MODIFIED GENE RESULTING IN PARTHENOCARPIC FRUIT SET

The invention relates to a modified PIN4 gene, the wild type of which is as identified in SEQ ID No. 1, encoding the protein of SEQ ID No. 5, or the wild type of which encodes a protein that has a sequence similarity of at least 80% to SEQ ID No. 5, which modified PIN4 gene encodes a protein that comprises an amino acid change as a result of the modification, and which modified protein is capable of inducing parthenocarpic fruit set when present in a plant. The invention also relates to plants and fruits that carry the gene. Such plants are capable of parthenocarpic fruit set and the fruits do not contain seeds. The invention further relates to use of the gene in breeding and producing plants capable of parthenocarpic fruit set. 1. A modified PIN4 gene , the wild type of which is as identified in SEQ ID No. 1 , encoding the protein of SEQ ID No. 5 , or the wild type of which encodes a protein that has a sequence similarity of at least 80% to SEQ ID No. 5 , which modified PIN4 gene encodes a protein that comprises an amino acid change as a result of the modification , and which modified protein is capable of inducing parthenocarpic fruit set when present in a plant.2. The modified PIN4 gene as claimed in claim 1 , the wild type of which encodes a protein as identified in SEQ ID No. 5 claim 1 , or SEQ ID No. 12 claim 1 , or SEQ ID No. 13 claim 1 , or SEQ ID No. 14 claim 1 , or SEQ ID No. 15 claim 1 , or SEQ ID No. 16 claim 1 , which modified gene encodes a modified protein that comprises an amino acid change in the intracellular loop of the protein structure as a result of the modification claim 1 , which altered protein is capable of inducing parthenocarpic fruit set when present in a plant.3. The modified PIN4 gene as claimed in claim 1 , wherein the amino acid change of the protein results in a change in the structure of the protein claim 1 , which change in structure leads to the capability of parthenocarpic fruit set when the protein is present in a plant.4. The ...

GENETIC BASIS FOR CUCUMBER FRUIT HAVING SMALL SEED CAVITY

The present invention relates to a parthenocarpic cucumber fruit (L.) having a small seed cavity. A parthenocarpic cucumber fruit (L.) having a small seed cavity has a lower total moisture content. Two QTLs have been identified that either alone or in combination lead to the phenotype of having a small seed cavity. The invention further relates to a cucumber plant comprising one of the QTLs or a combination of both QTLs, which cucumber plant is capable of producing parthenocarpic fruits that have a small seed cavity. The invention also relates to markers for identifying the QTLs. 1. A parthenocarpic cucumber fruit which carries a QTL that leads to a small seed cavity in a parthenocarpic fruit.2. The parthenocarpic cucumber fruit as claimed in claim 1 , wherein the QTL is a QTL1 claim 1 , which QTL1 is located on chromosome 1 between marker sequences SEQ ID No. 1 and SEQ ID No. 2 claim 1 , preferably between marker sequences SEQ ID No. 9 and SEQ ID No. 2.3. The parthenocarpic cucumber fruit as claimed in claim 1 , wherein the QTL is a QTL1 claim 1 , which QTL1 is as comprised in the genome of a cucumber plant representative seed of which was deposited with the NCIMB under deposit numbers NCIMB 42411 and NCIMB 42424 and NCIMB 42590 claim 1 , and is in particular located therein between marker sequences SEQ ID No. 1 and SEQ ID No. 2 claim 1 , more particular between marker sequences SEQ ID No. 9 and SEQ ID No. 2.4. The parthenocarpic cucumber fruit as claimed in claim 1 , wherein the QTL is a QTL1 claim 1 , the presence of which QTL1 can be identified by any of the markers on chromosome 1 having SEQ ID No. 3 claim 1 , SEQ ID No. 4 claim 1 , SEQ ID No. 5 claim 1 , SEQ ID No. 6 claim 1 , SEQ ID No. 7 claim 1 , SEQ ID No. 8 claim 1 , SEQ ID No. 9 claim 1 , SEQ ID No. 10 claim 1 , SEQ ID No. 11 claim 1 , SEQ ID No. 12 claim 1 , SEQ ID No. 13 claim 1 , SEQ ID No. 14 claim 1 , and/or SEQ ID No. 15 claim 1 , preferably by any of the markers on chromosome 1 having SEQ ID No. ...

