28-02-2019 дата публикации
Номер: US20190062412A1
Автор:
Chunlong LIU,
Jie PENG,
Zhou ZHANG,
Shuanzhu ZHAI,
Ning LI,
Yan SU,
Zeqi ZHOU,
LIU CHUNLONG,
PENG JIE,
ZHANG ZHOU,
ZHAI SHUANZHU,
LI NING,
SU YAN,
ZHOU ZEQI,
LIU, Chunlong,
PENG, Jie,
ZHANG, Zhou,
ZHAI, Shuanzhu,
LI, Ning,
SU, Yan,
ZHOU, Zeqi
Принадлежит:
The present invention provides a hybridoma cell under the accession number CGMCC No. 13827. The hybridoma cell is capable of producing a monoclonal antibody against galactomannan antigen and a kit is prepared using the same. The kit provided by the present invention can specifically bind to the GM antigen, has both sensitivity and specificity of more than 95%, a detection limit of 0.85 ng/mL compared to 1 ng/mL of the existing product, and high compliance rate between the detection result and the reference reagent, and can provide more accurate and reliable detection results, so that IA can be detected early in the course of the disease and the patients can receive treatment in timely and effective manner, thereby improving the survival rate of patients. Moreover, the kit has simple and convenient operation, rapid and sensitive detection, which provides an effective tool for the quantitative detection of GM antigen. 1. A hybridoma cell or a passage cell thereof having an accession number of CGMCC No. 13827.2Aspergillus. A monoclonal antibody or a specific antigen-binding fragment thereof claim 1 , wherein the monoclonal antibody is produced by the hybridoma cell of claim 1 , and the specific antigen-binding fragment is capable of specifically binding to galactomannan antigen.3. The monoclonal antibody or specific antigen-binding fragment thereof of claim 2 , wherein the specific antigen-binding fragment is selected from the group consisting of (Fab′)2 claim 2 , Fab claim 2 , Fv claim 2 , scFv claim 2 , diabody claim 2 , linear antibody or multispecific antibody.4. A detection reagent comprising the monoclonal antibody or specific antigen-binding fragment thereof of .5. The detection reagent of claim 4 , wherein the specific antigen-binding fragment is selected from the group consisting of (Fab′)2 claim 4 , Fab claim 4 , Fv claim 4 , scFv claim 4 , diabody claim 4 , linear antibody or multispecific antibody.6. A detection kit comprising the monoclonal antibody or ...
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