PROCESS CHAMBER WITH OPENINGS TO WOULD BRING IN A PIPETTE

ORIENTATIONSGERICHTETE CONSTRUCTION OF PLASMIDEN

PROCESSING CHAMBER WITH APERTURES FOR PIPETTE ACCESS

A device having a processing chamber, particularly for electroelution and/or dialysis of a substance carried in a sample with respect to an external liquid medium. The chamber is closed at one end and has an opening at the other longitudinal end sufficiently large to permit a sample, and in particular a gel contained sample, to be inserted thereinto. The chamber has a pair of portals laterally disposed with respect to the opening. The portals are covered with a typically tubular membrane, which is sealingly fixed onto an outside surface of the housing defining the chamber, typically by means of a tubular sleeve having portals that align with the portals of the chamber, via an annular sealing arrangement. The device provides high yield recovery, saves time, and allows for relatively easy handling specially regarding loading and unloading of small volume of samples to be dialyzed or inserting the slice of gel containing the macromolecule sample. The device may be used in an open manner by ...

ORIENTATIONSGERICHTETE KONSTRUKTION VON PLASMIDEN

BUFFER SYSTEM FOR A LONG-LIVED FINISHED POURING ELECTROPHORESIS GEL

GEL FOR ELECTROPHORESIS

Processing chamber

Regulation of gene expression through manipulation of mrna splicing and its uses

ORIENTATIONSGERICHTETE KONSTRUKTION VON PLASMIDEN

Processing chamber

PROZESSKAMMER MIT ÖFFNUNGEN ZUM EINFÜHREN EINER PIPETTE

Gel trap for electrophoresis

Gel for electrophoresis

The present invention is directed to a solidified hybrid gel for use in an electrophoresis process, having a solidified first gel portion juxtaposed with a solidified second gel portion. The first gel portion is capable of accommodating therein one or more samples for electrophoresis after said first gel portion is in solidified form, and the second gel portion is adapted for enabling an electrophoresis process to be applied to such a sample that may be accommodated in said first gel portion. Thus, the hybrid gel may be provided in a precast form to users, ready for use. The invention is also directed to methods for providing such a gel, apparatuses for accommodating such a gel, and methods for carrying out electrophoresis on a sample comprised in such a gel.

Prozesskammer mit Öffnungen zum Einführen einer Pipette

GEL FÜR ELEKTROPHORESE

GEL FÜR ELEKTROPHORESE

BUFFER SYSTEM FOR A LONG-LASTING PRECAST ELECTROPHORESIS GEL

Provided is a precast polyacrylamide gel for use in gel electrophoresis, having a shelf-life of at least 12 months. The gel comprises a sulfonated tertiary or secondary amine having a pKa of 6.6-8.0, ampholytes exhibiting pI between 5.4 to 6.4, Tris, and hydrochloric acid to adjust the pH to between 5.5 and 7.5.

DISPOSABLE INDEPENDENT 3-D STRUCTURE DEPENED SEQUENTIAL CAPILLARY LATERAL FLOW DEVICE FOR ANALYTE DETERMINATION

The present invention provides a disposable 3-D structured depended sequential lateral flow capillary device comprising: a housing; a 3-D structured capillary flow matrix; and (iii) an absorption portion, and use thereof in a method for determining the presence of an analyte in a sample.

Brucella phage polynucleotides and uses thereof

An isolated polynucleotide is disclosed which comprises a nucleic acid sequence of a Brucella phage, the nucleic acid sequence being specific to the Brucella phage and comprising a sequence selected from the group consisting of SEQ ID NOs: 387-393. An exemplary polynucleotide sequence is one which comprises at least 100 consecutive nucleotides of a nucleic acid sequence as set forth in SEQ ID NO: 396. Uses of such sequences are further disclosed.

Processing chamber

A device having a processing chamber, for electroelution and/or dialysis of a substance carried in a sample with respect to an external liquid medium. The chamber has a closed end and an opening at the other end, sufficiently large to permit a sample, particularly a gel contained sample, to be inserted thereto. The chamber has two portals, each laterally disposed with respect to the opening. A tubular membrane covers the portals, and is sealingly fixed onto an outside surface of the housing. The device provides high yield recovery, saves time and allows for easy handling, especially regarding loading an unloading of small volume samples for dialysis, or inserting the gel slice containing the macromolecule sample. The device may be partially immersed in the liquid medium, with the opening above the liquid surface. Optionally, the device may be hermetically sealed via a cap, and fully immersed in the liquid medium.

