DEVICE FOR COLLECTING ORAL FLUID SAMPLES AND THE LIKE

Devices for collection and elution of oral fluid samples and the like enable collection and elution of samples by using a handle in the form of a rod having a collection pad thereon which is inserted into a collection tube. The collection tube has an adaptor with a narrowed portion associated therewith for squeezing the pad to wring a sample therefrom. In an alternative embodiment, the collection tube has a narrowed portion that squeezes the pad as the pad is inserted there though. Caps are provided to 1) support the rod in an assembly with the adaptor and collection tube to form an assembled sample collection device and 2) to close the collection tube after the handle and pad are removed from the collection tube subsequent to depositing the sample elution solution therein. The eluted sample can be used for detection of an analyte. 1. A sample collection device comprising:a cap with screw threads;a handle;a collection pad; an adaptor with a narrow passage; anda sample collection tube for mounting the adaptor and for receiving the handle with the collection pad mounted on the handle wherein the narrow passage of the adaptor squeezes the collection pad to remove material of the sample therefrom.2. The device of claim 1 , wherein one end of said handle is mounted to the cap.3. The device of claim 1 , wherein said handle is a rounded rod.4. The device of claim 1 , wherein said collection pad is wrapped around the handle at an end remote from the cap.5. The device of claim 1 , wherein the adaptor defines a narrow inner passage.6. The device of claim 1 , wherein the outer diameter of the handle portion wrapped with the collection pad is slightly smaller than the inner diameter of the narrow passage of said adaptor claim 1 , thereby allowing the collection pad to pass through the narrow passage to wring out the sample from the pad.7. The device of claim 1 , wherein said adaptor has male screw threads on one end and female screw threads on the other end.8. The device of ...

Luminescence methods and reagents for analyte detection

The present invention relates to chemiluminescent method and regent to detect analyte. One aspect of the current invention relates to using first analyte binding molecule labeled solid phase support encapsulated with chemiluminescent dye and chemiluminescent reaction generating molecule or enzyme coupled with second analyte binding molecules such as antibody HRP conjugate to detect specific analyte molecules. Another aspect of the current invention is that the analyte binding molecule labeled solid phase support encapsulated with chemiluminescent dye is magnetic. 1a) contacting said sample with a first ligand of the analyte coupled with luminescence producing enzyme and a second ligand of the analyte coupled with solid phase support encapsulated with chemiluminescent dye, andb) detecting the light generated from said luminescence.. A method to detecting analyte in a sample, comprising: This application is a Continuation-In-Part application of U.S. application Ser. No. 14/803,600, filed on Jul. 20, 2015, which is a Continuation application of application Ser. No. 12/924,072. It also claims priority to U.S. Provisional Patent Application No. 62/032,558 filed on Aug. 2, 2014. The entire disclosure of the prior application is considered to be part of the disclosure of the instant application and is hereby incorporated by reference.The present invention relates to chemiluminescent methods and regents to detect analyte. One aspect of the current invention relates to using first analyte binding molecule labeled solid phase support (such as antibody coated micro particles) encapsulated with chemiluminescent dye and chemiluminescent reaction triggering/activating molecule/enzyme coupled with second analyte binding molecules such as antibody HRP conjugate to detect specific analyte molecules.Chemiluminescence or chemoluminescence (herein sometimes referred to as CL) is the emission of light (luminescence) with limited emission of heat as the result of a chemical reaction. ...

DEVICE FOR COLLECTING ORAL FLUID SAMPLES AND THE LIKE

Devices for collection and elution of oral fluid samples and the like enable collection and elution of samples by using a handle in the form of a rod having a collection pad thereon which is inserted into a collection tube. The collection tube has an adaptor with a narrowed portion associated therewith for squeezing the pad to wring a sample therefrom. In an alternative embodiment, the collection tube has a narrowed portion that squeezes the pad as the pad is inserted there though. Caps are provided to 1) support the rod in an assembly with the adaptor and collection tube to form an assembled sample collection device and 2) to close the collection tube after the handle and pad are removed from the collection tube subsequent to depositing the sample elution solution therein. The eluted sample can be used for detection of an analyte. 1. A sample collection device comprising:a cap with screw threads;a handle;a collection pad; an adaptor with a narrow passage; anda sample collection tube for mounting the adaptor and for receiving the handle with the collection pad mounted on the handle wherein the narrow passage of the adaptor squeezes the collection pad to remove material of the sample therefrom.2. The device of claim 1 , wherein one end of said handle is mounted to the cap.3. The device of claim 1 , wherein said handle is a rounded rod.4. The device of claim 1 , wherein said collection pad is wrapped around the handle at an end remote from the cap.5. The device of claim 1 , wherein the adaptor defines a narrow inner passage.6. The device of claim 1 , wherein the outer diameter of the handle portion wrapped with the collection pad is slightly smaller than the inner diameter of the narrow passage of said adaptor claim 1 , thereby allowing the collection pad to pass through the narrow passage to wring out the sample from the pad.7. The device of claim 1 , wherein said adaptor has male screw threads on one end and female screw threads on the other end.8. The device of ...

