Cotton Minam dry seed sowing method

The invention relates to the crop cultivation field, in particular to a cotton Minam dry seed sowing method. The method comprises the following steps: 1) a cotton Minam area and a block are selected; 2) soil preparation ridging or flat land ditching are carried out; 3) dry seeds are sowed and blinding is carried out, etc. The ridging tectorial membrane drilling and dry seed sowing method is suitable for the Minam area which is insufficient in temperature-light resources, and the flat land ditching deficient entropy open field and dry seed sowing method is suitable for the Minam area which is sufficient in temperature-light resources. In rainy days, the dry seed sowing can alleviate the seed decay, the bud decay and the seedling death. In cotton field where soil is deficient, water conservancy conditions are deficient and the watering can not be carried out after the sowing in time, the dry seed sowing can avoid phenomenon that seeds loss viability caused by that intracellular water ofsoaked ...

Low position irrigation method for cotton planted in southern areas

The present invention relates to a low position irrigation method for cotton planted in southern areas, belonging to the field of crop cultivation. The low position irrigation method for cotton planted in southern areas comprises the following steps:1) paving a pipe network, all polyvinyl chloride (PVC) pipes are perpendicular to cotton rows, the PVC pipes are horizontally connected by tee joints, one end of each of the tee joints is connected with a hose, water spray holes are previously arranged on both the upper and the lower planes of the hose, primary switches are arranged at the water inlets of all rows of PVC pipes, secondary switches are arranged at the connecting part of all hoses with the PVC pipes, and the hoses are arranged between every two cotton ridges in parallel with the cotton rows; 2) irrigating, 5 to 7 secondary switches are first opened, and then, the primary switches are opened. Irrigation water and labours are reduced, and irrigation efficiency and quality are improved ...

Gene Gbvdr5 giving verticillium resistance in plants and use thereof

The invention relates to a gene Gbvdr5 giving verticillium resistance in plants and use thereof and belongs to the field of biological techniques. The gene Gbvdr5 is a surface receptor protein gene obtained from a variety H7124 of gossypium barbadense which is a verticillium-resistance material, wherein the nucleotide sequence of the gene Gbvdr5 is represented by SEQ ID No.1 and the amino acid sequence coded by the gene is represented by SEQ ID No.2. The gene has 12 leucine-rich repeat (LRR) conserved structure regions, and the N and C terminals of the gene both have a transmembrane structure region. The verticillium resistance in gene Gbvdr5 over-expression plants are much high, so the plants can delay incidence of a disease and the disease index of the plants is lowered considerably. After pathogenic bacteria are inoculated for 17, 20, 23 and 26 days, the verticillium resistance including resistance to defoliating type verticillium dahlia V991 and resistance to nondefoliating type verticillium ...

Light transceiving integrated device applied to fiber sensing

The embodiment of the invention provides a light transceiving integrated device applied to fiber sensing. The light transceiving integrated device comprises a light source, a photoelectric detector, a polarizing beam splitter, a half-wave plate, a Faraday rotator, a reflector and a lens, wherein the polarization state of emergent light emitted by the light source is regulated through the half-wave plate and the Faraday rotator so that the emergent light is changed between parallel transmission light and vertical reflected light; and the emergent light after the polarizing regulation enters tail fibers of polarization maintaining fibers through the polarizing beam splitter, the reflector and the lens, and the emergent light is received through the photoelectric detector and returned from an interferometer sensor. The structure and the light path process can realize integration and encapsulation of discrete components in a single module, reduce the fiber welding point number in a fiber gyro ...

Method for separating cotton chromosomes

The invention belongs to the technical field of molecular cytogenetics and particularly relates to a method for separating cotton chromosomes, which comprises the following steps: preparing material, preprocessing the material, performing primary hypotonic treatment, performing enzymolysis, performing secondary hypotonic treatment, drying and covering a plate, removing the cover plate, performing chromosome microisolation and collecting the chromosomes. In the method of the invention, the primary hypotonic treatment, emzymolysis, secondary hypotonic treatment and plate covering method are combined to prepare for making a chromosome membrane sheet; cell walls are completely removed; the chromosomes well disperse after being compressed slightly; and the shapes of the chromosomes are so clear as to facilitate the recognition of target chromosomes.

