PROCEDURE FOR THE DETERMINATION UNKNOWN QUANTITIES OF A PNS SEQENZ AND ITS USES

Mikrodissektionsgerät und Verfahren zum Isolieren von Zellen eines vorbestimmten Zelltyps

Die Erfindung betrifft ein Mikrodissektionsgerät (10) und ein Verfahren zum Isolieren von Zellen eines vorbestimmten Zelltyps aus mindestens einer vorbestimmten Gewebeprobe (18) auf jeweils einem Probenträger (16) in einem Mikrodissektionsgerät (10), umfassend die Schritte des Bereitstellens eines Bildes (30) von einer ersten Gewebeprobe (18) als Referenzprobe durch eine Bildaufnahmeeinrichtung (12, S3) und des Erzeugens eines Auswahlsignals durch eine Analyseeinrichtung (20, S4), das Koordinaten von mindestens einer Begrenzung eines Gewebeausschnitts (36) auf dem Probenträger (16) mit der Referenzprobe (18) beschreibt. Es erfolgt das Ausrichten mindestens eines Schneidelements (38) gemäß der mindestens einen Begrenzung mindestens einer vorbestimmten Gewebeprobe (18, S6) und hierdurch Entfernen des ausgewählten Gewebeausschnitts (36, S7). Eine Abtasteinrichtung (42) kann ein Abtasten der mindestens einen vorbestimmten Gewebeprobe (18) durchführen (S9) und dadurch eine Abweichung einer relativen ...

IProton Sponge As Supplement To Electrolytes For Photocatalytic And Electrochemical Co2 Reduction

The invention relates to a method for converting carbon dioxide and water, wherein the electrolyte comprises a proton sponge which serves to accumulate CO2 in the electrolyte. The invention further relates to a corresponding use of a proton sponge and to an electrolyte comprising at least one proton sponge.

Flexibles Extraktionsverfahren für die Herstellung sequenzspezifischer Molekülbibliotheken

Die Erfindung betrifft ein Extraktionsverfahren zur Isolierung von Zielmolekülen aus einer Probe unter Verwendung einer Molekülbibliothek.

FLEXIBLE EXTRACTION METHOD FOR THE PRODUCTION OF SEQUENCE-SPECIFIC MOLECULE LIBRARIES

The invention relates to an extraction method for isolating target molecules from a sample with the help of a molecule library.

Photobioreaktor für immobilisierte Mikroorganismen

Die Erfindung betrifft einen Photobioreaktor (10) mit einem Reaktionsbehälter (12) und eine in dem Reaktionsbehälter (12) angeordnete Rückhalteeinrichtung (14). Die Rückhalteeinrichtung (14) ist dazu ausgebildet, einen im Innern des Reaktionsbehälters (12) vorhandenen photosynthetischen Organismus (22) relativ zu einem den photosynthetischen Organismus (22) umgebenen Kulturmedium (21) festzuhalten. Die Rückhalteeinrichtung (14) kann dabei zumindest einen Lichtleiter (24) umfassen. Die Erfindung umfasst weiterhin ein Verfahren zum Betreiben eines Photobioreaktors (10) mit den Schritten des Bereitstellens mindestens eines photosynthetischen Organismus (22) in einem Reaktionsbehälter (12), des Fixierens des bereitgestellten Organismus (22) an eine in dem Reaktionsbehälter (12) angeordnete Rückhalteeinrichtung (14), die einen Lichtleiter (24) umfasst, und des Beaufschlagens der Rückhalteeinrichtung (14) mit Licht. Weiterhin bezieht sich die Erfindung auf ein entsprechendes Verfahren zum Herstellen ...

IProton sponge as supplement to electrolytes for photocatalytic and electrochemical Co2 reduction

The invention relates to a method for converting carbon dioxide and water, wherein the electrolyte comprises a proton sponge which serves to accumulate CO2 in the electrolyte. The invention further relates to a corresponding use of a proton sponge and to an electrolyte comprising at least one proton sponge.

