Systems, Methods And Composition of Using RNASE III Mutants to Produce sRNA to Control Host Pathogen Infection

The current invention includes systems, methods and compositions for the generation of sRNA molecules using select RNase III mutants. In one preferred embodiment, invention includes systems, methods and compositions for the generation of sRNA molecules using RNase III mutants to control a host pathogen through the production and diffusion of sRNA molecules that may initiate an RNAi pathway response directed to a host pathogen. Additional embodiments of the current invention include systems, methods and compositions for the DICER-independent generation of sRNA molecules using select RNase III mutants. 194-. (canceled)96. The genetically modified cell of wherein said genetically modified cell is selected from the group consisting of: a genetically modified prokaryotic cell claim 95 , and a genetically modified eukaryotic cell.97. The genetically modified cell of wherein said heterologous polynucleotide sequence operably linked to a promoter sequence encoding an RNase III mutant comprises at least one of the following:a heterologous polynucleotide sequence operably linked to a promoter sequence encoding an E38A RNase III mutant according SEQ ID NO. 3;a heterologous polynucleotide sequence operably linked to a promoter sequence encoding an E38A RNase III mutant according SEQ ID NOs. 9, and 13;a heterologous polynucleotide sequence operably linked to a promoter sequence encoding an E38A RNase III mutant according SEQ ID NO. 13;a heterologous polynucleotide sequence operably linked to a promoter sequence encoding an E65A RNase III mutant according to SEQ ID NO. 5;a heterologous polynucleotide sequence operably linked to a promoter sequence encoding an E38A-E65A RNase III mutant polynucleotide sequence encoding an amino acid sequence according to SEQ ID NO. 17;a heterologous polynucleotide sequence operably linked to a promoter sequence encoding an E38A-R107A-R108A RNase III mutant according to SEQ ID NO. 7;a heterologous polynucleotide sequence operably linked to a ...

Compositions And Methods For Glycosylating Cannabinoid Compounds

The present invention relates generally to the use of novel UDP-glucosyltransferases enzymes having specific activity towards cannabinoid compounds. The present invention further relates generally to the use of novel UGT enzymes having specific activity towards cannabinoid compounds to generate water-soluble cannabinoid glycoside compounds. 1. A method for producing select water-soluble cannabinoid compounds comprising the step of:providing a yeast cell expressing a heterologous nucleotide sequence, operably linked to a promoter, encoding a UDP-glucosyltransferases (UGT) having glycosylation activity towards a cannabinoid having at least one glycosylation site selected from the group consisting of: cannabidiol (CBD), cannabidiolic acid (CBDA), delta-9-tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCA); cannabigerol (CBG), and cannabigerolic acid (CBGA);introducing said cannabinoid having at least one glycosylation site to said yeast cell; andglycosylating said cannabinoid forming a cannabinoid glycoside.2. The method of claim 1 , wherein said step of introducing comprises the step of introducing said cannabinoid having at least one glycosylation site to a yeast cell culture.3. The method of claim 1 , wherein said UGT comprises a UGT selected from the group consisting of: SEQ ID NOs. 1-9181.4Pichia pastorisSaccharomyces cerevisiaeKluyveromyces marxianus. The method of claim 1 , wherein said yeast cell comprises a yeast cell selected from the group consisting of: a cell claim 1 , a cell claim 1 , and a cell.5. The method of claim 1 , wherein said nucleotide sequence encoding a heterologous UGT is codon optimized for expression in a yeast cell.6. A method for producing water-soluble cannabinoid compounds comprising:providing a yeast cell expressing a heterologous nucleotide sequence, operably linked to a promoter, encoding a UDP-glucosyltransferases (UGT) having glycosylation activity towards a cannabinoid;introducing said cannabinoid having at least one ...

Systems, methods and composition of using RNase III mutants to produce sRNA to control host pathogen infection

The current invention includes systems, methods and compositions for the generation of sRNA molecules using select RNase III mutants. In one preferred embodiment, invention includes systems, methods and compositions for the generation of sRNA molecules using RNase III mutants to control a host pathogen through the production and diffusion of sRNA molecules that may initiate an RNAi pathway response directed to a host pathogen. Additional embodiments of the current invention include systems, methods and compositions for the DICER-independent generation of sRNA molecules using select RNase III mutants.

Novel systems, methods, and compositions for the glycosylation of cannabinoid compounds

Systems, methods, and compositions for the generation of water-soluble cannabinoids utilizing fatty-acid binding proteins and fatty-acid binding-like proteins as cannabinoid-carriers

Sistemas, métodos y composiciones novedosas para la glucosilación de compuestos cannabinoides.

La presente invención se refiere generalmente a la identificación de enzimas UDP glucosiltransferasas novedosas que tienen actividad específica hacia compuestos cannabinoides. La presente invención además se refiere generalmente al uso de enzimas UGT novedosas que tienen actividad específica hacia compuestos cannabinoides para generar compuestos glucósidos cannabinoides hidrosolubles.

Systems, methods and composition of using rnase iii mutants to produce srna to control host pathogen infection

The current invention includes systems, methods and compositions for the generation of sRNA molecules using select RNase III mutants. In one preferred embodiment, invention includes systems, methods and compositions for the generation of sRNA molecules using RNase III mutants to control a host pathogen through the production and diffusion of sRNA molecules that may initiate an RNAi pathway response directed to a host pathogen. Additional embodiments of the current invention include systems, methods and compositions for the DICER-independent generation of sRNA molecules using select RNase III mutants.

Novel systems, methods, and compositions for the glycosylation of cannabinoid compounds

The present invention relates generally to the identification novel UDP- glucosyltransferases enzymes having specific activity towards cannabinoid compounds. The present invention further relates generally to the use of novel UGT enzymes having specific activity towards cannabinoid compounds to generate water-soluble cannabinoid glycoside compounds.

Systems, methods, and compositions for the generation of water-soluble cannabinoids utilizing fatty-acid binding proteins and fatty-acid binding-like proteins as cannabinoid-carriers

The inventive technology includes novel systems, methods, and compositions for the generation of water-soluble short-chain fatty acid phenolic compounds, preferably cannabinoids, terpenes, and other volatile compounds produced in Cannabis and other cannabinoid producing plants. In particular, the inventive technology includes novel systems and methods to solubilize short-chain fatty acid phenolic compounds, such as cannabinoids, via binding to a water soluble and readily digested carrier protein.

Systems, methods, and compositions for the generation of water-soluble cannabinoids utilizing fatty-acid binding proteins and fatty-acid binding-like proteins as cannabinoid-carriers

The inventive technology includes novel systems, methods, and compositions for the generation of water-soluble short-chain fatty acid phenolic compounds, preferably cannabinoids, terpenes, and other volatile compounds produced in Cannabis and other cannabinoid producing plants. In particular, the inventive technology includes novel systems and methods to solubilize short-chain fatty acid phenolic compounds, such as cannabinoids, via binding to a water soluble and readily digested carrier protein.