25-11-2021 дата публикации
Номер: US20210360915A1
Принадлежит:
The present invention relates to cryopreserving compositions which are suitable for the cryopreservation of biological materials, e.g. cells and proteins. The cryopreserving compositions comprise specified concentrations of polyvinyl alcohol (PVA) and poly-ethylene glycol (PEG). The invention also relates to process for producing cryopreserved biological materials. 1. A composition comprising:(a) 0.1-40 mg/ml polyvinyl alcohol (PVA), and(b) 10-400 mg/ml poly-ethylene glycol (PEG).2. A composition as claimed in claim 1 , wherein:(a) the PVA concentration is 0.5 mg/mL to 10 mg/mL, preferably 0.7 mg/mL to 5 mg/mL, and more preferably about 1 mg/mL, wherein the composition additionally comprises biological material for cryopreservation; or(b) the PVA concentration is 1.0 mg/mL to 20 mg/mL, preferably 1.4 mg/mL to 10 mg/mL, and more preferably about 2 mg/mL.3. A composition as claimed in or claim 1 , wherein the PVA has a weight average molecular weight of 1-80 kDa or 3-50 kDa.4. A composition as claimed in claim 3 , wherein the PVA has a weight average molecular weight of 5-40 kDa or 6-14 kDa claim 3 , preferably 7-13 kDa claim 3 , more preferably 8-12 kDa or 9-11 kDa claim 3 , and most preferably about 10 kDa.5. A composition as claimed in any one of the preceding claims claim 3 , wherein:(a) the PEG concentration is 75-125 mg/mL, preferably about 100 mg/mL, wherein the composition additionally comprises biological material for cryopreservation; or(b) the PEG concentration is 150-250 mg/mL, preferably about 200 mg/mL.6. A composition as claimed in any one of the preceding claims claim 3 , wherein the PEG has a weight average molecular weight of 100 Da to 100 kDa.7. A composition as claimed in claim 6 , wherein the PEG has a weight average molecular weight of 200 Da-50 kDa claim 6 , 1-25 kDa claim 6 , 1-15 kDa claim 6 , 2-10 kDa claim 6 , or 3-5 kDa claim 6 , preferably about 4 kDa.8. A composition as claimed in claim 1 , wherein the composition comprises:(a) 0.5-5 mg/ ...
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