VERFAHREN ZUR FAERBUNG VON NICHTRADIOAKTIVEN MARKIERTEN NUKLEINSAEUREN UND ZUSAMMENSETZUNG ZUM GEBRAUCH IN DIESEM VERFAHREN.

Vorrichtung und Verfahren zum verschlüsselten Sicherheitsmarkierung zum Schutz von Wertgegenständen.

Vorrichtung und Verfahren zum verschlüsselten Sicherheitsmarkierung zum Schutz von Wertgegenständen.

Process for staining nucleic acids detected by non-radioactive labelling and set of reagents for implementing this process

Procédé de coloration d'une séquence d'acide nucléique fixée sur un support solide, dans lequel on utilise un système enzymatique propre à se fixer sur cette séquence pour la détecter par un marquage de type non radioactif et on fait réagir ce système enzymatique sur un substrat chromogénique pour former un produit coloré. On utilise une solution de révélation comprenant un composé organique soufré formant substrat, propre à être hydrolysé par l'enzyme pour former un composé organique à fonction thiol, ainsi qu'un composé d'un métal, autre que l'or, soluble et stable en solution aqueuse, propre à réagir avec le composé organique à fonction thiol pour former un complexe métal-composé organique de coloration spécifique du complexe, qui précipite in situ.

PROCEDURE FOR THE MARKING OF A NUCLEINSÄURE PROBE AND A TEST KIT FOR THE EXECUTION OF THIS PROCEDURE

Process of nonradioactive sequencing of DNA

A sequencing method for a DNA fragment is characterized in that: a) at least one DNA fragment containing the zone to be sequenced is isolated; b) direct sequencing of the said zone is carried out without using a radioactive compound; c) the fragments obtained by sequencing are migrated on a support; d) the DNA fragments are revealed after migration on the support or on a transfer support by placing them in the presence of a component having DNA affinity and comprising a detectable marker element; e) the detected DNA are read to determine the sequence of the DNA fragment.

Elektrophoresegerät

DEVICE AND PROCEDURE FOR CODED SAFETY MARKING TO THE PROTECTION OF OBJECTS OF VALUE.

PROCEDURES FOR FAERBUNG OF NICHTRADIOAKTIVEN MARKED NUCLEIC ACIDS AND COMPOSITION TO THE USE IN THIS PROCEDURE.

Device of electrophoresis

An electrophoresis device having a single-piece cartridge (10) filled with a suitable and optionally dyed gel (26) and made from a section of a hollow shape having two opposite open sides (20,22), one of which is sealed by a removable comb element (28) for forming imprints in the gel (26), while the other is sealed by a removable cover (36).

VERFAHREN ZUR FAERBUNG VON NICHTRADIOAKTIVEN MARKIERTEN NUKLEINSAEUREN UND ZUSAMMENSETZUNG ZUM GEBRAUCH IN DIESEM VERFAHREN.

Verfahren zur Markierung einer Nucleinsäure-Sonde und Reagenzienkit zur Ausführung dieses Verfahrens

Elektrophoresegerät

STAINING OF NUCLEIC ACID DETECTED WITH NONRADIOACTIVE LABEL AND REACTANT FOR CARRYING OUT THE SAME METHOD

PURPOSE: To enable the easy diagnosis of fetal genetic disease, a cancerous cell, etc., by staining a nucleic acid sequence immobilized on a fixed solid support according to a non-radioactive labeling method using an enzymatic system. CONSTITUTION: A desired nucleic acid is treated with a restriction enzyme, immobilized on a solid support such as a nitrocellulose membrane and labeled to provide (A) an immobilized nucleic acid. An enzyme having a probe complementary to a target nucleic acid sequence of the component A is selected to afford (B) an enzymatic system such as an alkaline phosphatase. A Tris-acetate, (pH 9.5) in an amount of 50 mM is formulated with 10 mM magnesium acetate, 5 mM cystamine-S-phosphate and 3 mM aurothioglucose containing an organometallic compound (e.g. a gold compound) to provide (C) a developer solution. The components A, B and C are then reacted in an aqueous solution to form (D) a precipitated stained substance (a metal-sulfur compound complex) on the solid ...

CRYPTO-MARKING OF VALUABLE OBJECT AND TOOL THEREFOR

PURPOSE: To carry out the crypto-marking of a valuable object useful for including a fluid target nucleic acid solution of a selected sequence capable of forming a marking pattern, as necessary, detecting the nucleic acid and protecting the valuables such as artistic works or documents from theft, resale or forgery. CONSTITUTION: When an article requiring a marking is coated with a target compound suitable for subsequent detection by an appropriate detection means to apply the crypto-marking for protecting the valuable object. A solution of a target nucleic acid, having a sequence selected so as to form a marking pattern and possessing the fluidity is prepared and a medium 10 composed of a membrane of nitrocellulose, etc., is then impregnated with an adequate amount of the resultant solution by using a coating means 11, etc. The medium 10 is then contained in each article 16 or a document 18 to be marked and the article is identified or authenticated by using a means for detecting the nucleic ...

