18-04-2013 дата публикации
Номер: US20130095554A1
The present invention relates to a modified Family 5 cellulase comprising a substitution of an amino acid at position 363 with a non-native alanine, serine or threonine, said position determined from alignment of the modified Family 5 cellulase with amino acids 71-397 of a Cel5A amino acid sequence as set forth in SEQ ID NO:1 and enzyme mixtures comprising same. Additionally provided is a genetic construct comprising a nucleic acid sequence encoding the modified Family 5 cellulase and a genetically modified microbe comprising the genetic construct. The invention also provides a process for producing the modified Family 5 cellulase. 1. A modified Family 5 cellulase comprising a substitution of an amino acid at position 363 with a non-native alanine , or serine or threonine , said position determined from alignment of the modified Family 5 cellulase amino acid sequence with amino acids 71 to 397 of SEQ ID NO:1 , wherein the modified Family 5 cellulase contains no more than 20 other amino acid substitutions relative to a corresponding wild-type Family 5 cellulase , and wherein the modified Family 5 cellulase is derived from a fungal parental Family 5 cellulase which does not naturally possess an alanine , serine , or threonine at position 363.2. The modified Family 5 cellulase of claim 1 , wherein said modified Family 5 cellulase exhibits an increase in specific activity of at least about 1.2 fold relative to a parental Family 5 cellulase or a corresponding wild-type Family 5 cellulase.3Trichoderma, Hypocrea, Penicillium, Botryotinia, Macrophomina, Aspergillus, Orpinomyces, Pestalotiopsis, MyceliopthoraChrysosporium.. The modified Family 5 cellulase of claim 1 , wherein said fungal parental Family 5 cellulase is a Family 5 cellulase from a species of claim 1 , or4. The modified Family 5 cellulase of claim 1 , wherein the substituted amino acid at position 363 is an alanine.5. An enzyme mixture comprising the modified Family 5 cellulase of .6. A process for bio-stoning ...
Подробнее
