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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 11. Отображено 9.
07-03-2017 дата публикации

Encapsulation and cardiac differentiation of hiPSCs in 3D PEG-fibrinogen hydrogels

Номер: US0009587221B2
Автор: Elizabeth A. Lipke, Petra Kerscher, Alexander J. Hodge, LIPKE ELIZABETH A, KERSCHER PETRA, HODGE ALEXANDER J, Lipke Elizabeth A., Kerscher Petra, Hodge Alexander J.
Принадлежит: Auburn University, UNIV AUBURN

The present invention relates to the production of cell cultures and tissues from undifferentiated pluripotent stem cells using three-dimensional biomimetic materials. The resultant cell cultures or tissues can be used in any of a number of protocols including testing chemicals, compounds, and drugs. Further, the methods and compositions of the present invention further provide viable cell sources and novel cell delivery platforms that allow for replacement of diseased tissue and engraftment of new cardiomyocytes from a readily available in vitro source. The present invention includes novel methods required for the successful production of cell cultures and tissues, systems and components used for the same, and methods of using the resultant cell and tissue compositions.

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Номер записи: 1
13-07-2017 дата публикации

ENCAPSULATION AND CARDIAC DIFFERENTIATION OF hiPSCs IN 3D PEG-FIBRINOGEN HYDROGELS

Номер: US20170198256A1
Автор: Elizabeth A. Lipke, Petra Kerscher, Alexander J. Hodge, LIPKE ELIZABETH A, KERSCHER PETRA, HODGE ALEXANDER J, Lipke Elizabeth A., Kerscher Petra, Hodge Alexander J.
Принадлежит:

The present invention relates to the production of cell cultures and tissues from undifferentiated pluripotent stem cells using three-dimensional biomimetic materials. The resultant cell cultures or tissues can be used in any of a number of protocols including testing chemicals, compounds, and drugs. Further, the methods and compositions of the present invention further provide viable cell sources and novel cell delivery platforms that allow for replacement of diseased tissue and engraftment of new cardiomyocytes from a readily available in vitro source. The present invention includes novel methods required for the successful production of cell cultures and tissues, systems and components used for the same, and methods of using the resultant cell and tissue compositions. 120-. (canceled)21. A method of producing a three-dimensional cardiac tissue comprising:combining a population of pluripotent stem cells (PSCs) with a hydrogel precursor solution to form a PSC suspension, the hydrogel precursor solution comprising an acrylate component and a natural hydrogel-forming component chosen from a naturally occurring protein, a naturally occurring polysaccharide, a naturally occurring protein derivative, or a naturally occurring polysaccharide derivative;treating the PSC suspension by cross-linking to produce a three-dimensional PSC microenvironment; andculturing the three-dimensional PSC microenvironment to differentiate the PSCs into a cardiac tissue.22. The method of claim 21 , wherein treating the PSC suspension further comprises placing the PSC suspension into a mold prior to cross-linking.23. The method of claim 21 , wherein the three-dimensional PSC microenvironment is selected from the group consisting of microislands claim 21 , cardiac discs claim 21 , strings claim 21 , macrotissues claim 21 , and microspheres claim 21 , and combinations thereof.24. The method of claim 21 , wherein treating the PSC suspension to produce a three-dimensional PSC microenvironment ...

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Номер записи: 2
15-12-2016 дата публикации

NOVEL PEPTIDES FOR SUPPORTING ENDOTHELIAL PROGENITOR CELL ROLLING AND CAPTURE AND ENDOTHELIALIZATION OF BIOMATERIALS

Номер: US20160361471A1
Автор: Elizabeth A. Lipke, Wen Jun Seeto, LIPKE ELIZABETH A, SEETO WEN JUN, Lipke Elizabeth A., Seeto Wen Jun
Принадлежит: AUBURN UNIVERSITY

The present invention relates to the production of endothelialized matrices and materials from immature endothelial cells using substrates to which particular peptides have been grafted. The resultant substrates can be used to capture and support immature endothelial cells. Further, the methods and compositions of the present invention provide viable cell delivery platforms that allow for production and provision of endothelialized medical devices and implants, including vascular grafts, stents, shunts, and valves, endothelialized surfaces and channels for in vitro testing devices, including microfluidic chips, and materials that support vascularization such as for use in engineered tissues. The present invention includes novel methods required for the successful production of cellularized substrates, systems and components used for the same, and methods of using the resultant cell and tissue compositions.

