01-02-2018 дата публикации
Номер: US20180028634A1
Принадлежит:
A method for treating a tumor includes administering (i) a vaccine that contains stage-specific embryonic antigen 4 conjugated to a carrier, (ii) an antibody that binds specifically to SSEA-4, and (iii) immune cells expressing a chimeric antigen receptor that specifically binds to SSEA-4. The tumor, which expresses SSEA-4, can be a breast, colon, gastrointestinal, kidney, lung, liver, ovarian, pancreatic, rectal, stomach, testicular, thymic, cervical, prostate, bladder, skin, nasopharyngeal, esophageal, oral, head and neck, bone, cartilage, muscle, lymph node, bone marrow, or brain tumor. 1. A method for treating a tumor , the method comprising administering to a subject having a tumor a vaccine that contains stage-specific embryonic antigen 4 (SSEA4) conjugated to a carrier , an antibody that binds specifically to SSEA-4 (anti-SSEA4 Ab) , and immune cells expressing a chimeric antigen receptor (CAR) that specifically binds to SSEA-4 , wherein the tumor expresses SSEA-4.2. The method of claim 1 , wherein the vaccine further contains α-galactosylceramide C34 or α-glucosylceramide C34 as an adjuvant.3. The method of claim 2 , wherein the immune cells are T cells claim 2 , NK cells claim 2 , NKT cells claim 2 , or a mixture thereof and the CAR contains (i) a scFv that binds specifically to SSEA4 and (ii) a CD3ζ endodomain or an FcεRIγ endodomain.4. The method of claim 3 , wherein the CAR further contains a second endodomain from CD28 claim 3 , CD137 claim 3 , CD4 claim 3 , OX40 claim 3 , ICOS claim 3 , Ly49D claim 3 , Ly49H claim 3 , KIR2DL4 claim 3 , KIR2DS1 claim 3 , KIR2DS2 claim 3 , KIR2DS3 claim 3 , KIR2DS4 claim 3 , KIR2DS5 claim 3 , KIR3DS1 claim 3 , NKG2C claim 3 , NKG2E claim 3 , NKG2D claim 3 , NKp30 claim 3 , NKp44 claim 3 , NKp46 claim 3 , NKp80 claim 3 , DNAM-1 claim 3 , or PILR.5. The method of claim 4 , wherein the vaccine claim 4 , the anti-SSEA4 Ab claim 4 , and the immune cells are administered sequentially.6. The method of claim 5 , wherein the tumor ...
Подробнее