19-07-2018 дата публикации
Номер: US20180201968A1
Принадлежит:
The invention relates to a method of converting an azidomethyl ether substituent to a free hydroxyl group. The invention also relates to methods of nucleic acid synthesis and sequencing comprising the use of nucleotide triphosphates having a 3′-O-azidomethyl substituent, to kits comprising nucleotide triphosphates having a 3′-O-azidomethyl substituent and photoactivatable transition metal complex and to the use of said kits in methods of nucleic acid synthesis and sequencing. 1. A method of converting an azidomethyl ether substituent to a free hydroxyl group wherein said method comprises the step of exposing a compound having said azidomethyl ether substituent to a photoactivated transition metal complex.2. The method as defined in claim 1 , wherein the photoactivated transition metal complex comprises a transition metal selected from ruthenium claim 1 , platinum claim 1 , palladium claim 1 , rhodium and osmium.3. The method as defined in claim 2 , wherein the transition metal is ruthenium.4. The method as defined in any one of to claim 2 , wherein the photoactivated transition metal complex comprises a ligand which is a mono-dentate or bidentate ligand selected from phosphine claim 2 , thiocynate claim 2 , nitrogen claim 2 , pyridine claim 2 , phenanthroline claim 2 , cyclopentadienyl and N-heterocyclic carbine based ligands.5. The method as defined in claim 4 , wherein the photoactivated transition metal complex comprises a pyridine ligand claim 4 , such as a bipyridine ligand.6. The method as defined in claim 5 , wherein the photoactivated transition metal complex is tris(2 claim 5 ,2′-bipyridyl)ruthenium(II)).7. The method as defined in any one of to claim 5 , wherein the azidomethyl ether is present on a ribose or deoxyribose sugar moiety.8. The method as defined in claim 7 , wherein the azidomethyl ether is a 2′ or 3′-O-azidomethyl.9. The method as defined in any one of to claim 7 , which comprises the step of exposing a compound having said azidomethyl ether ...
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