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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 4. Отображено 4.
01-01-2014 дата публикации

Method and kit for dual fluorescence quantitative PCR detection of grass carp reovirus types I and II

Номер: CN103484568A
Автор: Zeng Weiwei, Wang Qing, Wu Shuqin, Wang Yingying, Liu Chun, Liang Hongru, Shi Cunbin, Li Yingying
Принадлежит:

The invention discloses a method for dual fluorescence quantitative PCR detection of grass carp reovirus types I and II. The method can realize that the grass carp reovirus types I and II can be simultaneously detected in one to-be-detected sample, and the load levels of the two reovirus types can be quantified. The invention further provides a kit for dual fluorescence quantitative PCR detection of the grass carp reovirus types I and II. According to the detection method and the kit which are provided by the invention, the operation is simple and convenient, the specificity is strong, the detection sensitivity and accuracy rate are high and reliable, and a wide application prospect is achieved.

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Номер записи: 1
29-01-2014 дата публикации

Recombinant protein coded by grass carp reovirus (GCRV) type-II S10 gene, polyclonal antibody prepared from recombinant protein and application of recombinant protein

Номер: CN103539842A
Автор: Zeng Weiwei, Wang Qing, Wu Shuqin, Wang Yingying, Liang Hongru, Shi Cunbin, Li Yingying, Liu Chun
Принадлежит:

The invention discloses a recombinant protein coded by a grass carp reovirus (GCRV) type-II S10 gene, a polyclonal antibody prepared from the recombinant protein and an application of the recombinant protein. The amino acid sequence of the GCRV type-II S10 gene-coded protein is shown by SEQ ID No:2, and the nucleotide sequence coding the protein is shown by SEQ ID No:1. The recombinant protein disclosed by the invention has good immunogenicity; compared with the proteins of other structures of GCRV, the specific antibody valence generated by inducing an immune animal is higher. Further tests indicate that by immunizing the grass carp with the S10 recombinant protein, the grass carp can be induced to generate high specific antibody, and certain immune protection effect can be realized against the attack of a virulent strain of GCRV. Therefore, the study and application of the GCRV type-II S10 gene-coded protein are of vitally important significance to the development of a novel vaccine for ...

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Номер записи: 2
04-12-2013 дата публикации

Method for acquiring basin coverage rate for water conversation

Номер: CN103425869A
Автор: Yu Xinxiao, Zhao Yang, Jia Jianbo, Tu Zhihua, Wang Henian, Sun Fengbin, Sun Jiamei, Liang Hongru
Принадлежит:

The invention provides a method for acquiring a basin coverage rate for water conversation. The method includes the steps: 1) according to four stand types, including coniferous forest, broad-leaved forest, mixed broadleaf-conifer forest and shrubbery, of a basin forest, measuring area, canopy interception rate, saturated soil water-holding capacity and the like of each stand type; 2) superposing according to respective weights to acquire coverage area, capable of realizing water conservation, of one stand type, and determining a coverage rate of each stand for water conservation; 3) fitting to acquire a basin coverage rate used for water conservation. According to the method, the forest coverage rate of a basin for water conservation is obtained by combination of years' precipitation data of the studied basin according to forest area and forest types of a surface water basin, under the guidance of the coverage rate, reasonable planning and purposeful afforestation or deforestation are ...

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Номер записи: 3
11-12-2013 дата публикации

Construction method of bacterial ghost carrier

Номер: CN103436550A
Автор: Zhu Zhanbo, Jiang Dongjun, Yu Liquan, Cui Yudong, Wang He, Liang Hongru, Zhao Da, Hu Xu, Gao Jiabin, Chen Weihong, Yin Hui, Qiao Bo, Chen Nannan
Принадлежит:

The invention provides a construction method of a bacterial ghost carrier. The bacterial ghost carrier comprises anchor gene E', pasteurella OmpH gene, a lysate box (all sequences of pBV220-E from repressor proteins to terminators) and plasmid vector pET28a. The lysate box (ci857-E-T1T2) of the bacterial ghost carrier exists in a non-multiple cloning site zone of pET28a independently so as to form an expression system, the expression system and an original expression system of pET28a are independent with each other, and the directions of the two expression systems are opposite, so that it is convenient for insertion of target protein genes in a multiple cloning site zone of pET28a, conformation of the target protein is not influenced by protein expression, and false translation is difficult to occur.

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Номер записи: 4