form a mixture comprising fibrin gel;triturating said fibrin gel;after said triturating step, extracting thrombin serum from said mixture; andcontacting said extracted thrombin serum with fibrinogen to obtain fibrin.4. A method for preparing ethanol stabilized thrombin serum , the method comprising the steps of:contacting a first blood fluid aliquot ...

COLLAPSIBLE CENTRIFUGATION VIAL SYSTEM AND METHOD

A vial and system are configured to simplify fluid input and extraction therefrom. The vial has a first end opposite a second end and with a collapsible sidewall. The sidewall can be collapsed, such as by imparting a differential pressure between an interior and exterior of the vial, for simplified fluid extraction and smooth extraction without disturbing boundaries between separated components. The vial typically has a bulb at a first end and a neck extending away from the bulb and toward the second end. An inlet spaced from the first end can be configured to attach to various different interfaces or processing elements for input and output of fluids. A support cup can be provided for use with the vial during centrifugation to allow the vial to withstand any centrifugation forces. Various fluid processing methodologies are supported by the collapsible vial, especially including methods of separation of components of blood. 1. A method for separation of components of a fluid mixture , the method including the steps of:loading a vial with the fluid mixture, the vial having a first end opposite a second end, and with a collapsible sidewall extending at least partially between the first end and the second end, and with an inlet spaced from the first end;rotating the vial in a centrifuge about a rotational axis, with the first end further from the rotational axis than the second end, until the fluid mixture is at least partially separated into at least two components, including a first component and a second component, the first component closer to the first end of the vial than the second component; andejecting at least a portion of the second component of the fluid mixture from the vial through the inlet by collapsing the sidewall of the vial to reduce vial volume.2. The method of wherein said loading step is preceded by collapsing the vial to have a lesser volume claim 1 , with said vial increasing in volume as said loading step occurs.3. The method of wherein said ...

Methods and apparatus for inactivating contaminants in biological fluid

Apparatus and methods for treating a biological fluid with light and for inactivating contaminants in biological fluid. The biological fluid is contacted with a light source providing a high intensity light to the biological fluid. The biological fluid may include a quantity of a photochemical agent that when activated by light is operable to cause inactivation of at least some of the contaminants.

Method and apparatus for inactivating contaminants in biological fluid

Apparatus and methods for treating a biological fluid with light and for inactivating contaminants in biological fluid. The biological fluid is contacted with a light source providing a high intensity light to the biological fluid. The biological fluid may include a quantity of a photochemical agent that when activated by light is operable to cause inactivation of at least some of the contaminants.

Method and apparatus for preparing single donor thrombin serum

A method for preparing thrombin serum, the method comprising obtaining blood fluid sample, contacting a first aliquot of the blood fluid with a procoagulant agent to form prothrombinase enzyme complex bound to the surface of the procoagulant agent so as to obtain an activated procoagulant agent that may be stored. The activated procoagulant agent may then be contacted with a second aliquot of the blood fluid containing prothrombin so as to obtain thrombin serum, which may be extracted and contacted with fibrinogen to obtain fibrin.

Rapid-action, anti-jolt edger charger system

A lumber workpiece charger system for an edger wherein transfer of a workpiece occurs under rapid non-jolting control for precise positioning, with the workpiece, throughout its delivery, clamped in proper orientation for hand-off to the edger.

Methods and apparatus for inactivating contaminants in biological fluid

Centrifuge and separation vessel therefore

Antimicrobial materials

An antimicrobial material incorporated with a molecular halogen is provided. The antimicrobial material includes a plastic material and a molecular halogen entrapped within the plastic material. A directional release material is also provided in the present application. The directional release material allows for the control of the rate and sire of halogen release. Still further, method of impregnating a plastic material is also provided.

Method and apparatus for inactivating contaminants in biological fluid

ANTIMICROBIAL MATERIALS

Antimicrobial materials

An antimicrobial material incorporated with a molecular halogen is provided. The antimicrobial material includes a plastic material and a molecular halogen entrapped within the plastic material. A directional release material is also provided in the present application. The directional release material allows for the control of the rate and site of halogen release. Still further, method of impregnating a plastic material are also provided.

Expression of ocean fish antifreeze peptide in a food grade organism and its application in food products

A method is described for producing an AFP type III HPLC 12 polypeptide in yeast.

Antimicrobial materials

An antimicrobial material incorporated with a molecular halogen is provided. The antimicrobial material includes a plastic material and a molecular halogen entrapped within the plastic material. A directional release material is also provided in the present application. The directional release material allows for the control of the rate and site of halogen release. Still further, method of impregnating a plastic material is also provided.

