01-12-2017 дата публикации
Номер: CN0107422112A
Автор:
LI ZHAOZHOU,
CHEN XIUJIN,
WANG YAO,
FAN ZHENJIANG,
GAO HONGLI,
LI DAOMIN,
LI SONGBIAO,
CAO LI,
LYU PU,
JIN DONGLIANG,
ZHANG WEIMIN
Принадлежит:
The invention relates to an immunoassay kit for detecting ethopabate, and a preparation method and application thereof, belonging to the field of immunological detection. The kit comprises an ELISA plate coated with an ethopabate antigen, an ethopabate monoclonal antibody solution, an enzyme-labeled or fluorescently-labeled goat anti-mouse secondary antibody, a standard ethopabate solution, a washing solution and a diluent, wherein the ethopabate antigen is a conjugate of ethopabate semiantigen and a vector, and the vector is one or two selected from a group consisting of ovalbumin, bovine serum albumin, thyroid protein, hemocyanin, human serum albumin and polylysine. When applied to detection of ethopabate residues in animal food, the kit has the advantages of high sensitivity, precision and accuracy, good specificity, low cross-reaction rate, good stability, long storage time, easy operation, quickness and suitability for on-site primary screening of large quantities of samples, and is ...
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