Cotton verticillium wilt resistant related gene GhVdr2 and application thereof

The invention relates to a cotton verticillium wilt resistant related gene GhVdr2 and application thereof, and belongs to the technical field of biology. The GhVdr2 gene is a surface receptor protein gene obtained from Gossypum hirsutum L. serving as a verticillium wilt resistant material, the gene contains 11 leucine-rich repeat (LRR) conserved domains, and a trans-membrane domain is at the end N and the end C respectively. Reverse transcription-polymerase chain reaction (RT-PCR) analysis discovers that the expression quantity of the GhVdr2 in roots is slightly higher than that in other organs; after deciduous and non-deciduous strong pathogenic strains V991 and Bp2 are treated for 4 days, the expression quantity of the GhVdr2 in roots, stems and leaves are increased; and the stress of metal ions, salts, drought and the like cannot increase the expression quantity of the GhVdr2. The verticillium wilt resistance of the GhVdr2 gene over-expression plant is obviously enhanced; after pathogenic ...

Cotton DNA fibre hybridization in situ technique

Promoter efficiently expressing in cotton root and application thereof

The invention relates to a plant gene promoter and an application thereof, belonging to the field of biotechnology. The invention obtains a promoter pgbv-5 of a Gossypium barbadense verticillium wilt resistance-related gene Ve-5. Place analysis shows that the promoter contains a disease resistance-related regulatory element, salicylic acid, auxin, gibberellic acid, ethylene, methyl jasmonate and other hormone-induced elements. Meanwhile, the promoter sequence also contains a number of light regulatory elements. The promoter allows efficient expression of a target gene at root, especially, theexpression level at root cap, growing point and partial elongation region is the highest. The expression level is very low or is nearly zero in leaves, flowers and legume tissues. After Arabidopsis thaliana plant is treated by salicylic acid, gibberellin or indoleacetic acid, GUS (beta-glucuronidase) activity is remarkably increased, which indicates that the promoter is a root hormone-induced promoter ...

Preparation for cotton DNA fibre by cutting and drainage method

The present invention provides process of preparing DNA fiber. In the preparation process, DNA fiber is stretched in a drainage process to avoid the influence of surface tension of liquid and the DNA fiber accumulation and breaking caused by inhomogeneous stress of the glass slide, so as to obtain straight homogeneous DNA fiber.

Light transceiving integrated device applied to fiber sensing

The embodiment of the invention provides a light transceiving integrated device applied to fiber sensing. The light transceiving integrated device comprises a light source, a photoelectric detector, a polarizing beam splitter, a half-wave plate, a Faraday rotator, a reflector and a lens, wherein the polarization state of emergent light emitted by the light source is regulated through the half-wave plate and the Faraday rotator so that the emergent light is changed between parallel transmission light and vertical reflected light; and the emergent light after the polarizing regulation enters tail fibers of polarization maintaining fibers through the polarizing beam splitter, the reflector and the lens, and the emergent light is received through the photoelectric detector and returned from an interferometer sensor. The structure and the light path process can realize integration and encapsulation of discrete components in a single module, reduce the fiber welding point number in a fiber gyro ...

Method for separating cotton chromosomes

The invention belongs to the technical field of molecular cytogenetics and particularly relates to a method for separating cotton chromosomes, which comprises the following steps: preparing material, preprocessing the material, performing primary hypotonic treatment, performing enzymolysis, performing secondary hypotonic treatment, drying and covering a plate, removing the cover plate, performingchromosome microisolation and collecting the chromosomes. In the method of the invention, the primary hypotonic treatment, emzymolysis, secondary hypotonic treatment and plate covering method are combined to prepare for making a chromosome membrane sheet; cell walls are completely removed; the chromosomes well disperse after being compressed slightly; and the shapes of the chromosomes are so clear as to facilitate the recognition of target chromosomes.

