23-06-2023 дата публикации
Номер: CN116286993A
Принадлежит:
The invention relates to the technical field of biological medicine, in particular to an establishment method and application of an HIV-tat nerve injury model. According to the specific technical scheme, the method for establishing the HIV-tat-induced nerve injury model comprises the steps that a tat gene sequence of HIV-1 is inserted into the upstream of an MLV envelope gene, a P2A sequence is introduced behind tat, tat and ENV are transcribed and expressed at the same time, after translation, P2A spontaneous cutting is conducted to form free tat protein and ENV protein, the established virus MLV-Tat can normally infect a mouse and express the Tat protein, and therefore the mouse can be normally infected with the Tat protein. The nerve injury is induced by the Tat protein, so that the nerve injury model is successfully constructed. Model construction only needs intraperitoneal injection of the MLV-tat virus, the damage to animals is small, and the operation is convenient.
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