Настройки

Укажите год
-

Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

Подробнее
-

Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

Подробнее

Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Укажите год
Укажите год
Применить Всего найдено 2. Отображено 2.
12-11-2014 дата публикации

Seepage-proofing seawall

Номер: CN104141285A
Автор: ZENG ZHEN, YUAN WENXI, LUO FANGXIN, SHAO YANHUA, MAO DANHONG
Принадлежит:

Provided is a seepage-proofing seawall. The filling materials of the body of the seawall are mainly riprap, the middle portion of the body of the seawall is filled with filling sand, and concrete diaphragm walls are arranged in the filling sand. A filter bag contained rubble layer is arranged between the filling sand and the riprap, and a sand filled drainage layer is arranged on the bottom of the filling sand. The filling sand is filled through sand filling pipe bags. A double-row high pressure swing jet concrete diaphragm wall is adopted for each concrete diaphragm wall. Using of airtight earthwork is eliminated, the problem that airtight earthwork is difficult to fill is solved, construction duration can be shortened, project amount can be reduced, and therefore overall investment of a project can be saved; the section width of the body of the seawall is reduced, and the area, occupied by the seawall, of an intertidal zone or a sea area is reduced.

Подробнее
Номер записи: 1
26-05-2023 дата публикации

Method for enhancing photolyase activity through site-directed mutagenesis

Номер: CN116162613A
Автор: LYU JUN, SUI LONGFEI, CHEN YUANYUAN, SHEN SHUMIN, YAO CHENGKUN, JI ZIFAN, LUO FANGXIN, FU XIANGXIANG, XU LEI
Принадлежит:

The invention discloses a method for enhancing photolyase activity through site-specific mutagenesis, which comprises the following steps: carrying out site-specific mutagenesis on the site 345th for coding an escherichia coli photolyase gene phr, replacing the site for coding M345 of the escherichia coli photolyase gene phr with a codon for coding a Q residue, and connecting plasmid to form a connection product; converting a connection product into a DH5alpha strain; screening out transformed bacteria conforming to expectation; wherein the Q is glutamine. According to the invention, enhancement of CPD photolyase activity is realized, on the basis of Escherichia coli photolyase, a mutant with an M345 site replaced by a Q residue is constructed, the catalytic efficiency of the enzyme to a substrate is increased, the activity of the enzyme is stronger than that of an Escherichia coli photolyase wild type and other mutants of the M345 site, and a new scheme for enhancing the enzyme activity ...

Подробнее
Номер записи: 2