Methods and devices for detection of resistance to an enzyme inhibitor

Embodiments described herein relate to assay methods and kits for detecting resistance of any enzyme to its inhibitor due to functional alteration of the enzyme, comprising, conducting two or more reactions with two or more reagent mixes optionally containing substrates for the enzyme. The mixes are substantially similar, except that one contains no enzyme inhibitor whereas the others contain an enzyme inhibitor being tested for resistance. The ratio of the signal from the reaction with an inhibitor to that from a reaction without an inhibitor is used to indicate whether the enzyme is resistant to the enzyme inhibitor and also determine the susceptibility or resistance of the enzyme to various inhibitors and further identify enzyme variants. Embodiments further relate to assay methods comprising only two reactions—one conducted in a mix containing the inhibitor and the other without the inhibitor. Further included are devices for conducting such assays.

CHEMILUMINESCENT METHODS AND REAGENTS FOR ANALYTE DETECTION

The present invention relates to chemiluminescent method and regent to detect analyte. One aspect of the current invention relates to using enzyme substrate that can be cleaved by target enzyme to release chemiluminescent compound giving light signal for the detection of varieties of target enzymes. Another aspect of the current invention relates to use chemiluminescent enzyme coupled with analyte binding molecules to detect specific analyte molecules in a homogenous phase. 2. A method to detecting analyte in a sample , comprising:a) contacting said sample with a first ligand of the analyte coupled with ATP producing enzyme and a second ligand of the analyte coupled with luciferase, wherein said luciferase use ATP as substrate; andb) detecting the light generated from said luciferase.3. A kit for detecting analyte in a sample , comprising a first ligand of the analyte coupled with ATP producing enzyme and a second ligand of the analyte coupled with luciferase. This application is the divisional application of U.S. application Ser. No. 12/287,916 filed on Oct. 15, 2008, which claims priority to U.S. Provisional Application No. 60/999,166 filed on Oct. 16, 2007. The entire disclosure of the prior application is considered to be part of the disclosure of the instant application and is hereby incorporated by reference.The present invention relates to chemiluminescent method and regent to detect analyte. One aspect of the current invention relates to using enzyme substrate that can be cleaved by target enzyme to release chemiluminescent compound giving light signal for the detection of varieties of target enzymes. Another aspect of the current invention relates to using chemiluminescent enzyme coupled with analyte binding molecules to detect specific analyte molecules in a homogenous phase.Advances in the biological, biomedical and pharmaceutical sciences have accelerated the pace of research and diagnostics to a level unparalleled to the past. With sequences of whole ...

Methods for multiplexed analyte detection

The methods and compositions provided herein are based on use of reporter system to detect multiple analyte in a sample. The reporter system can be a signal amplification system that includes a carrier, typically a particle containing an analyte binding moiety, and multiple copies of a signaling moiety. Different reporter system can bind with different analyte. Different reporter system or their signaling moiety can be distinguished and detected. In various embodiments, the signaling moiety is physically released from its carrier after the carrier has been bound to the analyte and distinguished and detected after the release (Figure 5).

Methods and devices for detection of resistance to an enzyme inhibitor

Methods and devices for detection of resistance to an enzyme inhibitor

Embodiments described herein relate to assay methods and kits for detecting resistance of any enzyme to its inhibitor due to functional alteration of the enzyme, comprising, conducting two or more reactions with two or more reagent mixes optionally containing substrates for the enzyme. The mixes are substantially similar, except that one contains no enzyme inhibitor whereas the others contain an enzyme inhibitor being tested for resistance. The ratio of the signal from the reaction with an inhibitor to that from a reaction without an inhibitor is used to indicate whether the enzyme is resistant to the enzyme inhibitor and also determine the susceptibility or resistance of the enzyme to various inhibitors and further identify enzyme variants. Embodiments further relate to assay methods comprising only two reactions- one conducted in a mix containing the inhibitor and the other without the inhibitor. Further included are devices for conducting such assays.

Chemiluminescent methods and reagents for analyte detection

The present invention relates to chemiluminescent method and regent to detect analyte. One aspect of the current invention relates to using enzyme substrate that can be cleaved by target enzyme to release chemiluminescent compound giving light signal for the detection of varieties of target enzymes. Another aspect of the current invention relates to use chemiluminescent enzyme coupled with analyte binding molecules to detect specific analyte molecules in a homogenous phase.

一种易于滑行的光栅尺读数头结构

本实用新型公开了一种易于滑行的光栅尺读数头结构，包括滑行座、弹性组件和滑块，滑行座的底部固定有主板，滑行座的顶部延伸出与尺身底部密封条接触的滑行导板，滑行导板的顶部设置有安装平面，安装平面上连接有弹性组件的一端，弹性组件的另一端连接滑块，滑块上设置相互连接的发射板和接收板，接收板上电连接有接插件，还包括软排线，软排线的一端与接插件可拆卸连接，另一端与主板固定电连接，滑行导板与密封条的接触面上设置有若干凸起，通过软排线的一端与接插件可拆卸连接，另一端与主板固定电连接，若滑块上的芯片损坏时，只需将软排线拆卸更换即可，滑行导板与密封条的接触面上设置有若干凸起，减小了与密封条的摩擦力，使得滑行更顺畅。