28-05-2014 дата публикации
Номер: CN103820513A
Принадлежит:
The invention discloses a method for synthesizing sialylated oligosaccharide and an analogue. The invention provides the method for synthesizing sialylated oligosaccharide and the analogue, the method comprises the followings steps: 1)uniformly mixing a donor compound, MgC12, pyruvate and an engineering bacteria E.coli delta nanTEK/pNA bacterium suspension, reacting, collecting a supernatant; 2)adding an acceptor compound, cytidine triphosphate, CMP-sialic acid synthetase and sialytransferase in the supernatant, uniformly mixing, reacting, and collecting the reaction product which is sialyloligosaccharide or its analogue. The experiment of the present invention proves that the method has the advantages that N-acetylneuraminic acid with low price is taken as a raw material, a method of whole-cell catalysis and a method of two-step enzymatic synthesis are combined, the sialyloligosaccharide and its analogue can be synthesized by an one-pot synthesis method, the synthesis cost of the compounds ...
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