08-05-2014 дата публикации
Номер: US20140128253A1
The present invention provides a porous cellulose gel having a high mechanical strength capable of being operated at a higher flow rate, and a method for producing the same. To a suspension liquid of cellulose particles, a crosslinking agent in an amount of from 4 to 12 times the amount of the cellulose monomer in terms of moles and an alkali in an amount of from 0.1 to 1.5 times the amount of the crosslinking agent in terms of moles are added continuously dropwise or added dividedly over a prescribed period of time, whereby flow rate characteristics of a resulting porous cellulose gel can be enhanced. According to the invention, the production efficiency of a polymer substance, such as a nucleic acid and a protein can be enhanced. 1. A porous cellulose gel comprising crosslinked cellulose particles having a solvent contained therein , upon filling the gel in a chromatography column having an inner diameter of 2.2 cm to a height of 17.5±2.5 cm , a linear velocity of water at 20° C. being from 2 ,400 to 4 ,500 cm/hour at a pressure of 0.4 MPa.2. A porous cellulose gel comprising crosslinked cellulose particles having a solvent contained therein , upon filling the gel in a chromatography column having an inner diameter of 2.2 cm to a height of 17.5±2.5 cm , a maximum linear velocity of water at 20° C. being from 2 ,400 to 5 ,500 cm/hour.3. The porous cellulose gel of claim 1 , wherein the crosslinked cellulose particles have a particle diameter of from 1 to 2 claim 1 ,000 μm.4. The porous cellulose gel of claim 1 , wherein the porous cellulose gel has an exclusion limit molecular weight with polyethylene glycol of from 1 claim 1 ,000 claim 1 ,000 to 5 claim 1 ,000 claim 1 ,000.5. The porous cellulose gel of claim 1 , wherein the crosslinked cellulose particles have a swelling degree of from 5 to 20 mL/g.6. The porous cellulose gel of claim 1 , wherein the crosslinked cellulose particles have a reswelling degree of from 80 to 100%.711-. (canceled)12. A filler for ...
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