18-02-2015 дата публикации
Номер: CN104359883A
Принадлежит:
The invention discloses an active fluorescence detection method of an anthropogenic silent information regulatory factor 6 (Sirtuin6). A polypeptide containing fluorophore and quenching group (FRET (fluorescence resonance energy transfer) effect) and long-chain fatty acylation lysine residue is used as a substrate, the fluorescence intensity is associated with the enzymatic activity of Sirtuin6, and the Sirtuin6 recognition site (long-chain fatty acylation lysine) of the substrate is located in the middle of the substrate by the FRET effect, thus, the substrate (polypeptide) is close to a natural substrate of Sirtuin6, and the combination is close; more fluorophores can be selected, and reliable screening result can be obtained. The method can be applied to screening of the Sirtuin6 regulator or is used for active detection of Sirtuin6 in a biological sample, has the characteristics of miniaturization, automation, reliability, rapidness and the like, is suitable for high-reflux application ...
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