Method for determining an unknown PNA sequence and uses thereof

The invention relates to a method for determining an unknown PNA sequence information of PNA molecules of a specific PNA molecule species, wherein, the PNA molecules are contacted with one or several different nucleic acid molecule species comprising nucleic acid molecules with at least one nucleotide, wherein the nucleic acid molecules at least partially comprise a nucleic acid sequence that is complementary to at least a partial sequence of the PNA molecule, wherein nucleic acid molecules having complementary sequences bind to the PNA molecules forming nucleic acid/PNA hybrids, wherein nucleic acid molecules with non-complementary sequences are separated from the nucleic acid/PNA hybrids, wherein thereafter the nucleic acid/PNA hybrids are dissociated into single stranded hybrid nucleic acid molecules and PNA molecules, wherein the single stranded hybrid nucleic acid molecules are subjected to a sequencing process providing hybrid sequence information about the single stranded hybrid nucleic acid sequence, and wherein the hybrid sequence information is optionally translated into the complementary PNA sequence information, and to a method for producing PNA molecules using such a process.

SYNTHESIS OF SEQUENCE-VERIFIED NUCLEIC ACIDS

The invention relates to methods and devices for preparing synthetic nucleic acids.

Selbst-separierende Mikroorganismen

Es wird ein Verfahren zum Ernten von Mikroorganismen aus einem flüssigen Medium beschrieben, das die Schritte Kultivierung der Mikroorganismen in dem Medium, Induzieren einer Bildung eines Lipids durch die Mikroorganismen, und Abschöpfen der Mikroorganismen von der Oberfläche des Mediums, umfasst.

Verfahren zum Kultivieren eines Mikroorganismus in einer in vitro Kultur und Verfahren zum Herstellen eines Sekundärmetabolits

Die Erfindung betrifft ein Verfahren zum Kultivieren eines Mikroorganismus (10), insbesondere eines photosynthetischen Mikroorganismus (10). Das Verfahren umfasst die Schritte des Bereitstellens des Mikroorganismus (10) und eines Kulturmediums (12) in einem Reaktionsbehälter (26) eines Bioreaktors (24), des Inkontaktbringens des Mikroorganismus (10) mit einer von einer Innenwand des Reaktionsbehälters (26) beabstandenden Oberfläche einer Rückhalteeinrichtung (14) (A) und des Bereitstellens eines Substrats (16) als Ausgangsstoff für den Stoffwechsel des Mikroorganismus (10, B). Der Mikroorganismus (10) weist die Eigenschaft auf, in einem lebenden Zustand an eine Oberfläche zu adhärieren und sich nach Absterben bei Vorliegen einer vorbestimmten Ablösebedingung wieder von der Oberfläche zu lösen. Weiterhin kann ein weiterer Mikroorganismus (10) mit der Oberfläche in Kontakt gebracht werden (D), z.B. durch Einstellen einer Bedingung, die das Vermehren des vorhandenen Mikroorganismus (10) auslöst ...

Molecular-biological processing unit for detection, analysis of genomes, for producing e.g. synthetic nucleic acids, comprises device for in-situ-synthesis of arrays of receptors

The molecular biological processing unit comprises a device for the in-situ-synthesis of arrays of receptors. One or more elements are provided for the eradication of fluidic steps, like probe addition, reagent addition, washing steps or probe conduction. A detection unit is provided for detecting an optical or electric signal. A memory programmed unit is provided for the control of the synthesis. A memory programmed unit is provided for the control of the fluidics, for the control of the detection and the storage and management of the measuring data. Independent claims are included for: (1) a method for the analysis of the nucleic acid sequence and amplification of a target nucleic acid; (2) a method for producing a carrier for regulating nucleic acid analyte by hybridization; (3) a method for regulating analyte; (4) a method for amplification of analyte; and (5) a reagent kit.

Protonenschwämme als Zusatz zu Elektrolyten für die photokatalytische und elektrochemische CO2-Reduktion

Die vorliegende Erfindung betrifft ein Verfahren zur Umsetzung von Kohlendioxid und Wasser, bei dem der Elektrolyt einen Protonenschwamm umfasst, der zur Anreicherung von CO2 im Elektrolyten dient, eine entsprechende Verwendung eines Protonenschwamms sowie einen Elektrolyten umfassend mindestens einen Protonenschwamm. The present invention relates to a process for the conversion of carbon dioxide and water, in which the electrolyte comprises a proton sponge, which serves for the accumulation of CO2 in the electrolyte, a corresponding use of a proton sponge and an electrolyte comprising at least one proton sponge.