Method for preparing transgenic cotton by non-embryo transgenic process

Method for open-air leafless cutting of Hainan cotton

The invention relates to the biology of crop development, in particular to a method for open-air leafless cutting of Hainan cotton. The method comprises the step of inserting cotton branches into a cutting garden in Hainan Island, wherein, the moisture-holding capacity of the cutting garden reaches over 70 percent. The invention provides technical support to save the cotton which can not normally blossom and bear fruits, substantially solves the problem of high efficient cutting of cotton, improves the cutting survival rate to above 86 percent, and simplifies the requirements for cutting facilities. Moreover, the invention has the advantages of low cost, extremely simple operation, and easy control, promotion and application.

STABILIZED PLATFORM SYSTEM

A stabilized platform system includes a platform, a chassis subject to external vibration, first and second servomechanisms fixed to the chassis, first support having an end rotatably coupled to the first servomechanism and an other end rotatably coupled to the platform, a second support having an end rotatably coupled to the second servomechanism and an other end rotatably coupled to the platform, a third support having an end fixed relative to one of the chassis and an other end rotatably coupled to the platform, one or more sensors fixed to the platform, and a controller coupled to the one or more sensors and the first and the second servomechanisms. The controller causes the servomechanisms to adjust the inclination angles of the platform to maintain a desired orientation of the platform.

Cotton DNA in situ hybridization technique

The invention provides a method of hybridization in situ (Fiber-FISH) of cotton DNA fiber fluorescence. The invention adopts jackknife method to prepare karyon and drainage to pull the DNA fiber and then achieves hybridization in situ of DNA fiber fluorescence by using genome and 45S rDNA as the probes. During extracting the karyon by jackknife method, the phenols combined matter such as PVP40 isadded into the extracting solution and the hurtful medicine such as mercaptoethanol is avoided. The success rate reaches to a hundred percent. During pulling the DNA fiber by drainage, the influence of surface tension is avoided and the accumulation and fracture of the DNA fiber can be avoid, which are caused by uneven load when pushing the glass slide, therefore the prepared DNA fiber is lank, even and integrated. The genome and 45S rDNA label the probes by indentation translation method or random priming to achieve hybridization in situ of DNA fiber fluorescence and the legible and bright signal ...

A molecule marker of dominant non-gland genes of cotton

High-frequency induction and genetic transformation method for pear hairy roots of Agrobacterium rhizogene-mediated balsam

The invention relates to a high-frequency induction and genetic transformation method for pear hairy roots of Agrobacterium rhizogene-mediated balsam, comprising the following steps: selecting R1000 agrobacterium rhizogene strains to infect the stems or scutellum of seedlings in test tubes with the seedling age of 10-20 days, co-culturing in an MS culture medium containing IBA5.0 for 1-2 days to obtain co-cultured explants, and performing bacteria-free culture on the explants on a 1/2MS0 solid medium containing cephalosporin with the concentration of 500mg L<-1> for 8-10 days to obtain the balsam pear hairy roots. The balsam pear hairy roots acquired by the invention has the rooting frequency and rooting density, which respectively reaches 84.2% and 12.3%, and the total content of balsam pear protodioscin in the balsam is 33.43mg/g, which is respectively 69.1%, 48.6% and 49.8% higher than those in the root, stem and leaf of the seedling in a test tube. The method of the invention lays a ...