Orientation-directed construction of plasmids

A method for orientation-directed construction of a construct including at least two nucleic acid segments of interest. Products are provided having phosphorylated blunt ends derived from the nucleic acid segments of interest. Separate ligation reactions are performed wherein in each reaction the phosphorylated blunt-ended products of two different segments obtained in the previous step are ligated to create a combined ligated sequence. PCR amplification reaction is performed using the combined ligated sequence obtained in the previous step as a template and specific enrichment primers direct the PCR amplification towards the desired orientation. Each combined ligated sequence is amplified in a separate reaction creating a combined product having phosphorylated blunt ends. The combined product is isolated and purified to have the enriched desired orientation. Self-ligation of the isolated purified combined product is performed to create a circular double-stranded DNA construct containing the desired segments properly aligned and operably linked.

LONG-LASTING PRECAST ELECTROPHORESIS GEL

Disclosed is a precast polyacrylamide gel for use in gel electrophoresis, comprising polyacrylamide and an aqueous Tris 0.04 M to 0.15 M solution, at least one first ampholyte exhibiting an isoelectric point (pI) of from 5.4 to 6.4 at a total concentration of from 0.01 M to 0.4 M; and at least one second ampholyte exhibiting an isoelectric point (pI) of from 2.5 to 3.5 to adjust the pH to between 5.5 and 7.5 at the temperature of 25° C. The precast gels can be used for electrophoresis of oligopeptides, polypeptides, oligonucleotides, and polynucleotides under denaturating or nondenaturing conditions, and exhibit long shelf-life.

BRUCELLA PHAGE POLYNUCLEOTIDES AND USES THEREOF

An isolated polynucleotide is disclosed which comprises a nucleic acid sequence of a Brucella phage, the nucleic acid sequence being specific to the Brucella phage and comprising a sequence selected from the group consisting of SEQ ID NOs: 387-393. An exemplary polyncucleotide sequence is one which comprises at least 100 consecutive nucleotides of a nucleic acid sequence as set forth in SEQ ID NO: 396. Uses of such sequences are further disclosed.

Regulation of gene expression through manipulation of mRNA splicing and its uses

A cis-acting nucleotide sequence which is capable of rendering the removal of introns from a precursor transcript encoded by a gene, which gene harbors at least one such cis-acting nucleotide sequence, occurring during the production of mRNA of a gene, dependent upon activation of a trans-acting factor. The trans-acting factor is an RNA-activated protein kinase which is capable of phosphorylating the alpha-subunit of eukaryotic initiation factor 2. The trans-acting factor is preferably, the RNA-activated protein kinase (PKR). The cis-acting nucleotide sequence can be derived from the 3' untranslated region of the human tumor necrosis factor alpha gene (TNF-alpha3'-UTR) and may comprise the nucleotide sequence as denoted by SEQ ID NO:1 or biologically functional fragments, derivatives, mutants and homologues thereof.

Double chamber tank for horizontal gel electrophoresis

A tank apparatus (10) for horizontal electrophoresis that comprises a base member (12) having at least first and second independent receptacles (16, 18) for accommodating buffer solution. Each of the receptacles comprises an inlet (28, 28) for allowing communication with the external environment. A cover member (14) covers at least the first and second receptacles, thereby forming first and second buffer chambers. The cover member comprising suitable openings (40, 42) for allowing a portion of an electrophoresis gel cassette to be inserted therethrough, such that the electrophoresis gel is in communication with the contents of each of the first and second receptacles. Each of a pair of electrodes (33, 33) is situated in one of each of the first and second receptacles, wherein the electrodes are connectable to an electrical power supply.

Brucella phage polynucleotides and uses thereof

An isolated polynucleotide is disclosed which comprises a nucleic acid sequence of a Brucella phage, the nucleic acid sequence being specific to the Brucella phage and comprising a sequence selected from the group consisting of SEQ ID NOs: 387-393. An exemplary polynucleotide sequence is one which comprises at least 100 consecutive nucleotides of a nucleic acid sequence as set forth in SEQ ID NO: 396. Uses of such sequences are further disclosed.

Gel trap for electrophoresis

An apparatus for electrophoresis having a first gel matrix, adapted for performing an electrophoretic process therein, in communication with a second gel matrix, both being accommodated within a suitable housing. The housing has a first opening adapted to permit ionic communication between the first gel matrix and an external ionic buffer solution, and a second opening adapted to permit ionic communication between the second gel matrix and an external ionic buffer solution. The second gel has at least one suitable absorption material capable of retaining therein at least one target substance capable of migrating thereto from the first gel matrix when an electrophoretic process is performed in the first matrix.

