MELON PLANTS WITH ENHANCED FRUIT YIELDS

A plant or a part thereof, the plant bearing more than 12 fruit, the fruit being seedless. Methods of generating same and breeding same are also disclosed. 1Cucumis melo. A plant or a part thereof , the plant bearing more than 12 fruit , said fruit being seedless.2Cucumis melo. A plant having a MELO3C009603/melo3c009603 genome such that upon self-pollination , 25% of F1 bear more than 12 fruit , said fruit being seedless.3. The plant of claim 1 , bearing more than 15 fruit.4. The plant of claim 1 , bearing more than 20 fruit.5Cucumis melo. The plant of claim 1 , having a similar total soluble solid (TSS) content and β-carotene content as a wild-type plant.6Cucumis melo. The plant of claim 1 , wherein the weight of total fruit of said plant is greater than the weight of total fruit of a wild-type plant.7C. melo. The plant of claim 1 , being of a variety Cantalupensis.8. The plant of claim 1 , wherein both alleles of MELO3C009603 of the genome of the plant have a loss of function mutation that results in a seedless trait.9. The plant of claim 8 , wherein both alleles of said MELO3C009603 have an F/I mutation at position 97 thereof.10. The plant of claim 8 , wherein the polynucleotide sequence of said MELO3C009603 is as set forth in SEQ ID NO: 7.11. The plant of claim 1 , wherein the polypeptide sequence of MELO3C009603 is as set forth in SEQ ID NO: 8.12C. melo. A cutting of a plant of the plant of .13. The plant part of claim 1 , being selected from the group consisting of roots claim 1 , stems claim 1 , leaves claim 1 , cotyledons claim 1 , flowers claim 1 , fruit claim 1 , embryos and pollen.14. A seed of the plant of .15. A cell having the genome of the plant of .16. A culture comprising a plurality of the cells of .17. The plant part of claim 1 , selected from the group consisting of roots claim 1 , stems claim 1 , leaves claim 1 , cotyledons claim 1 , flowers claim 1 , fruit claim 1 , embryos and pollen.18C. meloC. meloC. melo.. A method of breeding a first ...

METHODS OF PRODUCING MOGROSIDES AND COMPOSITIONS COMPRISING SAME AND USES THEREOF

Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT). Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol. 123-. (canceled)24. A method of synthesizing a mogrol or mogrol precursor product from a mogrol precursor substrate , the method comprising contacting at least one mogrol precursor substrate with a mogroside pathway enzyme , wherein:(a) when said mogrol precursor product comprises diepoxy squalene and said mogrol precursor substrate comprises squalene or oxidosqualene, said mogroside pathway enzyme comprises a squalene epoxidase polypeptide at least 94% identical to SEQ ID NO: 14 or 89% identical to SEQ ID NO: 16, wherein said polypeptide catalyzes diepoxysqualene synthesis from squalene or oxidosqualene, thereby producing diepoxy squalene,(b) when said mogrol precursor product comprises 3 hydroxy, 24-25 epoxy cucurbitadienol and said mogrol precursor substrate comprises diepoxy squalene, said mogrol pathway enzyme comprises a cucurbitadienol synthetase polypeptide at least 60% ...

ISOLATED POLYNUCLEOTIDES AND METHODS AND PLANTS USING SAME FOR REGULATING PLANT ACIDITY

Provided are isolated polynucleotides comprising a nucleic acid sequence encoding a polypeptide, wherein the polypeptide modulates acidity of a plant. Also provided are nucleic acid constructs and plant cells comprising same and methods of using same for modulating acidity of a plant. 1. An isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide having at least 60% identity to a polypeptide selected from the group consisting of SEQ ID NO:2 , 4 , 5 , 6 , 8 , 9 10 , 28 and 29 , wherein said polypeptide modulates acidity of a plant.2. The isolated polynucleotide of claim 1 , wherein said polynucleotide is at least 60% identical to a polynucleotide selected from the group consisting of SEQ ID NOs: 1 claim 1 , 3 claim 1 , 30 claim 1 , 31 claim 1 , 32 claim 1 , 33 claim 1 , 34 and 35.3. An isolated polynucleotide capable of down-regulating expression of the isolated polynucleotide of in a host cell.4. A nucleic acid construct comprising the isolated polynucleotide of claim 1 , and a cis acting regulatory element for directing transcription of said nucleic acid sequence in a host cell.5. An isolated polynucleotide primer pair capable of specifically amplifying the isolated polynucleotide of .6. A plant cell exogenously expressing the isolated polynucleotide of .7. An isolated polypeptide comprising an amino acid sequence having at least 60% identity to a polypeptide selected from the group consisting of SEQ ID NO:2 claim 1 , 4 claim 1 , 5 claim 1 , 6 claim 1 , 8 claim 1 , 9 10 claim 1 , 28 and 29 claim 1 , wherein said polypeptide is capable of modulating acidity of a plant.8. A plant cell exogenously expressing the polypeptide of .9. A plant comprising the nucleic acid construct of .10. A food or feed comprising the plant of or a part thereof.11. A method of generating a transgenic plant claim 1 , comprising expressing within the plant the isolated polynucleotide of claim 1 , thereby generating the transgenic plant.12. A method of regulating ...