Method for determining an unknown PNA sequence and uses thereof

The invention relates to a method for the sequence information of PNA molecules of a specific PNA molecule species, wherein, the PNA molecules are contacted with different nucleic acid molecule species comprising nucleic acid molecules with nucleotides, wherein the nucleic acid molecules partially comprise a nucleic acid sequence that is complementary to a partial sequence of the PNA molecule, wherein nucleic acid molecules having complementary sequences bind to the PNA molecules forming nucleic acid/PNA hybrids, wherein nucleic acid molecules with non-complementary sequences are separated from the hybrids, wherein thereafter the hybrids are dissociated into single stranded hybrid nucleic acid molecules and PNA molecules, wherein the single stranded hybrid nucleic acid molecules are subjected to a sequencing process providing hybrid sequence information about the single stranded hybrid nucleic acid sequence, and wherein the hybrid sequence information is optionally translated into the complementary PNA sequence information. 1. A method for determining an unknown PNA sequence information of PNA molecules of a specific PNA molecule species , comprising the following steps:a) contacting the PNA molecules with one or several different nucleic acid molecule species comprising nucleic acid molecules with at least one nucleotide, wherein the nucleic acid molecules at least partially comprise a nucleic acid sequence that is complementary to at least a partial sequence of the PNA molecule,b) binding the nucleic acid molecules having complementary sequences to the PNA molecules forming nucleic acid/PNA hybrids,c) separating the nucleic acid molecules with non-complementary sequences from the nucleic acid/PNA hybrids and/or are degraded enzymatically,d) dissociating the nucleic acid/PNA hybrids into single stranded hybrid nucleic acid molecules and PNA molecules,e) subjecting the single stranded hybrid nucleic acid molecules to a sequencing process providing hybrid sequence ...

Synthesis of sequence-verified nucleic acids

The invention relates to methods and devices for preparing synthetic nucleic acids.

AMPLIFICATION-INTEGRATED GENETIC MATERIAL DEPLETION OF NON-TARGET ORGANISMS USING DIFFERENTIALLY ABUNDANT K-MERS

The present invention relates to a method of selectively amplifying at least one nucleic acid sequence of at least one microorganism and/or virus in a sample of a subject, wherein k-mers 3 are applied that show a difference in frequency and/or context in the genome 2 of the at least one microorganism and/or virus compared to the genome of the subject 1.

SYNTHESIS OF SEQUENCE-VERIFIED NUCLEIC ACIDS

The invention relates to methods and devices for preparing synthetic nucleic acids.

MOLECULAR PREDICTORS OF PATIENT RESPONSE TO RADIOTHERAPY TREATMENT

Disclosed are methods of predicting a radiotherapy success in a method of treating a lung cancer of a patient, the use of specific markers for predicting a radiotherapy success in a method of treating a lung cancer of a patient, a database comprising the markers, and a computer program product for use in such a method. 1. A method of predicting a radiotherapy success in a method of treating a lung cancer of a patient , the method comprising:obtaining or providing a sample of the patient, anddetermining a change in at least one of a nucleotide sequence, epigenomics profile, expression profile or copy number compared to at least one of a reference sequence, epigenomics profile, expression profile or copy number of at least one of (i) at least one gene selected from MLH1, EP300, BRCA2, FLT1, GRINA, HEATR1, MUTYH, NUDT1, PARP10, PRPF19, KCNJ12, MGMT, PMS2P9, DDX11, EPHA2, GEN1, SETD2, and ZNF208, (ii) a gene mutational burden in at least one gene selected from the group consisting of MLH1, DDX11, APOLD1, GEN1, GPER1, POLB, PRPF19, ANKRD30A, AR, BTK, GTF2H4, HEATR1, IKBKE, KAT6A, NUDT19, PARP1, PTPRT, TAF3, TDG, MAP3K1, and SLC9A4, or (iii) a tumor mutational burden (TMB).2. The method of claim 1 , wherein the lung cancer is non-small cell lung cancer claim 1 , and wherein at least one ofthe change in the at least one of the nucleotide sequence, epigenomics profile, expression profile or copy number compared to the at least one of the reference sequence, epigenomics profile, expression profile or copy number, is determined in at least one gene selected from the group consisting of BRCA2, DDX11, EPHA2, FLT1, GEN1, GRINA, HEATR1, MLH1, MUTYH, NUDT1, PARP10, PRPF19, SETD2, ZNF208, EP300, KCNJ12, MGMT, and PMS2P9,the gene mutational burden is determined in at least one gene selected from the group consisting of APOLD1, DDX11, GEN1, GPER1, MLH1, POLB, PRPF19, ANKRD30A, AR, BTK, GTF2H4, HEATR1, IKBKE, KAT6A, MAP3K1, NUDT19, PARP1, PTPRT, SLC9A4, TAF3, and TDG,the tumor ...