BRUCELLA PHAGE POLYNUCLEOTIDES AND USES THEREOF

An isolated polynucleotide is disclosed which comprises a nucleic acid sequence of a Brucella phage, the nucleic acid sequence being specific to the Brucella phage and comprising a sequence selected from the group consisting of SEQ ID NOs: 387-393. An exemplary polynucleotide sequence is one which comprises at least 100 consecutive nucleotides of a nucleic acid sequence as set forth in SEQ ID NO: 396. Uses of such sequences are further disclosed.

BRUCELLA PHAGE POLYNUCLEOTIDES AND USES THEREOF

An isolated polynucleotide is disclosed which comprises a nucleic acid sequence of a phage, the nucleic acid sequence being specific to the phage and comprising a sequence selected from the group consisting of SEQ ID NOs: 387-393. An exemplary polynucleotide sequence is one which comprises at least 100 consecutive nucleotides of a nucleic acid sequence as set forth in SEQ ID NO: 396. Uses of such sequences are further disclosed. 1. An isolated polynucleotide comprising:(a) at least 100 consecutive nucleotides of the nucleic acid sequences selected from the group consisting of 387-393;(b) a heterologous nucleic acid sequence; and(c) a heterologous sequence which directs expression of said heterologous nucleic acid sequence, wherein said at least 100 consecutive nucleotides is positioned downstream to said (b) heterologous nucleic acid sequence.2. The isolated polynucleotide of further comprising at least one nucleic acid sequence being selected from the group consisting of SEQ ID NO: 394 and 395 in a forward or reverse orientation.3. The isolated polynucleotide of claim 1 , wherein said heterologous nucleic acid sequence encodes a detectable moiety.4Brucella.. The isolated polynucleotide of claim 1 , wherein said heterologous nucleic acid sequence encodes a polypeptide which is lethal to5. A method of down-regulating expression of a gene of interest in a bacteria claim 1 , the method comprising transforming bacteria with a nucleic acid construct which comprises the isolated polynucleotide of claim 1 , thereby down-regulating expression of the gene of interest.6Brucella. The method of claim 4 , wherein said bacteria comprises bacteria.7BrucellaB. SuisB. melitensis.. The method of claim 6 , wherein a strain of said bacteria comprises or8. The method of claim 5 , wherein the gene is endogenous to the bacteria.9. The method of claim 5 , wherein the gene is endogenous to a phage of the bacteria.10. The method of claim 5 , wherein said regulatory sequence is flanked by a ...

Processing chamber

A device having a processing chamber, particularly for electroelution and/or dialysis of a substance carried in a sample with respect to an external liquid medium. The chamber is closed at one end and has an opening at the other longitudinal end sufficiently large to permit a sample, and in particular a gel contained sample, to be inserted thereinto. The chamber has a pair of portals laterally disposed with respect to the opening. The portals are covered with a typically tubular membrane, which is sealingly fixed onto an outside surface of the housing defining the chamber, typically by means of a tubular sleeve having portals that align with the portals of the chamber, via an annular sealing arrangement. The device provides high yield recovery, saves time, and allows for relatively easy handling specially regarding loading and unloading of small volume of samples to be dialyzed or inserting the slice of gel containing the macromolecule sample. The device may be used in an open manner by partially immersing the same in the liquid medium with the opening above the liquid surface. Optionally, the device may be closed and hermetically sealed if so desired by means of a cap, and thus immersed in a liquid medium.

Processing chamber

A device having a processing chamber, particularly for electroelution and/or dialysis of a substance carried in a sample with respect to an external liqu id medium. The chamber is closed at one end and has an opening at the other longitudinal end sufficiently large to permit a sample, and in particular a gel contained sample, to be inserted thereinto. The chamber has a pair of portals laterally disposed with respect to the opening. The portals are covered with a typically tubular membrane, which is sealingly fixed onto an outside surface of the housing defining the chamber, typically by means of a tubular sleeve having portals that align with the portals of the chamber, vi a an annular sealing arrangement. The device provides high yield recovery, sav es time, and allows for relatively easy handling specially regarding loading an d unloading of small volume of samples to be dialyzed or inserting the slice o f gel containing the macromolecule sample. The device may be used in an open manner by partially immersing the same in the liquid medium with the opening above the liquid surface. Optionally, the device may be closed and hermetically sealed if so desired by means of a cap, and thus immersed in a liquid medium.