METHODS OF PRODUCING MOGROSIDES AND COMPOSITIONS COMPRISING SAME AND USES THEREOF

Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol. 1. A method of synthesizing a mogrol or mogrol precursor product from a mogrol precursor substrate , the method comprising contacting at least one mogrol precursor substrate with a mogroside pathway enzyme , wherein:(a) when said mogrol precursor product comprises diepoxy squalene and said mogrol precursor substrate comprises squalene or oxidosqualene, said mogroside pathway enzyme comprises a squalene epoxidase polypeptide at least 94% identical to SEQ ID NO: 14 or 89% identical to SEQ ID NO: 16, wherein said polypeptide catalyzes diepoxysqualene synthesis from squalene or oxidosqualene, thereby producing diepoxy squalene,(b) when said mogrol precursor product comprises 3 hydroxy, 24-25 epoxy cucurbitadienol and said mogrol precursor substrate comprises diepoxy squalene, said mogrol pathway enzyme comprises a cucurbitadienol synthetase polypeptide at least 60% homologous or ...

Isolated polynucleotides and methods and plants using same for regulating plant acidity

Provided are isolated polynucleotides comprising a nucleic acid sequence encoding a polypeptide having at least 60% identity to a polypeptide selected from the group consisting of SEQ ID NO:2, 4, 5, 6, 8, 9 10, 28 and 29, wherein the polypeptide modulates acidity of a plant. Also provided are nucleic acid constructs and plant cells comprising same and methods of using same for modulating acidity of a plant. Provided are isolated polynucleotides comprising a nucleic acid sequence encoding a polypeptide, wherein the polypeptide modulates acidity of a plant. Also provided are nucleic acid constructs and plant cells comprising same and methods of using same for modulating acidity of a plant.

Melon plants with enhanced fruit yields

Isolated polynucleotides and methods and plants using same for regulating plant acidity

Provided are isolated polynucleotides comprising a nucleic acid sequence encoding a polypeptide having at least 60% identity to a polypeptide selected from the group consisting of SEQ ID NO:2, 4, 5, 6, 8, 9 10, 28 and 29, wherein the polypeptide modulates acidity of a plant. Also provided are nucleic acid constructs and plant cells comprising same and methods of using same for modulating acidity of a plant. Provided are isolated polynucleotides comprising a nucleic acid sequence encoding a polypeptide, wherein the polypeptide modulates acidity of a plant. Also provided are nucleic acid constructs and plant cells comprising same and methods of using same for modulating acidity of a plant.

Methods of producing mogrosides and compositions comprising same and uses thereof

Melon plants with enhanced fruit yields

A Cucumis melo plant or a part thereof, the plant bearing more than 12 fruit, the fruit being seedless. Methods of generating same and breeding same are also disclosed.

Isolated polynucleotides and methods and plants that use them to regulate the acidity of plants

Una célula de planta o una planta que comprende una construcción de ácido nucleico que comprende una secuencia de ácido nucleico que codifica un polipéptido que tiene al menos un 80% de identidad con un polipéptido como se muestra en la SEQ ID NO:2 y un elemento regulador que actúa en cis para dirigir la transcripción de dicha secuencia de ácido nucleico en dicha planta, en donde dicho polipéptido modula la acidez de la planta y en donde la planta es de una familia de plantas seleccionada del grupo que consiste en Solanaceae, Cucurbitaceae, Rutaceae, Rosaceae, y Vitaceae. A plant cell or a plant comprising a nucleic acid construct comprising a nucleic acid sequence encoding a polypeptide that has at least 80% identity to a polypeptide as shown in SEQ ID NO: 2 and an element A cis-acting regulator to direct the transcription of said nucleic acid sequence in said plant, wherein said polypeptide modulates the acidity of the plant and wherein the plant is from a plant family selected from the group consisting of Solanaceae, Cucurbitaceae, Rutaceae, Rosaceae, and Vitaceae.

Melon plants with enhanced fruit yields

Melon plants with enhanced fruit yields.

Una planta de Cucumis melo o una parte de la misma, albergando la planta más de 12 frutos, siendo los frutos sin semillas. También se describen métodos de generación de la misma y de reproducción de la misma.

Methods of producing mogrosides and compositions comprising same and uses thereof

Methods of producing mogrosides and compositions comprising same and uses thereof

Melon plants comprising tetra-cis-lycopene

A Cucumis melo plant is disclosed, wherein a flesh of a fruit of the plant comprises tetra-cis-lycopene (pro-lycopene). Methods of generating same are also disclosed.

Cells comprising mogroside pathway enzymes and uses thereof

Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.

Isolated polynucleotides and methods and plants using same for regulating plant acidity

Provided are isolated polynucleotides comprising a nucleic acid sequence encoding a polypeptide, wherein the polypeptide modulates acidity of a plant. Also provided are nucleic acid constructs and plant cells comprising same and methods of using same for modulating acidity of a plant.