19-08-2009 дата публикации
Номер: CN0101509038A
Принадлежит:
The invention discloses a breeding method of rice, the root of which becomes to be red when being processed by photoinduction. The method comprises special PCR primer design, extraction of DNA, PCR, endonuclease reaction and testing process; wherein, the nucleotide sequence from upstream end 5'-end 3' of the special PCR primer is CGTCCATTCACAGGGTA, and the nucleotide sequence from downstream end 5'-end 3' is CAGCAGATGAGGCAAACT. The special PCR primer designed by the invention can be used for amplifying DNA extracted in rice seedling stage, and amplified production can be identified by CAPS1 molecular marker after zymolysis by BamH I. Therefore, the seed selection method can rapidly and accurately select the novel rice having the DNA leading the root to become to be red when being processed by photoinduction, thus overcoming the defect that the existing breeding method has blindness and low efficiency, and having the advantages of high breading efficiency, strong direction and convenient ...
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