PROCESS FOR LABELING A NUCLEIC-ACID SEQUENCE SONDE PROBE AND REAGENT KITS FOR CARRYING OUT THE PROCESS

PURPOSE: To achieve the labeling for the nucleic acid sequence sonde probe in no need of a radioactive substance and a DNA synthesizer by effecting the chemical crosslinking of the revelation enzyme on the modified nucleic-acid that is inhibited itself from hybridizing with target nucleic acid sequence and is bonded to the sonde nucleic acid sequence. CONSTITUTION: In the subject method, the labeling is accomplished by direct chemical crosslinking of the revelation system to the guanine or thymine group in a modified single-stranded nucleic acid which is itself incapable of hybridizing to the target sequence and which is linked to the probe sequence by ligation or covalent or hydrogen bonding. The direct chemical crosslinking of the revelation enzyme to the modified nucleic acid is preferably carried out by using a compound that can perfectly inhibit the formation of hydrogen bonds with a single stranded base chain in the double stranded nucleic acid. COPYRIGHT: (C)1990,JPO ...

METHOD AND DEVICE FOR THE DETECTION OF DNA AND RNA VIRAL GENOMES IN BIOLOGICAL MEDIA, IN PARTICULAR IN THE BLOOD SERUM

DISPOSITIF POUR LA DETECTION DE GENOMES VIRAUX A ADN ET ARN DANS UN ECHANTILLON DE MILIEU BIOLOGIQUE SUSCEPTIBLE D'ETRE CONTAMINE PAR DES PARTICULES VIRALES. ON TRAITE D'ABORD L'ECHANTILLON POUR DIGERER LES ACIDES NUCLEIQUES ADN ET ARN NON VIRAUX ET PRESERVER LES ACIDES NUCLEIQUES VIRAUX. ON PURIFIE ENSUITE, S'IL Y A LIEU, L'ECHANTILLON, PAR EXEMPLE EN LE FAISANT PASSER A TRAVERS UNE CARTOUCHE 32 CONTENANT UN GEL POUR RETENIR LES PROTEINES PRESENTES DANS L'ECHANTILLON. LES ACIDES NUCLEIQUES VIRAUX SONT ENSUITE EXTRAITS ET DENATURES EN PRESENCE D'UN MELANGE D'EXTRACTION DANS UN RESERVOIR 62. ILS SONT ENSUITE RECUEILLIS PUIS DETECTES PAR UN PROCEDE APPROPRIE, PAR EXEMPLE PAR UN PROCEDE COLORIMETRIQUE. L'INVENTION PERMET DE REALISER DE MANIERE PARTICULIEREMENT SIMPLE LA DETECTION POLYSPECIFIQUE DES GENOMES VIRAUX A ADN ET A ARN, NOTAMMENT DANS DES MILIEUX BIOLOGIQUES TELS QUE LE SERUM. DEVICE FOR THE DETECTION OF VIRAL DNA AND RNA GENOMES IN A SAMPLE OF BIOLOGICAL MEDIA LIKELY TO BE CONTAMINATED BY VIRAL PARTICLES. THE SAMPLE IS FIRST TREATED TO DIGERATE NUCLEIC ACIDS NON-VIRAL DNA AND RNA AND PRESERVE VIRAL NUCLEIC ACIDS. THE SAMPLE IS THEN PURIFIED, IF APPLICABLE, FOR EXAMPLE BY PASSING IT THROUGH A 32 CARTRIDGE CONTAINING A GEL TO RETAIN THE PROTEINS PRESENTED IN THE SAMPLE. THE VIRAL NUCLEIC ACIDS ARE THEN EXTRACTED AND DENATURE IN THE PRESENCE OF AN EXTRACTION MIXTURE IN A TANK 62. THEY ARE THEN COLLECTED THEN DETECTED BY A APPROPRIATE PROCESS, FOR EXAMPLE BY A COLORIMETRIC PROCESS. THE INVENTION ALLOWS IN A PARTICULARLY SIMPLE MANNER THE POLYSPECIFIC DETECTION OF VIRAL DNA AND RNA GENOMES, IN PARTICULAR IN BIOLOGICAL MEDIA SUCH AS SERUM.

Method and apparatus for high security crypto-marking for protecting valuable objects

A high security crypto-marking method for protecting valuable objects in which a target chemical compound suitable for subsequent detection by an appropriate detection means is applied to an object to be marked, wherein the method comprises the following operations: a) preparing a solution of a target nucleic acid having a sequence selected to form a marking pattern and possessing a selected degree of fluidity; b) incorporating a chosen quantity of the solution in each object of a class of objects to be marked; thereby making it possible, subsequently, and should the occasion arise, to perform the following operation: c) identifying or authenticating the object by using means for detecting the nucleic acid in order to reveal the marking. The invention can be used, where required, to provide irrefutable proof of ownership of an object in the event of theft, resale, or forgery.

NON-RADIOACTIVE DNA SEQUENCING PROCESS.