CYSDV RESISTANCE IN MEMBERS OF THE CUCURBITACEAE FAMILY

The present invention relates to a modified gene that provides resistance to Cucurbit Yellow Stunting Disorder virus (CYSDV) when present in a plant of the Cucurbitaceae family. The invention further relates to progeny, seed, and fruit of the plant that is resistant against CYSDV. The invention also relates to propagation material suitable for producing the plant that is resistant to CYSDV. Additionally, the invention relates to methods for producing, identifying, and selecting a plant of the Cucurbitaceae family having resistance against CYSDV. 1. A modified CLAPR1 gene , wherein the modified CLAPR1 gene encodes a modified protein comprising one or more modifications in the wild type protein sequence of SEQ ID NO: 2 or in a protein sequence having at least 86% sequence identity to SEQ ID NO: 2 , preferably 87% , 88% , 89% , 90% , 91% , 92% , 93% , 94% , 95% , 96% , 97% , 98% , or 99% sequence identity.2. The modified CLAPR1 gene as claimed in claim 1 , wherein the one or more modifications is selected from an amino acid substitution claim 1 , a premature stop codon claim 1 , or an insertion or deletion of one or more amino acids.3. The modified CLAPR1 gene as claimed in claim 1 , wherein the modified CLAPR1gene encodes a modified protein that comprises an insertion of at least one glutamine at position p. 533_534insQ of SEQ ID NO: 2.4. The modified CLAPR1 gene as claimed in claim 1 , wherein the modified gene encodes a modified protein that comprises three glutamine insertions at position p.533_534insQQQ in SEQ ID. No. 2.5. The modified CLAPR1 gene as claimed in claim 1 , wherein the modified gene encodes a modified protein that comprises one or more amino acid changes or a premature stop codon in its ENTH/VHS domain claim 1 , ENTH domain claim 1 , ANTH domain or Phosphoinositide binding clathrin adaptor domain 2.6. The modified CLAPR1 gene as claimed in claim 1 , wherein the modified protein as a result of the one or more modifications claim 1 , provides ...

GENETIC BASIS FOR PYTHIUM RESISTANCE

The present invention relates to a plant which may comprise a QTL, a copy number variant region, at least two copies of an ERF gene, or a mutation leading to increased expression of an ERF gene, which leads to resistance. The invention further relates to propagation material suitable for producing such plant. The invention also relates to a method for producing such plant and to methods for identification and selection of such a plant. In addition, the invention relates to a marker for identification of the QTL or copy number variant region, or for identification of the presence of at least two copies of an ERF gene resulting in resistance in , and to use of said marker. The invention also relates to seed which may comprise the QTL, copy number variant region, at least two copies of an ERF gene, or a mutation leading to increased expression of an ERF gene, which leads to resistance in the plant grown from such seed. 1Cucumis sativusPythium.. A plant comprising a QTL on chromosome 3 between SEQ ID NO: 1 and SEQ ID NO: 2 , the presence of which QTL leads to resistance against2Cucumis sativusPythium. A plant as claimed in claim 1 , wherein the resistance is due to the presence of a copy number variant region within the QTL claim 1 , which copy number variant region is flanked by SEQ ID NO: 4 and SEQ ID NO: 5.3Cucumis sativus. A plant as claimed in claim 2 , wherein the presence of the copy number variant region can be identified by determining the presence of at least one of the markers selected from the group consisting of SEQ ID NO: 4 claim 2 , SEQ ID NO: 5 claim 2 , SEQ ID NO: 6 claim 2 , SEQ ID NO: 7 claim 2 , SEQ ID NO: 8 claim 2 , SEQ ID NO: 9 claim 2 , SEQ ID NO: 10 claim 2 , SEQ ID NO: 11 claim 2 , SEQ ID NO:12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, and SEQ ID NO: 16.4Cucumis sativusPythium. A plant as claimed in claim 2 , wherein the resistance is due to the presence of at least two copies of an ERF gene within the copy number variant region claim 2 , ...

Qtl contributing to whitefly resistance in cucumis melo

The present invention relates to a Cucumis melo plant which carries QTL1 in its genome that leads to resistance against the Bemisia tabaci species complex, which QTL1 is located between flanking marker sequences SEQ ID No. 1 and SEQ ID No. 2 and can be identified by and is in particular linked to one or more markers selected from the group consisting of SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, and SEQ ID No. 12, or combinations thereof. In a further embodiment, the Cucumis melo plant further comprises another Bemisia resistance conferring QTL, which combination of QTLs leads to an improved level of resistance to the Bemisia tabaci species complex when compared to a plant in which only the other QTL is present.