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Номер записи: 3
11-04-2019 дата публикации

Microfluidics device for fabrication of large, uniform, injectable hydrogel microparticles for cell encapsulation

Номер: US20190105279A1
Автор: Elizabeth A. Lipke, Wen Jun Seeto, Yuan Tian
Принадлежит: AUBURN UNIVERSITY

The devices, methods, and compositions disclosed herein accomplish robust cell encapsulation in polymer microparticles using a vertically oriented microfluidic device. A hydrophilic polymer precursor solution is flowed into a first inlet channel, which extends inward from an upper surface of the device housing. A hydrophobic fluid is flowed into a second inlet channel, which extends inward from a lower surface of the device housing. The two inlet channels meet at a junction, and an outlet channel extends away from the two inlet channels. When the two inwardly flowing streams meet at the junction, the polymer precursor solution disperses into the hydrophobic fluid. The dispersed precursor droplets are photopolymerized into microparticles as they travel through the outlet channel. The resulting microparticles are highly uniform, and are larger than conventionally formed microparticles. Cells of varying types can be encapsulated with high viability and spatial uniformity.

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Номер записи: 4
14-05-2015 дата публикации

ENCAPSULATION AND CARDIAC DIFFERENTIATION OF hiPSCs IN 3D PEG-FIBRINOGEN HYDROGELS

Номер: US20150132847A1
Автор: Hodge Alexander J., Kerscher Petra, Lipke Elizabeth A.
Принадлежит:

The present invention relates to the production of cell cultures and tissues from undifferentiated pluripotent stem cells using three-dimensional biomimetic materials. The resultant cell cultures or tissues can be used in any of a number of protocols including testing chemicals, compounds, and drugs. Further, the methods and compositions of the present invention further provide viable cell sources and novel cell delivery platforms that allow for replacement of diseased tissue and engraftment of new cardiomyocytes from a readily available in vitro source. The present invention includes novel methods required for the successful production of cell cultures and tissues, systems and components used for the same, and methods of using the resultant cell and tissue compositions. 1. A method of producing a three-dimensional cell culture or tissue comprising:combining a population of pluripotent stem cells (PSCs) with a biomimetic material to form a biomimetic-PSC suspension;treating said biomimetic-PSC suspension to produce a three-dimensional biomimetic-PSC microenvironment; andculturing said biomimetic-PSC microenvironment to differentiate the PSCs into at least one type of somatic cell.2. The method of wherein the biomimetic material is a hydrogel.3. The method of wherein the hydrogel is a covalently-linkable hydrogel.4. The method of wherein the covalently-linkable hydrogel is a PEG-based hydrogel.5. The method of wherein the PEG-based hydrogel comprises PEG-fibrinogen.6. The method of wherein said treating said biomimetic-PSC suspension further comprises placing said biomimetic-PSC suspension into a mold.7. The method of wherein said microenvironment is selected from the group consisting of microislands claim 1 , cardiac discs claim 1 , strings claim 1 , macrotissues claim 1 , and microspheres claim 1 , and combinations thereof.8. The method of wherein said treating said biomimetic-PSC suspension to produce a three-dimensional biomimetic-PSC microenvironment comprises ...

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Номер записи: 5
16-07-2020 дата публикации

Novel peptides for supporting endothelial progenitor cell rolling and capture and endothelialization of biomaterials

Номер: US20200222596A1
Автор: Elizabeth A. Lipke, Wen Jun Seeto
Принадлежит: AUBURN UNIVERSITY

The present invention relates to the production of endothelialized matrices and materials from immature endothelial cells using substrates to which particular peptides have been grafted. The resultant substrates can be used to capture and support immature endothelial cells. Further, the methods and compositions of the present invention provide viable cell delivery platforms that allow for production and provision of endothelialized medical devices and implants, including vascular grafts, stents, shunts, and valves, endothelialized surfaces and channels for in vitro testing devices, including microfluidic chips, and materials that support vascularization such as for use in engineered tissues. The present invention includes novel methods required for the successful production of cellularized substrates, systems and components used for the same, and methods of using the resultant cell and tissue compositions.

