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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 12. Отображено 12.
18-05-2011 дата публикации

Process for producing petroleum drilling machine brake block

Номер: CN0102061002A
Автор: ZHIMING LI, JIANYONG LEI, LI ZHIMING, LEI JIANYONG
Принадлежит:

The invention discloses a process for producing a petroleum drilling machine brake block. The brake block is manufactured through the steps of: threading for weaving; slicing; drying; dipping gum; shaping; pressing; carrying out heat treatment; grinding, and the like. The process effectively improves the wear resistance of the brake block and the stability of a brake process, reduces the decomposition of organic matters due to friction heat, has stable normal temperature and varietal temperature friction coefficient in the using process, reduces the abrasion and greatly prolongs the service life of the brake block.

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Номер записи: 1
13-07-2011 дата публикации

Fusion protein of human vascular endothelial growth factor 165b and human serum albumin, and preparation thereof

Номер: CN0102121024A
Автор: ZHU RUIYU, JIN JIAN, XIN XIN, ZANG XIAN, DAI HUIYUN, ZHANG DACHENG, LEI JIANYONG, CHEN YUN, RUIYU ZHU, JIAN JIN, XIAN ZANG, HUIYUN DAI, DACHENG ZHANG, JIANYONG LEI, YUN CHEN
Принадлежит:

The invention discloses a fusion protein of a human vascular endothelial growth factor 165b (hVEGF 165b) and human serum albumin (HSA) and preparation thereof, belonging to the technical field of long-acting recombinant fusion proteins. The fusion protein comprises a first region homologous with at least 85 percent of the sequence of the hVEGF 165b and a second region homologous with at least of 85 percent of the sequence of the HSA. The hVEGF 165b is spliced with the HAS by using a restriction enzyme digestion connection method without adding any connecting peptide between the hVEGF 165b and the HAS. The fusion protein can be used for carrying out substitution, deletion or addition of individual amino acid residues on the premise of no change of the characteristics of the fusion protein, can be expected to prolong the half-life period in vivo on the basis of maintaining the activity of the hVEGF 165b, and is a medical fusion protein which can replace the hVEGF 165b in the pharmacy field ...

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Номер записи: 2
23-02-2011 дата публикации

Method for preparing vasonatrin peptide (VNP) by genetic engineering recombination technology

Номер: CN0101979576A
Автор: JIAN JIN, YU JUN, PENG LI, CONG FANG, YUN CHEN, RUIYU ZHU, JIANYONG LEI, YUESHUI JIANG, MIAOZHANG ZHU, JIANKANG CHEN, JIN JIAN, LI PENG, FANG CONG, CHEN YUN, ZHU RUIYU, LEI JIANYONG, JIANG YUESHUI, ZHU MIAOZHANG, CHEN JIANKANG
Принадлежит:

The invention discloses a method for preparing vasonatrin peptide (VNP). In order to overcome the defects such high cost, low biological activity and the like in a conventional VNP chemical synthesis method, in the process of preparing the VNP by the method, a target gene is amplified by using polymerase chain reaction (PCR) technology; after performing enzyme cutting by taking pGEX-4T-1 as a vector plasmid, directionally inserting a VNP target gene to form a recombinant plasmid; Escherichia coli BL21 (DE3) serves as an expression strain; and a target protein is separated by using a GST affinity column. By using the method, a protein at a higher expression level can be obtained, and the protein with higher purity can be obtained by using affinity. The VNP obtained by utilizing the technology of the invention has higher biological activity and lower cost compared with the chemical synthesis method, which lays a foundation for further producing the VNP by a biological method.

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Номер записи: 3
13-07-2011 дата публикации

Preparation and application of OCH1 genetic flaw type P. pastoris X-33 bacterial strain

Номер: CN0102120967A
Автор: JIAN JIN, RUIYU ZHU, DACHENG ZHANG, YONGLI XU, JIANYONG LEI, YUN CHEN, JIN JIAN, ZHU RUIYU, ZHANG DACHENG, XU YONGLI, LEI JIANYONG, CHEN YUN
Принадлежит:

The invention discloses preparation and application of an alpha-1,6 mannose transferase gene (och1) flaw type P. pastoris X-33 bacterial strain, belonging to the technical field of biological engineering. The engineering bacterial strain P. pastoris X-33 (delta och1) is characterized in that a target gene knockout method by double-exchange homologous recombination is adopted, and a URA3 gene serves as a selective marker to knock out the alpha-1,6 mannose transferase gene (och1) of the P. pastoris X-33 (delta och1) to obtain the P. pastoris X-33 (delta och1) bacterial strain with OCH1 gene knockout. The bacterial strain provides a P. pastoris expression system which modifies protein by virtue of low N-glycosylation modification and provides a favourable basis for further glycosylation modification. The bacterial strain has the advantage of great potential application prospect.