Qtls for fusarium resistance in cucumber

The present invention relates to a cucumber plant which carries a QTL1 in its genome that leads to resistance to Fusarium oxysporum f. sp. radicis-cucumerinum. Said QTL1 is located on chromosome 3 between marker sequences SEQ ID No. 1 and SEQ ID No. 2 and can be identified by any of the markers having SEQ ID No. 3, and/or SEQ ID No. 4. QTL1 is as comprised in the genome of a cucumber plant representative seed of which was deposited with the NCIMB under deposit numbers NCIMB 42438 and NCIMB 42439. Optionally, in addition to the QTL1 also a QTL2 is present, which is located on chromosome 5 between marker sequences SEQ ID No. 5 and SEQ ID No. 6 and which can be identified by any of the markers on chromosome 5 having SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, and/or SEQ ID No. 10, or any combination of these SEQ ID Nos. QTL2 is as comprised in the genome of a cucumber plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 42439. A cucumber plant of the invention can comprise both QTLs, each either in homozygous or heterozygous form. The invention further relates to progeny of the plant, propagation material for the plant and to markers for identifying the QTLs and their use.

Modified gene resulting in parthenocarpic fruit set

Modified PIN4 gene, the wild type of which is as identified in SEQ ID No. 1, encoding the protein of SEQ ID No. 5, or the wild type of which encodes a protein that has a sequence similarity of at least 80% to SEQ ID No. 5, which modified PIN4 gene encodes a protein that comprises an amino acid change as a result of the modification, and which modified protein is capable of inducing parthenocarpic fruit set when present in a plant. The invention also relates to plants and fruits that carry the gene. Such plants are capable of parthenocarpic fruit set and the fruits do not contain seeds. The invention further relates to use of the gene in breeding and producing plants capable of parthenocarpic fruit set.

Genetic basis for cucumber fruit having small seed cavity

The present invention relates to a parthenocarpic cucumber fruit (Cucumis sativus L.) having a small seed cavity. A parthenocarpic cucumber fruit (Cucumis sativus L.) having a small seed cavity has a lower total moisture content. Two QTLs have been identified that either alone or in combination lead to the phenotype of having a small seed cavity. The invention further relates to a cucumber plant comprising one of the QTLs or a combination of both QTLs, which cucumber plant is capable of producing parthenocarpic fruits that have a small seed cavity. The invention also relates to markers for identifying the QTLs.

QTLs FOR FUSARIUM RESISTANCE IN CUCUMBER.

La presente invención se refiere a una planta de pepino que lleva un QTL1 en su genoma que conduce a proporcionar resistencia a Fusarium oxysporum f. sp. radicis-cucumerinum. Tal QTL1 está localizado en el cromosoma 3 entre las secuencias marcadoras de la SEC ID No. 1 y la SEC ID No.2 y puede identificarse por cualquiera de los marcadores que tienen la SEC ID No. 3, y/o la SEC ID No. 4. QTL1 está comprendido en el genoma de una semilla de la planta de pepino representativa, del cual se depositó ante el NCIMB bajo los números de depósito NCIMB 42438 y NCIMB 42439. Opcionalmente, además del QTL1, también está presente un QTL2, que se localiza en el cromosoma 5 entre las secuencias marcadoras de la SEC ID No. 5 y la SEC ID No. 6 y que pueden identificarse por cualquiera de los marcadores en el cromosoma 5 que tiene la SEC ID No. 7, la SEC ID No. 8, la SEC ID No. 9; y/o la SEC ID No. 10, o cualquier combinación de estos Nos. de SEC ID. QTL2 está comprendido en el genoma de una semilla representativa de una planta de pepino de la cual se depositó ante el NCIMB con el número de depósito NCIMB 42439. Una planta de pepino de la invención puede comprender ambos QTL, cada uno en forma homocigótica o heterocigótica. La invención se refiere además a la progenie de la planta, al material de propagación para la planta y a los marcadores para identificar los QTL y su uso.

Genetic basis for pythium resistance.

