06-06-2023 дата публикации
Номер: CN116223650A
Принадлежит:
The invention belongs to the technical field of medicine detection, and particularly relates to a high performance liquid chromatography detection method of imidazole acyl chloride. According to the detection method, a high performance liquid chromatography is adopted, a C18 chromatographic column is adopted, the detection wavelength is 200-220 nm, the column temperature is high, an acetonitrile-10 mol/L potassium hydrogen phthalate buffer solution is used as a mobile phase, the flow velocity is 0.5-1.2 ml/min, and isocratic elution is performed; a sample is subjected to derivatization treatment before being detected, an amine compound is used as a derivatization reagent, an alkaline compound is used as a catalyst, absolute ethyl alcohol is used as a solvent, reaction is carried out at room temperature for 5-20 min, and derivatization treatment is completed. The detection method disclosed by the invention is convenient and simple, high in sensitivity, good in chromatographic peak shape, ...
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