23-06-2023 дата публикации
Номер: CN116298248A
Принадлежит:
The invention discloses an aptamer sensor and method for detecting aflatoxin B1 based on an aggregation-induced emission effect, and belongs to the technical field of biological detection. The aptamer sensor comprises a single-stranded DNA aptamer specifically bound with aflatoxin B1, a small-molecular fluorescent dye capable of being bound with the single-stranded DNA aptamer and generating an aggregation-induced emission effect, and a carbon nanomaterial capable of performing fluorescence resonance energy transfer with the small-molecular fluorescent dye. When the aptamer sensor is used for detecting the aflatoxin B1, the aflatoxin B1 can be quantitatively detected according to the intensity change of a fluorescence signal. According to the method, high-sensitivity detection of aflatoxin B1 can be realized, the detection limit is low, the specificity is good, and an effective method is provided for detection of trace aflatoxin B1 and other harmful substances.
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