Hematology systems and methods

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems.

Hematology systems and methods

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Parrticles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems.

HEMATOLOGY SYSTEMS AND METHODS

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Parrticles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems. 15-. (canceled)6. A method for imaging particles using a particle analysis system configured for combined viscosity and geometric hydrofocusing , the particles included within a blood fluid sample , the method comprising:injecting a sheath fluid along a flowpath of a flowcell of the particle analyzer, the sheath fluid having a viscosity that is different from a viscosity of the blood fluid sample;injecting the blood fluid sample from a sample fluid injection tube at a flow rate into the flowing sheath fluid within the flowcell so as to provide a sample fluid stream having a first thickness adjacent the injection tube, the flowpath of the flowcell having a decrease in flowpath size such that thickness of the sample fluid stream decreases from the initial thickness to a second thickness adjacent an image capture site; andimaging a first plurality of the particles from the sample at the image capture site of the flowcell;wherein the decrease in flowpath size is defined by a proximal flowpath transition zone portion having a proximal thickness, and distal flowpath transition zone portion ...

Method and composition for staining and processing a urine sample

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300.

Method and composition for staining and processing a urine sample

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300.

Method and composition for staining and sample processing

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents, one or more permeabilizing agents, and one or more fixing agents. The particle contrast agent composition can include Crystal Violet, New Methylene Blue, Saponin, and Gluteraldehyde.

METHOD AND COMPOSITION FOR STAINING AND PROCESSING A URINE SAMPLE

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300.

Hematology systems and methods

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems.

METHOD AND COMPOSITION FOR STAINING AND SAMPLE PROCESSING

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents, one or more permeabilizing agents, and one or more fixing agents. The particle contrast agent composition can include Crystal Violet, New Methylene Blue, Saponin, and Gluteraldehyde. 1. A particle contrast agent composition for staining a blood fluid sample being imaged in an automated particle analysis system comprising:at least one particle contrast agent selected from the group consisting of Crystal Violet, New Methylene Blue, Methyl Green, Eosin Y, and Safranin O;a permeabilizing agent selected from the group consisting of a surfactant, a saponin, a quarternary ammonium salt, a nonionic surfactant, a detergent; and a zwitterionic surfactant; anda fixing agent selected from the group consisting of gluteraldehyde and formaldehyde.2. The composition of claim 1 , wherein:the permeabilizing agent is saponin present in amounts sufficient to result in concentrations between about 50 mg/L and about 750 mg/L under staining conditions; andthe fixing agent is gluteraldehyde present in amounts sufficient to result in concentrations at or below 0.1% under staining conditions.3. The composition of claim 2 , wherein:the at least one particle contrast agent includes Crystal Violet, New Methylene Blue, and Eosin-Y;a ratio of the Crystal Violet to the New Methylene Blue is between about 1:90 to about 1:110 under staining conditions; andthe Eosin-Y is present in amounts sufficient to result in concentrations of about 3 μM to about 300 μM under staining conditions.4. The composition of claim 3 , wherein:the Crystal Violet is present in amounts sufficient to result in concentrations of about 6 μM to about 10 μM under staining conditions;the New Methylene Blue is present in amounts sufficient to result in ...

METHOD AND COMPOSITION FOR STAINING AND PROCESSING A URINE SAMPLE

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300. 1. A particle contrast agent composition for staining particles of a urological fluid sample for imaging in an automated particle analysis system comprising:Crystal Violet present in amounts sufficient to result in concentrations between 50 μM and 500 μM under staining conditions; anda permeabilizing agent selected from the group consisting of 5 PD Lytic and saponin.2. The composition of claim 1 , wherein:the permeabilizing agent is 5 PD Lytic present in amounts sufficient to result in concentrations of about 3.5% by weight under staining conditions.3. The composition of claim 2 , wherein:the Crystal Violet is present in amounts sufficient to result in concentrations of about 86 μM under staining conditions.4. The composition of claim 3 , additionally comprising:a phosphate buffered saline including at least sodium phosphate dibasic and potassium phosphate monobasic.5. The composition of claim 1 , additionally comprising an antimicrobial agent.6. The composition of claim 5 , wherein:the Crystal Violet is present in amounts sufficient to result in concentrations of about 86 μM under staining conditions;the Crystal Violet is approximately 80% pure or greater; andthe antimicrobial agent is Proclin 300.7. The composition of claim 6 , additionally including:sodium chloride; anda phosphate buffered saline including at least sodium phosphate dibasic and potassium phosphate monobasic.8. A method of treating particles of a urological fluid sample for imaging using in an automated particle analysis system ...

method and composition for staining and processing a urine sample

MÉTODO E COMPOSIÇÃO PARA COLORAÇÃO E PROCESSAMENTO DE UMA AMOSTRA DE URINA. A presente descrição refere-se a uma metodologia de coloração que emprega uma composição de agente de contraste de partícula capaz de rapidamente colorir células em uma etapa única. A composição de agente de contraste de partícula pode ser compreendida de uma combinação de um ou mais agentes de contraste de partícula e um ou mais agentes permeabilizantes, opcionalmente incluindo um ou mais agentes fixadores e outros componentes. A composição de agente de contraste de partícula pode incluir Cristal Violeta, 5PD-Lytic, e Proclin 300. METHOD AND COMPOSITION FOR COLORING AND PROCESSING A URINE SAMPLE. The present description relates to a staining methodology that employs a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300.

Hematology systems and methods

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems.

Hematology systems and methods