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Номер записи: 4
13-07-2011 дата публикации

Method for improving expression level of Pichia pastoris recombinant protein

Номер: CN0102120979A
Автор: ZHU RUIYU, JIN JIAN, XIN XIN, ZHANG DACHENG, LEI JIANYONG, CHEN YUN, RUIYU ZHU, JIAN JIN, DACHENG ZHANG, JIANYONG LEI, YUN CHEN
Принадлежит:

The invention relates to a method for improving the expression level of Pichia pastoris recombinant protein. On the basis of the normal process flow of recombinant Pichia pastoris, trace melatonin is added to greatly improve the expression of the recombinant protein. By utilizing the method, radical accumulation in yeast brought by intimidation, such as unbalanced oxygen supply, during the high-density fermentation of the recombinant saccharomycete is lowered, and the damage on the yeast brought by the radical in the high-density fermentation process is eliminated so as to improve the physiological condition of the yeast. After the melatonin is used, the expression level of the recombinant protein is improved to a large extent.

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Номер записи: 5
07-04-2010 дата публикации

Fermentation method for recombinant Pichia pastoris

Номер: CN0101691595A
Автор: Zhu Ruiyu, Jin Jian, Lei Jianyong, Chen Yun, Li Ying, Chu Min
Принадлежит:

The invention relates to a novel fermentation method for recombinant Pichia pastoris. On the basis of growing the recombinant Pichia pastoris with glycerin, pulse methanol feeding is adopted to induce the expression of recombinant proteins. In the methanol feeding control, a methanol sensor is not required to be used; and simultaneously, defects of too low concentration of the methanol in the DO stat feeding inducing process, and methanol accumulation due to too high feeding speed and too low concentration of the methanol caused by too low feeding speed in the constant-speed intermittent constant-speed feeding inducing process are overcome. After the pulse methanol feeding is adopted, expression of recombinant fusion long-acting interferon is greatly improved.

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Номер записи: 6
13-07-2011 дата публикации

Construction and application of URA3 defective P. pastoris X-33 strain

Номер: CN0102120966A
Автор: RUIYU ZHU, JIAN JIN, DACHENG ZHANG, YONGLI XU, JIANYONG LEI, YUN CHEN, ZHU RUIYU, JIN JIAN, ZHANG DACHENG, XU YONGLI, LEI JIANYONG, CHEN YUN
Принадлежит:

The invention discloses construction and application of an orotidine-5'- phosphate decarboxylase (ura3) defective P. pastoris X-33 strain, belonging to the technical field of biologic engineering. The engineering strain P. pastoris X-33 (delta ura3) is obtained by a homologous recombination target gene knockout method. The strain can use the ura3 gene as a selected marker for constructing different kinds of engineering strains, and when the engineering strains are constructed, the carried ura3 genes are not required to be from the same microbial population. Simultaneously, the invention provides a better platform strain for performing subsequent engineering reconstruction such as glycosylation reconstruction on the X33, thus having great potential application prospect.

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Номер записи: 7
15-06-2011 дата публикации

Preparation method for fused protein of human C-type diuresis promotion sodium peptide and human serum albumins and its product

Номер: CN0101063123B
Автор: LI JIE, LEI JIANYONG, ZHANG LIANFEN, XU ZHENGHONG, DOU WENFANG, SHI ZHONGPING, JIN JIAN, CHEN WEI, JIE LI, JIANYONG LEI, LIANFEN ZHANG, ZHENGHONG XU, WENFANG DOU, ZHONGPING SHI, JIAN JIN, WEI CHEN
Принадлежит:

The invention discloses a preparing method of fusion protein of human C-type natriuretic peptide (CNP) and human serum albumin (HSA) and product, belonging to the technical field of long-acting recombinant fusion protein drug, which comprises the following steps: adopting overlapping PCR technique; splitting HSA cDNA and CNP cDNA; integrating HSA-CNP cDNA fusion gene obtained without any connecting peptide being added into chromosome of a host; and expressing in the host system. The fusion protein of the invention includes a first area with at least 85% sequence homologous with human serum albumin and the second area with at least 85% sequence homologous with human C-type natriuretic peptide, individual amino acid residue substitution, deletion and addition can be carried out with the characteristics of the fusion protein being not changed.The fusion protein of the invention has extended half-life in the human body, with the physiological property of the human C-type natriuretic peptide ...

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Номер записи: 8
28-07-2023 дата публикации

Community passing epidemic prevention monitoring joint defense system and method based on artificial intelligence

Номер: CN116503990A
Автор: LIAO YUTIAN, HE GUOTIAN, LI BENWANG, LEI JIANYONG, XIONG MIN, WANG SURAN
Принадлежит:

The invention discloses a community passing epidemic prevention monitoring joint defense system based on artificial intelligence, and relates to the technical field of community security and protection. A data acquisition device; a data arrangement device; an alarm device; a user terminal; wherein the monitoring device, the data acquisition device, the data arrangement device, the cloud server and the alarm device are in communication connection, and the cloud server is in wireless communication connection with the user terminal. According to the community passing epidemic prevention monitoring joint defense system based on artificial intelligence, through the cooperation relationship among the modules, the division of safety levels is realized, home-entry personnel can be divided into strange personnel, familiar personnel and residents, and then an alarm signal is selectively sent out.

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Номер записи: 9
15-08-2023 дата публикации

Intelligent health community platform construction method based on government big data

Номер: CN116595081A
Автор: LIAO YUTIAN, HE GUOTIAN, LI BENWANG, LEI JIANYONG, XIONG MIN, WANG SURAN
Принадлежит:

The invention relates to the technical field of big data processing, and particularly discloses a method for constructing a smart health community platform based on government big data, which is used for solving the technical problems of time consumption and complexity caused by manual labor in big data platform construction in the prior art. According to the technical key points, the method comprises the following steps: acquiring basic information of a data source, and reading a corresponding data dictionary into a database according to the basic information of the data source; constructing a target library table structure according to setting of a data source and a rule of a target platform, and writing target data into the target library table structure; and configuring an ETL job according to the target data, and scheduling and monitoring the operation of the ETL job. According to the method, the data dictionary of the data source can be automatically read into the database, the target ...

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Номер записи: 10
09-07-2014 дата публикации

Kit and PCR amplification method for detection of one pathogenic mutation site of male infertility Dmc1 gene

Номер: CN103911424A
Автор: ZHU WEI, HE XIONGLEI, PAN WUGUANG, GUO LEI, LEI JIANYONG, PAN MINMING
Принадлежит:

The invention discloses a kit and a PCR amplification method for detection of one pathogenic mutation site of a male infertility Dmc1 gene. The method is characterized in that a modified mutation-detection primer resistant to 3'-5' exonuclease enzyme digestion is used in a PCR reaction in which a high-fidelity DNA polymerase participates, so that the specificity of PCR is improved; and the existing condition of a gel electrophoresis product band can be directly observed to judge the genotype of the Dmc1 gene corresponding mutation site. The detection kit has low requirements on instruments and equipment, is simple in operation, is economic, has no need of sequencing, and can be operated in common molecular biology laboratories.

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Номер записи: 11
09-04-2014 дата публикации

Coniothyrium minitans strain JN-CM for preventing and controlling stalk break

Номер: CN103710269A
Автор: ZHU RUIYU, LEI JIANYONG, JIN JIAN, XIA SENYU, CHEN YUN, SHEN ZHISONG, XU MENGLONG, LI HONG
Принадлежит:

The invention belongs to the technical field of biological pesticides, and in particular relates to a coniothyrium minitans strain JN-CM for preventing and controlling stalk break. The coniothyrium minitans strain JN-CM is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC NO.8325. The invention also discloses a biological characteristic and a culture method of the coniothyrium minitans strain JN-CM. The microbial strain has a good biological prevention and control effect on the stalk break.

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Номер записи: 12