A biochemical analytical technique

A biochemical analytical device and a biochemical analytical method for determining an analyte in a test sample are provided. In the technique, the biochemical analytical device includes a sample port to receive the test sample, a sensor to probe the test sample and to generate sensor data, and a processor. The sensor data corresponds to the analyte in the test sample. The processor receives the sensor data from the sensor and selects a non-linear function for the received sensor data. The processor fits the selected non-linear function to the sensor data. Additionally, the processor compares the fitted non-linear function to a reference data to determine the analyte in the test sample.

Amplification-integrated genetic material depletion of non-target organisms using differentially abundant k-mers

The present invention relates to a method of selectively amplifying at least one nucleic acid sequence of at least one microorganism and/or virus in a sample of a subject, wherein k-mers 3 are applied that show a difference in frequency and/or context in the genome 2 of the at least one microorganism and/or virus compared to the genome of the subject 1.

Proton sponge as supplement to electrolytes for photocatalytic and electrochemical co2 reduction

Die vorliegende Erfindung betrifft ein Verfahren zur Umsetzung von Kohlendioxid und Wasser, bei dem der Elektrolyt einen Protonenschwamm umfasst, der zur Anreicherung von CO 2 im Elektrolyten dient, eine entsprechende Verwendung eines Protonenschwamms sowie einen Elektrolyten umfassend mindestens einen Protonenschwamm.

Molecular predictors of patient response to radiotherapy treatment

The present invention relates to methods of predicting a radiotherapy success in a method of treating a lung cancer of a patient, the use of specific markers for predicting a radiotherapy success in a method of treating a lung cancer of a patient, a database comprising the markers, and a computer program product for use in such a method.

Method for determining an unknown PNA sequence and uses thereof

The invention relates to a method for determining an unknown PNA sequence information of PNA molecules of a specific PNA molecule species, wherein, the PNA molecules are contacted with one or several different nucleic acid molecule species comprising nucleic acid molecules with at least one nucleotide, wherein the nucleic acid molecules at least partially comprise a nucleic acid sequence that is complementary to at least a partial sequence of the PNA molecule, wherein nucleic acid molecules having complementary sequences bind to the PNA molecules forming nucleic acid/PNA hybrids, wherein nucleic acid molecules with non-complementary sequences are separated from the nucleic acid/PNA hybrids, wherein thereafter the nucleic acid/PNA hybrids are dissociated into single stranded hybrid nucleic acid molecules and PNA molecules, wherein the single stranded hybrid nucleic acid molecules are subjected to a sequencing process providing hybrid sequence information about the single stranded hybrid nucleic acid sequence, and wherein the hybrid sequence information is optionally translated into the complementary PNA sequence information, and to a method for producing PNA molecules using such a process.

Proton sponge as supplement to electrolytes for photocatalytic and electrochemical co2 reduction

The invention relates to a method for converting carbon dioxide and water, wherein the electrolyte comprises a proton sponge which serves to accumulate CO 2 in the electrolyte. The invention further relates to a corresponding use of a proton sponge and to an electrolyte comprising at least one proton sponge.

Method for determining an unknown PNA sequence and uses thereof

Verfahren zur bestimmung einer unbekannten pns seqenz und seine verwendungen

Amplification-integrated genetic material depletion of non-target organisms using differentially abundant k-mers

The present invention relates to a method of selectively amplifying at least one nucleic acid sequence of at least one microorganism and/or virus in a sample of a subject, wherein k-mers (3) are applied that show a difference in frequency and/or context in the genome (2) of the at least one microorganism and/or virus compared to the genome of the subject (1).

Amplification-integrated genetic material depletion of non-target organisms using differentially abundant k-mers

The present invention relates to a method of selectively amplifying at least one nucleic acid sequence of at least one microorganism and/or virus in a sample of a subject, wherein k-mers (3) are applied that show a difference in frequency and/or context in the genome (2) of the at least one microorganism and/or virus compared to the genome of the subject (1).

Amplification-integrated genetic material depletion of non-target organisms using differentially abundant k-mers

The present invention relates to a method of selectively amplifying at least one nucleic acid sequence of at least one microorganism and/or virus in a sample of a subject, wherein k-mers 3 are applied that show a difference in frequency and/or context in the genome 2 of the at least one microorganism and/or virus compared to the genome of the subject 1.