29-07-2021 дата публикации
Номер: US20210230552A1
Принадлежит:
The present invention includes a polypeptide for use in a three dimensional (3D) culture system for the growth of cells comprising one or more repeats of a sequence n-(XXGXP)-n(SEQ ID NO:8), wherein Xand Xare any amino acids except proline, wherein Xand X2 can be the same or different amino acid in solution or coated on a substrate, wherein nand nare equal to or greater than one, and wherein X is an aliphatic amino acid. 1. A polypeptide for use in a three dimensional (3D) culture system for the growth of cells comprising:{'sub': 1', '1', '2', '2', '1', '2', '1', '2', '1', '2, 'one or more repeats of a sequence n-(XXGXP)-n(SEQ ID NO:8), wherein X, X, Xare any amino acid, wherein X, X, and Xcan be the same or different amino acid, wherein nand nare equal to or greater than one.'}2. The polypeptide of claim 1 , the polypeptide has the sequence selected from at least one of [(XXGXP)(XXGXP)] claim 1 , (SEQ ID NO:68) [(XXGXP)(XXGXP)] (SEQ ID NO:69); (12) [(XXGXP)(XXGXP)(XXGXP)] (SEQ ID NO:70) claim 1 , wherein X claim 1 , X claim 1 , X claim 1 , X claim 1 , and Xare any amino acid claim 1 , and nand nare greater than or equal to one; wherein Xand Xcan be the same or different from each other claim 1 , and Xand Xcan be the same or different from each other; or wherein at least one of Xor Xis different from Xor X claim 1 , or wherein X is valine claim 1 , or X=G claim 1 , X=Y and A (in 1:4 ratio) and X=V.3. The polypeptide of claim 1 , further comprising attaching to claim 1 , or forming a fusion protein with claim 1 , the polypeptide and at least a portion of an extracellular matrix component selected from at least one of: glycosaminoglycans (GAGs) claim 1 , proteoglycans claim 1 , and/or proteins such as but not limited to laminin claim 1 , fibronectin claim 1 , vitronectin claim 1 , collagen claim 1 , elastin claim 1 , fibrillin claim 1 , fibulin claim 1 , tenascin claim 1 , perlecan claim 1 , versican claim 1 , aggrecan claim 1 , neurocan claim 1 , brevican claim 1 , ...
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