05-03-2020 дата публикации
Номер: US20200069717A1
Автор:
Chen Xin,
Chien Minchen,
GUO CHENG,
Hsieh Min-Kang,
Jockusch Steffen,
Ju Jingyue,
Kalachikov Sergey,
Kumar Shiv,
LI Xiaoxu,
Li Zengmin,
Ren Jianyi,
Russo James J.,
Shi Shundi,
Tao Chuanjuan
Disclosed herein, inter alia, are compounds, compositions, and methods of thereof in the sequencing a nucleic acid. 1. A method of incorporating a nucleotide analogue into a nucleic acid sequence comprising combining a thermophilic nucleic acid polymerase , a primer hybridized to nucleic acid template , and a nucleotide analogue comprising a detectable label , within a reaction vessel and allowing said thermophilic nucleic acid polymerase to incorporate said nucleotide analogue into said primer thereby incorporating a nucleotide analogue into a nucleic acid sequence34-. (canceled)5. The method of claim 2 , wherein Lis a substituted or unsubstituted methylene claim 2 , wherein Lis substituted with a substituted or unsubstituted C-Calkylene claim 2 , substituted or unsubstituted 2 to 6 membered heteroalkylene claim 2 , substituted or unsubstituted C-Ccycloalkylene claim 2 , substituted or unsubstituted 3 to 6 membered heterocycloalkylene claim 2 , substituted or unsubstituted phenyl claim 2 , or substituted or unsubstituted 5 to 6 membered heteroarylene.612-. (canceled)13. The method of claim 2 , wherein Lis a cleavable linker comprising a dialkylketal linker claim 2 , an azo linker claim 2 , an allyl linker claim 2 , a cyanoethyl linker claim 2 , a 1-(4 claim 2 ,4-dimethyl-2 claim 2 ,6-dioxocyclohex-1-ylidene) ethyl linker claim 2 , or a nitrobenzyl linker.14. The method of claim 2 , wherein:{'sup': 2', '2A', '2B', '2C', '2D', '2E, 'Lis L-L-L-L-L; and'}{'sup': 2A', '2B', '2C', '2D', '2E', '2A', '2B', '2C', '2D', '2E, 'L, L, L, L, and Lare independently a bond, —NN—, —NHC (O)—, —C(O) NH—, substituted or unsubstituted alkylene, substituted or unsubstituted heteroalkylene, substituted or unsubstituted cycloalkylene, substituted or unsubstituted heterocycloalkylene, substituted or unsubstituted arylene, or substituted or unsubstituted heteroarylene; wherein at least one of L, L, L, L, and Lis not a bond.'}1522-. (canceled)23. The method of claim 2 , wherein Lis a ...
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