27-08-2015 дата публикации
Номер: US20150240252A1
Принадлежит:
Methods for modulating plants using optimized ZmSAUR down-regulation constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency, increased ear tissue growth or kernel number. 1. (canceled)2. (canceled)3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. (canceled)8. A plant or plant cell comprising an expression cassette effective for reducing expression of at least one endogenous short lived auxin responsive production gene , wherein said expression cassette comprises a promoter that functions in plants operably linked to a nucleic acid configured for RNA silencing or interference , wherein said nucleic acid is selected from the group which comprises:a. the polynucleotides of SEQ ID NO: 33, 38 and 43;b. an expression cassette consisting essentially of nucleotide sequences SEQ ID NO: 31, 36 or 41 separated from SEQ ID NO: 32, 37 or 42, wherein the nucleotide sequences are separated by an intervening polynucleotide;c. the expression cassette comprising an intervening polynucleotide that is a ZmAdh1 intron 6; and{'claim-ref': {'@idref': 'CLM-00018', 'claim 18'}, 'd. The expression cassette of , wherein the ZmAdh1 intron 6 sequence is bases 557-1093 of SEQ ID NO: 44.'}9. The plant cell of claim 8 , wherein the plant cell is from a dicot or monocot.10Brassica. The plant cell of claim 9 , wherein the dicot or monocot is maize claim 9 , wheat claim 9 , rice claim 9 , sorghum claim 9 , barley claim 9 , oat claim 9 , lawn grass claim 9 , rye claim 9 , soybean claim 9 , or sunflower.11. (canceled)12. The plant regenerated from the plant cell of claim 10 , wherein the plant exhibits one or more of the following: increased drought tolerance claim 10 , increased nitrogen utilization efficiency claim 10 , increased seed yield claim ...
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