10-12-2020 дата публикации
Номер: US20200385803A1
Автор:
Frank Bergmann,
Peter J. Crisalli,
Sara Kelley,
Omid Khakshoor,
Hannes Kuchelmeister,
Meng Taing,
BERGMANN FRANK,
CRISALLI PETER J,
KELLEY SARA,
KHAKSHOOR OMID,
KUCHELMEISTER HANNES,
TAING MENG,
Bergmann, Frank,
Crisalli, Peter J.,
Kelley, Sara,
Khakshoor, Omid,
Kuchelmeister, Hannes,
Taing, Meng
Принадлежит:
The disclosure relates to tagged nucleoside compounds comprising a nucleotide polyphosphate covalently attached to a tag, wherein the compound is a polymerase substrate and the polymer moiety is capable of entering a nanopore linked to the polymerase and thereby altering the flow of ions through the nanopore. The disclosure also provides methods for preparing the tagged nucleoside compounds and for their use as tags in nanopore-based nucleic acid detection and sequencing. 2. The compound of claim 1 , wherein the compound is a substrate for a polymerase linked to a nanopore.3. The compound of claim 1 , wherein x+y+z=30.4. The compound of claim 1 , wherein x=14-22.5. The compound of claim 1 , wherein Rand/or Rare independently selected from O and CH.6. The compound of claim 1 , wherein Band/or Bare independently selected from the group consisting of adenine claim 1 , cytosine claim 1 , guanine claim 1 , thymine claim 1 , uracil claim 1 , hypoxanthine claim 1 , N3CEdT claim 1 , N3MedT claim 1 , etheno-dA claim 1 , 5MedC claim 1 , 5MedC-PhEt claim 1 , and dCb.7. The compound of claim 1 , wherein Cap is a 3′-propanol group.8. The compound of claim 1 , wherein N is a unit of formula (2a).9. The compound of claim 1 , wherein x=14-22 claim 1 , y=6-10 claim 1 , z=3-6 claim 1 , x+y+z=30 claim 1 , N is a unit of formula (2a) claim 1 , and Cap is a 3′-propanol group.10. The compound of claim 9 , wherein Ris O and Bis a modified nucleobase.11. The compound of claim 10 , wherein the modified base is independently selected from N3CEdT claim 10 , N3MedT claim 10 , etheno-dA claim 10 , 5MedC claim 10 , 5MedC-PhEt claim 10 , and dCb.12. The compound of claim 9 , wherein Ris CHand B1 is thymine or hypoxanthine.14. The compound of claim 13 , wherein the linker comprises a chemical group selected from the group consisting of: ester claim 13 , ether claim 13 , thioether claim 13 , amine claim 13 , amide claim 13 , imide claim 13 , carbonate claim 13 , carbamate claim 13 , squarate claim ...
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