Cotton Minam dry seed sowing method

The invention relates to the crop cultivation field, in particular to a cotton Minam dry seed sowing method. The method comprises the following steps: 1) a cotton Minam area and a block are selected; 2) soil preparation ridging or flat land ditching are carried out; 3) dry seeds are sowed and blinding is carried out, etc. The ridging tectorial membrane drilling and dry seed sowing method is suitable for the Minam area which is insufficient in temperature-light resources, and the flat land ditching deficient entropy open field and dry seed sowing method is suitable for the Minam area which is sufficient in temperature-light resources. In rainy days, the dry seed sowing can alleviate the seed decay, the bud decay and the seedling death. In cotton field where soil is deficient, water conservancy conditions are deficient and the watering can not be carried out after the sowing in time, the dry seed sowing can avoid phenomenon that seeds loss viability caused by that intracellular water of soaked ...

Low position irrigation method for cotton planted in southern areas

The present invention relates to a low position sprinkling irrigation method for cotton planted in southern areas, belonging to the field of crop cultivation. The low position sprinkling irrigation method for cotton planted in southern areas comprises the following steps:1) paving a pipe network, all polyvinyl chloride (PVC) pipes are perpendicular to cotton rows, the PVC pipes are horizontally connected by tee joints, one end of each of the tee joints is connected with a hose, water spray holes are previously arranged on both the upper and the lower planes of the hose, primary switches are arranged at the water inlets of all rows of PVC pipes, secondary switches are arranged at the connecting part of all hoses with the PVC pipes, and the hoses are arranged between every two cotton ridges in parallel with the cotton rows; 2) irrigating, 5 to 7 secondary switches are first opened, and then, the primary switches are opened. Irrigation water and labours are reduced, and irrigation efficiency ...

Promoter efficiently expressing in cotton root and application thereof

The invention relates to a plant gene promoter and an application thereof, belonging to the field of biotechnology. The invention obtains a promoter pgbv-5 of a Gossypium barbadense verticillium wilt resistance-related gene Ve-5. Place analysis shows that the promoter contains a disease resistance-related regulatory element, salicylic acid, auxin, gibberellic acid, ethylene, methyl jasmonate and other hormone-induced elements. Meanwhile, the promoter sequence also contains a number of light regulatory elements. The promoter allows efficient expression of a target gene at root, especially, the expression level at root cap, growing point and partial elongation region is the highest. The expression level is very low or is nearly zero in leaves, flowers and legume tissues. After Arabidopsis thaliana plant is treated by salicylic acid, gibberellin or indoleacetic acid, GUS (beta-glucuronidase) activity is remarkably increased, which indicates that the promoter is a root hormone-induced promoter ...

Perultimate chromomere marker of cotton chromosome fluorescent in situ hybridization and use thereof

The present invention provides a telomere marker suitable for cotton fluorescent in situ hybridization, which is a sequence marked by biotin shown in the SEQ ID No.1. The telomere marker is taken as probe and cotton root tip somatic cell metaphase chromosome to perform fluorescence in situ hybridization (FISH), and stronger hybridization signals occur at the end of the chromosome. Further experiments confirm that the in situ hybridization signal is located at the extreme end of chromosome, that is, the hybridization signal is the telomere signal. Therefore, the length of the cotton somatic cell metaphase chromosome can be estimated by the telomere marker located at the end of chromosome, so as to lay the foundation for further karyotype analysis.

FISH method of one piece and multiple target of cotton

The invention belongs to the field of cytogenetics, specifically relating to a FISH method of the one piece and multiple target of the cotton, which comprises the following steps of: 1) putting chromosome samples of different cotton types on one carry sheet glass to produce a piece in a mixing way; and 2) simultaneously performing fluorescence in situ hybridization with the same probe, selecting and photographing the division phases of the chromosomes of the different cotton types under the same visual field when acquiring a fluorescence in situ hybridization result image. The method guarantees the consistency of external environment (the hybridizing or eluting temperature, humidity and time, and the content of each ion in solution, etc.), a manual operation step and the like, in the hybridization step of the target chromosomes of the different cotton types, and avoids the experimental error caused by the manual operation or the external condition when respectively performing the fluorescence ...