Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides

A porous material comprising a copolymer comprising at least on hydrophilic monomer and at least a first and second poly-amide bonded phase, wherein the average pore size is greater than or equal to 200 Å or between 1 and 50Å. The porous material may comprise porous particles or a porous monolith comprising said copolymer. The hydrophilic polymer preferably comprises monomers having di or tri alkoxy silanes. The poly-amide bonded phase may comprise acrylamides, acrylates, acrylonitriles, and preferably poly(divinyl benzene-co-N-vinylcaprolactam). The material can have a median pore diameter of about 100 Å to about 1000 Å and a nitrogen content from about 0.5% to about 20%. Methods using said porous material for isolating, analysing and determining levels of a component from a mixture are disclosed, particularly via hydrophilic interaction liquid chromatography (HILIC). Methods are directed towards the separation of proteins and glycosylated proteins, due to improved pore diffusion arising ...