15-10-2008 дата публикации
Номер: CN0101285803A
Автор:
YANG BINGHU,
ZHAO XIUMEI,
CHAO KETU,
LIU YAQIN,
HOU YANG,
YOU LIHUA,
YE MENG,
NIE RAN,
SUN JIANHUI,
MA JIAHUA,
BINGHU YANG,
XIUMEI ZHAO,
KETU CHAO,
YAQIN LIU,
YANG HOU,
LIHUA YOU,
MENG YE,
RAN NIE,
JIANHUI SUN,
JIAHUA MA
Принадлежит:
The invention discloses a qualitative analysis detection method for illegally mixed high-polar chemical anti-diabetic components in anti-diabetic traditional Chinese medicine products. The method comprises the following steps that: 1) high efficient liquid phase chromatography conditions: an ammonium acetate-triethylamine-acetonitrile moving phase system and a C18 chromatographic column with certain specification are used, the wavelength is detected by ultraviolet, and the flow rate is 1.0ml/min; 2) analysis result: glibenclamide, glipizide, gliclazide, glimepiride, gliquidone, repaglinide, nateglinide, rosiglitazone and pioglitazone hydrochloride can realize the complete separation; 3) result judgment: when retention time of a chromatographic peak in an anti-diabetic traditional Chinese medicine product is consistent with that of anti-diabetic medicine in the step 2) and the apparent absorption is shown out, which indicate that the anti-diabetic medicine is contained in the sample to be ...
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