ELECTROPHORESIS DEVICE.

HIGH SECURITY ENCRYPTED MARKING METHOD AND DEVICE FOR THE PROTECTION OF VALUABLE OBJECTS

HIGH SECURITY CRYPT MARKING METHOD AND DEVICE FOR PROTECTING VALUE OBJECTS

L'invention concerne un procédé de marquage crypté de haute sécurité pour la protection d'objets de valeur, tels que des oeuvres d'art, des biens d'équipement, des documents officiels, etc., ce procédé consistant à : a) préparer une solution d'un acide nucléique-cible de séquence choisie, formant motif de marquage et possédant un degré de fluidité choisi, b) incorporer une quantité choisie de ladite solution à chaque objet d'une classe d'objets à marquer, ce qui permet de procéder ultérieurement, en cas de besoin, à une opération d'identification ou d'authentification de l'objet en utilisant un moyen de détection dudit acide nucléique pour révéler le marquage. L'invention permet, en cas de besoin, d'apporter la preuve irréfutable de la propriété d'un objet en cas de vol, revente ou contrefaçon. The invention relates to a high security encrypted marking method for the protection of valuables, such as works of art, capital goods, official documents, etc., the method comprising: a) preparing a solution of a target nucleic acid of chosen sequence, forming a marking motif and having a chosen degree of fluidity, b) incorporating a chosen quantity of said solution into each object of a class of objects to be marked, which allows to subsequently carry out, if necessary, an operation of identification or authentication of the object by using a means of detecting said nucleic acid to reveal the marking. The invention makes it possible, if necessary, to provide irrefutable proof of the ownership of an object in the event of theft, resale or counterfeiting.

Method and device for high-security encyphered marking to protect high-value objects

The invention relates to a method for high-security enciphered marking to protect high-value objects, such as works of art, equipment goods, official documents, etc., this method consisting in:   a) preparing a solution of a target nucleic acid of a chosen sequence, forming a marking pattern and possessing a chosen degree of fluidity,   b) incorporating a chosen quantity of the said solution into each object of a class of objects to be marked, which makes it possible to subsequently carry out, if needs be, an operation   c) of identification or authentication of the object by using a means of detection of the said nucleic acid in order to reveal the marking. <??>The invention makes it possible, if needs be, to produce irrefutable proof of the ownership of an object in the case of theft, resale or counterfeit.

METHOD FOR COLORING DETECTED NUCLEIC ACIDS BY NON-RADIOACTIVE LABELING AND REAGENT ASSEMBLY FOR CARRYING OUT SAID METHOD.

Method for staining nucleic acid detected by non-radioactive labelling and kit for use in this method

Method for staining a nucleic acid sequence bound to a solid support, in which an enzyme system capable of being bound to this sequence is used to detect it by a labelling of the non-radioactive type, and this enzyme system is reacted with a chromogenic substrate to form a coloured product. A visualising solution is used, comprising an organic sulphur compound forming a substrate capable of being hydrolysed by the enzyme to form an organic compound containing a thiol function, as well as a metal compound, for example a gold compound, which is soluble and stable in aqueous solution, capable of reacting with the organic compound containing a thiol function to form a metal-organic compound complex having a coloration specific to the complex, which precipitates in situ.

Electrophoresis device.

L'invention concerne un dispositif d'électrophorèse lequel comprenant une cassette monobloc (10) remplie d'un gel approprié (26), éventuellement coloré, et obtenue à partir d'un tronçon de profilé creux possédant deux faces ouvertes opposées (20, 22) dont l'une est obturée par un peigne amovible (28) propre à former des empreintes dans le gel (26) et l'autre par un opercule détachable (36). The invention relates to an electrophoresis device which comprises a one-piece cassette (10) filled with a suitable gel (26), optionally colored, and obtained from a section of hollow profile having two opposite open faces (20, 22 ) one of which is closed off by a removable comb (28) capable of forming imprints in the gel (26) and the other by a detachable cover (36).

Vorrichtung und verfahren zum verschluesselten sicherheitsmarkierung zum schutz von wertgegenstaenden.

Procede de coloration d'acides nucleiques detectes par marquage non radioactif et ensemble de reactifs pour la mise en óoeuvre de ce procede.

Procédé de coloration d'une séquence d'acide nucléique fixée sur un support solide, dans lequel on utilise un système enzymatique propre à se fixer sur cette séquence pour la détecter par un marquage de type non radioactif et on fait réagir ce système enzymatique sur un substrat chromogénique pour former un produit coloré. On utilise une solution de révélation comprenant un composé organique soufré formant substrat, propre à être hydrolysé par l'enzyme pour former un composé organique à fonction thiol, ainsi qu'un composé d'un métal, autre que l'or, soluble et stable en solution aqueuse, propre à réagir avec le composé organique à fonction thiol pour former un complexe métal-composé organique de coloration spécifique du complexe, qui précipite in situ.

Verfahren zur markierung einer nucleinsäure-sonde und reagenzienkit zur ausführung dieses verfahrens