La presente invención se refiere a una planta Cucumis sativus que comprende un QTL, un número de copia de la región variante, al menos dos copias de un gen ERF, o una mutación que lleva a la expresión aumentada de un gen ERF, que lleva a la resistencia a Pythium. La invención además se refiere a material de propagación adecuado para producir tal planta Cucumis sativus. La invención también se refiere a un método para producir tal planta Cucumis sativus y a métodos para la identificación y selección de tal planta. Además, la invención se refiere a un marcador para la identificación del QTL o un número de copia de la región variante, o para la identificación de la presencia de al menos dos copias de un gen ERF que resultan en la resistencia a Pythium en Cucumis sativus, y al uso de tal marcador. La invención también se refiere una semilla que comprende el QTL, un número de copia de la región variante, al menos dos copias de un gen ERF, o una mutación que lleva a la expresión aumentada de un gen ERF, que lleva a la resistencia a Pythium en la planta cultivada de tal semilla.

QTLs FOR MCLCV RESISTANCE IN C. MELO

The invention relates to a

QTLs FOR MCLCV RESISTANCE IN C. MELO

The invention relates to a Cucumis melo plant that is resistant to MCLCV, which plant comprises a QTL on chromosome 11, and/or a QTL on chromosome 2, and/or a QTL on chromosome 6. The presence of the QTL on chromosome 11 can be identified by use of at least one of the markers of a group comprising SEQ ID Nos. 1, 5, and 2 to 4, and 17 to 21; the presence of the QTL on chromosome 2 can be identified by use of at least one of the markers of a group comprising SEQ ID Nos. 6, 12, and 7 to 11, and 22 to 27; and the presence of the QTL on chromosome 6 can be identified by use of at least one of the markers of a group comprising SEQ ID Nos. 13, 16, and 14 and 15, and 28 to 36. The QTL is as comprised in the genome of a Cucumis melo plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 43711, NCIMB 43712, NCIMB 43713, or NCIMB 43951.

Qtls for mclcv resistance in c. melo

The invention relates to a Cucumis melo plant that is resistant to MCLCV, which plant comprises a QTL on chromosome 11, and/or a QTL on chromosome 2, and/or a QTL on chromosome 6. The presence of the QTL on chromosome 11 can be identified by use of at least one of the markers of a group comprising SEQ ID Nos. 1, 5, and 2 to 4, and 17 to 21; the presence of the QTL on chromosome 2 can be identified by use of at least one of the markers of a group comprising SEQ ID Nos. 6, 12, and 7 to 11, and 22 to 27; and the presence of the QTL on chromosome 6 can be identified by use of at least one of the markers of a group comprising SEQ ID Nos. 13, 16, and 14 and 15, and 28 to 36. The QTL is as comprised in the genome of a Cucumis melo plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 43711, NCIMB 43712, NCIMB 43713, or NCIMB 43951.

Qtls for mclcv resistance in c. melo

The invention relates to a Cucumis melo plant that is resistant to MCLCV, which plant comprises a QTL on chromosome 11, and/or a QTL on chromosome 2, and/or a QTL on chromosome 6. The QTL is as comprised in the genome of a Cucumis melo plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 43711, NCIMB 43712, NCIMB 43713, or NCIMB 43951.

Genetic basis for pythium resistance

The present invention relates to a Cucumis sativus plant which comprises a copy number variant region which leads to Pythium and Didymella brioniae resistance. The invention further relates to propagation material suitable for producing such Cucumis sativus plant. The invention also relates to a method for producing such Cucumis sativus plant and to methods for identification and selection of such a plant. In addition, the invention relates to a marker for identification of the copy number variant region resulting in Pythium and Didymella brioniae resistance in Cucumis sativus, and to use of said marker. The invention also relates to seed which comprises the copy number variant region which leads to Pythium and Didymella brioniae resistance in the plant grown from such seed.

Locus de caracter cuantitativo (qtl) que contribuye a la resistencia a la mosca blanca en cucumis melo.

La presente invención se relaciona a una planta de Cucumis melo la cual porta QTL1 en su genoma que lleva a resistencia contra el complejo de especies de Bemisia tabaci, QTL1 que se ubica entre las secuencias de marcador de flanqueo SEQ ID NO. 1 y SEQ ID NO.2 y puede ser identificado por y está en particular enlazado a uno o más marcadores seleccionados del grupo que consiste de SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO. 11 y SEQ ID NO. 12 o una combinación de las mismas. En una modalidad adicional, la planta de Cucumis melo además comprende otro QTL que confiere resistencia a Bemisia, la combinación de QTL lleva a un nivel mejorado de resistencia al complejo de especies de Bemisia tabaci cuando se compara con una planta en la cual está presente solamente el otro QTL.

