20-03-2006 дата публикации
Номер: AP0000001592A
Автор:
GRONOWITZ SIMON [SE],
KALLANDER CLAS [SE],
MALMSTEN ANDERS [SE],
GRONOWITZ SIMON,
KALLANDER CLAS,
MALMSTEN ANDERS,
SHAO XINGWU,
GRONOWITZ Simon,
KALLANDER Clas,
MALMSTEN Anders,
SHAO Xingwu
Принадлежит:
A method of testing phenotypic drug susceptibility in an enveloped virus-infected mammalian individual by testing on an enzyme packaged into an enveloped virus, such as HIV, recovered from a biological sample, such as blood or plasma, from said individual is described. The method comprises the steps of a) adding an enzyme inactivating agent to the sample for inactivating polymerase activity other than that present in the envelope virion, b) removing the enzyme inactivating agent, enzyme activity blocking antibodies, endogenous enzyme activity inhibitors and antiviral drugs, c) lysing the virus particle to release the enzyme, d) recovering the concentrated purified viral enzyme, such as a HIV reverse transcriptase (RT), resulting from c) and determining the drug sensitivity profile of the individual from the recovered enzyme by using sensitive enzyme assays. The drug sensitivity profile may be used for selecting drug treatment therapy. A commercial package is included.
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