Method and apparatus for preparing single donor thrombin serum

Apparatus for centrifugation and methods therefore

A centrifuge vessel is configured to have at least two chambers therein separated by a divider, and with a spillway around a lip at an end of the divider to join the chambers. After centrifugation differing density phases of an original sample remain on opposite sides of the divider. Separate extraction ports are provided for removal of differing density phases of the original sample from opposite sides of the divider. A method of use of the centrifuge vessel includes centrifugation and extraction at differing orientations of the vessel for convenient and reliable extraction of differing density fractions from the original sample.

Method and apparatus for inactivating contaminants in biological fluid

Apparatus and methods for treating a biological fluid with light and for inactivating contaminants in biological fluid. The biological fluid is contacted with a light source (24) providing a high intensity light to the biological fluid. The biological flu id may include a quantity of a photochemical agent that, when activated by light, is operab le to cause inactivation of at least some of the contaminants. The apparatus includes a light box (10), including a retractable shutter assembly (26) that encloses the light source (24). The shutter assembly (26) includes a cylindrical tube (41) that, when raised, shields th e light source (24). In addition, cylindrical tube (28), attached to platform (36) of base assemb ly (22), encloses the light source (24) and the shutter assembly (26).

Rapid-action, anti-jolt edger charger system

A lumber workpiece charger system for an edger wherein transfer of a workpiece occurs under rapid non-jolting control for pieces positioning, with the workpiece, throughout its delivery, clamped in proper orientation for hand-off to the edger.

Apparatus for centrifugation and methods therefore

A centrifuge vessel is configured to have at least two chambers therein separated by a divider, and with a spillway around a lip at an end of the divider to join the chambers. After centrifugation differing density phases of an original sample remain on opposite sides of the divider. Separate extraction ports are provided for removal of differing density phases of the original sample from opposite sides of the divider. A method of use of the centrifuge vessel includes centrifugation and extraction at differing orientations of the vessel for convenient and reliable extraction of differing density fractions from the original sample.

Adipose tissue graft for wound healing

. An improved method for preparing an adipose tissue biocomposite graft to serve a wide range of medical applications is presented. In particular, the embodiments consider a performing lipoplasty to derive a plurality of adipose tissue fragments containing at least one viable stem cell from a donor, harvesting said plurality of adipose tissue fragments from said donor, placing said plurality of adipose tissue fragments in contact with appropriate concentrations of a thrombin source and a fibrinogen source to achieve an appropriate gelling reaction and applying the mixture of said adipose tissue fragments, said thrombin source and said fibrinogen source to a wound site of the donor so as to promote wound healing. The adipose biocomposite graft of the present invention can be easily processed, molded and customized to precise dimensions. The present invention employs a three dimensional mold cavity to prepare multiple castings of said adipose tissue biocomposite grafts for an individual donor.

Method and apparatus for preparing single donor thrombin serum

A method for preparing thrombin serum, the method comprising obtaining blood fluid sample, contacting a first aliquot of the blood fluid with a procoagulant agent to form prothrombinase enzyme complex bound to the surface of the procoagulant agent so as to obtain an activated procoagulant agent that may be stored. The activated procoagulant agent may then be contacted with a second aliquot of the blood fluid containing prothrombin so as to obtain thrombin serum, which may be extracted and contacted with fibrinogen to obtain fibrin.

Antimikrobielle materialen

Vorrichtung zur inaktivierung von verunreinigungen in biologischen flüssigkeiten

Verfahren zur testung von blutkonserven auf virale verunreinigung

Improvments on carriage tops

Method and apparatus for preparing single donor thrombin serum

A method for preparing thrombin serum, the method comprising obtaining blood fluid sample, contacting a first aliquot of the blood fluid with a procoagulant agent to form prothrombinase enzyme complex bound to the surface of the procoagulant agent so as to obtain an activated procoagulant agent that may be stored. The activated procoagulant agent may then be contacted with a second aliquot of the blood fluid containing prothrombin so as to obtain thrombin serum, which may be extracted and contacted with fibrinogen to obtain fibrin.

Apparatus for centrifugation and methods therefore

A centrifuge vessel is configured to have at least two chambers therein separated by a divider, and with a spillway around a lip at an end of the divider to join the chambers. After centrifugation differing density phases of an original sample remain on opposite sides of the divider. Separate extraction ports are provided for removal of differing density phases of the original sample from opposite sides of the divider. A method of use of the centrifuge vessel includes centrifugation and extraction at differing orientations of the vessel for convenient and reliable extraction of differing density fractions from the original sample.

Apparatus and method for separating and isolating components of a biological fluid

A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for dosing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.