Gen modificado que da como resultado el cuajado de frutos partenocárpico

Gen PIN4 modificado, cuyo tipo salvaje es el identificado en SEQ ID No. 1, que codifica la proteína de SEQ ID No. 5, o cuyo tipo salvaje codifica una proteína que tiene una similitud de secuencia de al menos el 80% con SEQ ID No. 5, cuyo gen PIN4 modificado codifica una proteína que comprende un cambio de aminoácido como resultado de la modificación, y cuya proteína modificada es capaz de inducir el cuajado de frutos partenocárpicos cuando está presente en una planta. La invención también se refiere a plantas y frutos que portan el gen. Estas plantas son capaces de producir frutos partenocárpicos y los frutos no contienen semillas. La invención se refiere además al uso del gen en el mejoramiento y producción de plantas capaces de producir frutos partenocárpicos. (Traducción automática con Google Translate, sin valor legal)

QTL contributing to whitefly resistance in cucumis melo

The present invention relates to a

Locus de carácter cuantitativo (qtl) que contribuye a la resistencia a la mosca en cucumis melo

La presente invención se relaciona a una planta de Cucumis melo la cual porta QTL1 en su genoma que lleva a resistencia contra el complejo de especies de Bemisia tabaci, QTL1 que se ubica entre las secuencias de marcador de flanqueo SEQ ID NO. 1 y SEQ ID NO.2 y puede ser identificado por y está en particular enlazado a uno o más marcadores seleccionados del grupo que consiste de SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO. 11 y SEQ ID NO. 12 o una combinación de las mismas. En una modalidad adicional, la planta de Cucumis melo además comprende otro QTL que confiere resistencia a Bemisia, la combinación de QTL lleva a un nivel mejorado de resistencia al complejo de especies de Bemisia tabaci cuando se compara con una planta en la cual está presente solamente el otro QTL.

Qtl contributing to whitefly resistance in cucumis melo

The present invention relates to a Cucumis melo plant which carries QTL1 in its genome that leads to resistance against the Bemisia tabaci species complex, which QTL1 is located between flanking marker sequences SEQ ID No. 1 and SEQ ID No. 2 and can be identified by and is in particular linked to one or more markers selected from the group consisting of SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, and SEQ ID No. 12, or combinations thereof. In a further embodiment, the Cucumis mel o plant further comprises another Bemisia resistance conferring QTL, which combination of QTLs leads to an improved level of resistance to the Bemisia tabaci species complex when compared to a plant in which only the other QTL is present.

Modified gene resulting in parthenocarpic fruit set

Qtl contributing to whitefly resistance in cucumis melo

The present invention relates to a Cucumis melo plant which carries QTL1 in its genome that leads to resistance against the Bemisia tabaci species complex, which QTL1 is located between flanking marker sequences SEQ ID No. 1 and SEQ ID No. 2 and can be identified by and is in particular linked to one or more markers selected from the group consisting of SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, and SEQ ID No. 12, or combinations thereof. In a further embodiment, the Cucumis mel o plant further comprises another Bemisia resistance conferring QTL, which combination of QTLs leads to an improved level of resistance to the Bemisia tabaci species complex when compared to a plant in which only the other QTL is present.

Genetic basis for cucumber fruit having small seed cavity

Base genetica para resistencia a pythium.

La presente invención se refiere a una planta Cucumis sativus que comprende un QTL, un número de copia de la región variante, al menos dos copias de un gen ERF, o una mutación que lleva a la expresión aumentada de un gen ERF, que lleva a la resistencia a Pythium. La invención además se refiere a material de propagación adecuado para producir tal planta Cucumis sativus. La invención también se refiere a un método para producir tal planta Cucumis sativus y a métodos para la identificación y selección de tal planta. Además, la invención se refiere a un marcador para la identificación del QTL o un número de copia de la región variante, o para la identificación de la presencia de al menos dos copias de un gen ERF que resultan en la resistencia a Pythium en Cucumis sativus, y al uso de tal marcador. La invención también se refiere una semilla que comprende el QTL, un número de copia de la región variante, al menos dos copias de un gen ERF, o una mutación que lleva a la expresión aumentada de un gen ERF, que lleva a la resistencia a Pythium en la planta cultivada de tal semilla.