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Номер записи: 6
19-09-2019 дата публикации

ENCAPSULATION AND CARDIAC DIFFERENTIATION OF HIPSCS IN 3D PEG-FIBRINOGEN HYDROGELS

Номер: US20190284534A1
Автор: Hodge Alexander J., Kerscher Petra, Lipke Elizabeth A.
Принадлежит:

The present invention relates to the production of cell cultures and tissues from undifferentiated pluripotent stem cells using three-dimensional biomimetic materials. The resultant cell cultures or tissues can be used in any of a number of protocols including testing chemicals, compounds, and drugs. Further, the methods and compositions of the present invention further provide viable cell sources and novel cell delivery platforms that allow for replacement of diseased tissue and engraftment of new cardiomyocytes from a readily available in vitro source. The present invention includes novel methods required for the successful production of cell cultures and tissues, systems and components used for the same, and methods of using the resultant cell and tissue compositions. 120.-. (canceled)21. A three-dimensional , synchronously contracting cardiac tissue comprising;a hydrogel material comprising a covalently crosslinkable component and a natural hydrogel component, the natural hydrogel component comprising one or more of fibrinogen, collagen, gelatin, hyaluronic acid, elastin, fibronectin, laminin, fibrin, alginate, and decellularized cardiac extracellular matrix, andthree-dimensionally differentiated, PSC-derived cardiomyocytes encapsulated within the hydrogel material.22. The three-dimensional claim 21 , synchronously contracting cardiac tissue of claim 21 , wherein the synchronously contracting cardiac tissue contracts as a single unit.23. The three-dimensional claim 22 , synchronously contracting cardiac tissue of claim 22 , wherein the synchronously contracting cardiac tissue contracts spontaneously.24. The three-dimensional claim 23 , synchronously contracting cardiac tissue of claim 23 , wherein the synchronously contracting cardiac tissue contracts spontaneously without stimulation.25. The three-dimensional claim 23 , synchronously contracting cardiac tissue of claim 23 , wherein the spontaneous contraction frequency ranges from 0.59 to 1.53 Hertz.26. The ...

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Номер записи: 7
22-12-2022 дата публикации

ENCAPSULATION AND CARDIAC DIFFERENTIATION OF hiPSCs IN 3D PEG-FIBRINOGEN HYDROGELS

Номер: US20220403338A1
Автор: Hodge Alexander J., Kerscher Petra, Lipke Elizabeth A.
Принадлежит:

The present invention relates to the production of cell cultures and tissues from undifferentiated pluripotent stem cells using three-dimensional biomimetic materials. The resultant cell cultures or tissues can be used in any of a number of protocols including testing chemicals, compounds, and drugs. Further, the methods and compositions of the present invention further provide viable cell sources and novel cell delivery platforms that allow for replacement of diseased tissue and engraftment of new cardiomyocytes from a readily available in vitro source. The present invention includes novel methods required for the successful production of cell cultures and tissues, systems and components used for the same, and methods of using the resultant cell and tissue compositions. 120-. (canceled)21. A method of screening a candidate compound or substance for an effect on cardiac tissue , the method comprising:forming a biomimetic-PSC suspension by suspending a population of pluripotent stem cells (PSCs) in a covalently crosslinkable component and a natural hydrogel component, the natural hydrogel component comprising one or more of fibrinogen, collagen, gelatin, hyaluronic acid, elastin, fibronectin, laminin, fibrin, alginate, and decellularized cardiac extracellular matrix;crosslinking the biomimetic-PSC suspension to produce a three-dimensional biomimetic PSC microenvironment;culturing the three-dimensional biomimetic-PSC microenvironment to differentiate the three-dimensional biomimetic-PSC microenvironment into a three-dimensional, synchronously contracting cardiac tissue;exposing the cardiac tissue to a candidate compound or substance; anddetermining an effect of the candidate compound or substance on the cardiac tissue.22. The method of claim 21 , wherein determining an effect comprises determining a level of toxicity of the candidate compound or substance to the cardiac tissue.23. The method of claim 21 , wherein determining an effect comprises determining a level of ...

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Номер записи: 8
28-04-2022 дата публикации

Microfluidics device for fabrication of large, uniform, injectable hydrogel microparticles for cell encapsulation

Номер: US20220125735A1
Автор: Elizabeth A. Lipke, Wen Jun Seeto, Yuan Tian
Принадлежит: AUBURN UNIVERSITY

The devices, methods, and compositions disclosed herein accomplish robust cell encapsulation in polymer microparticles using a vertically oriented microfluidic device. A hydrophilic polymer precursor solution is flowed into a first inlet channel, which extends inward from an upper surface of the device housing. A hydrophobic fluid is flowed into a second inlet channel, which extends inward from a lower surface of the device housing. The two inlet channels meet at a junction, and an outlet channel extends away from the two inlet channels. When the two inwardly flowing streams meet at the junction, the polymer precursor solution disperses into the hydrophobic fluid. The dispersed precursor droplets are photopolymerized into microparticles as they travel through the outlet channel. The resulting microparticles are highly uniform, and are larger than conventionally formed microparticles. Cells of varying types can be encapsulated with high viability and spatial uniformity.

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Номер записи: 9