Gel for electrophoresis

The present invention is directed to a solidified hybrid gel for use in an electrophoresis process, having a solidified first gel portion juxtaposed with a solidified second gel portion. The first gel portion is capable of accommodating therein one or more samples for electrophoresis after said first gel portion is in solidified form, and the second gel portion is adapted for enabling an electrophoresis process to be applied to such a sample that may be accommodated in said first gel portion. Thus, the hybrid gel may be provided in a precast form to users, ready for use. The invention is also directed to methods for providing such a gel, apparatuses for accommodating such a gel, and methods for carrying out electrophoresis on a sample comprised in such a gel.

Gel for electrophoresis

The present invention is directed to a solidified hybrid gel for use in an electrophoresis process, having a solidified first gel portion juxtaposed with a solidified second gel portion. The first gel portion is capable of accommodating therein one or more samples for electrophoresis after said first gel portion is in solidified form, and the second gel portion is adapted for enabling an electrophoresis process to be applied to such a sample that may be accommodated in said first gel portion. Thus, the hybrid gel may be provided in a precast form to users, ready for use. The invention is also directed to methods for providing such a gel, apparatuses for accommodating such a gel, and methods for carrying out electrophoresis on a sample comprised in such a gel.

Gel for electrophoresis

The present invention is directed to a solidified hybrid gel for use in an electrophoresis process, having a solidified first gel portion juxtaposed with a solidified second gel portion. The first gel portion is capable of accommodating therein one or more samples for electrophoresis after said first gel portion is in solidified form, and the second gel portion is adapted for enabling an electrophoresis process to be applied to such a sample that may be accommodated in said first gel portion. Thus, the hybrid gel may be provided in a precast form to users, ready for use. The invention is also directed to methods for providing such a gel, apparatuses for accommodating such a gel, and methods for carrying out electrophoresis on a sample comprised in such a gel.

Gel trap for electrophoresis

An apparatus for electrophoresis having a first gel matrix, adapted for performing an electrophoretic process therein, in communication with a second gel matrix, both being accommodated within a suitable housing. The housing has a first opening adapted to permit ionic communication between the first gel matrix and an external ionic buffer solution, and a second opening adapted to permit ionic communication between the second gel matrix and an external ionic buffer solution. The second gel has at least one suitable absorption material capable of retaining therein at least one target substance capable of migrating thereto from the first gel matrix when an electrophoretic process is performed in the first matrix.

Gel für elektrophorese

Puffersystem für ein langlebiges fertig gegossenes elektrophoresegel

Long-lasting precast electrophoresis gel

Disclosed is a precast polyacrylamide gel for use in gel electrophoresis, comprising polyacrylamide and an aqueous Tris 0.04 M to 0.15 M solution, at least one first ampholyte exhibiting an isoelectric point (pI) of from 5.4 to 6.4 at a total concentration of from 0.01 M to 0.4 M; and at least one second ampholyte exhibiting an isoelectric point (pI) of from 2.5 to 3.5 to adjust the pH to between 5.5 and 7.5 at the temperature of 25°C. The precast gels can be used for electrophoresis of oligopeptides, polypeptides, oligonucleotides, and polynucleotides under denaturating or nondenaturing conditions, and exhibit long shelf-life.

Prozesskammer mit öffnungen zum einführen einer pipette

Orientationsgerichtete konstruktion von plasmiden

Regulation of gene expression through manipulation of mRNA splicing and its uses

A cis-acting nucleotide sequence which is capable of rendering the removal of introit/s from a precursor transcript encoded by a gene, which gene harbors at least one such cis-acting nucleotide sequence, occurring during the production of mRNA of said gene, dependent upon activation of a trans-acting factor. The trans-acting factor is an RNA-activated protein kinase which is capable of phosphorylated the α-subunit of eucaryotic initiation factor 2, or the RNA-activated protein kinase (PAR). The cis-acting nucleotide sequence can be derived from the 3′ untranslated region of the human tumor necrosis factor α gene (TNF-α 3′-UTR). The cis-acting nucleotide sequence may comprise the nucleotide sequence as denoted by SEQ ID NO: 1; or biologically functional fragments, derivatives, mutants and homologues of the nucleotide sequence as denoted by SEQ ID NO: 1; or a nucleotide sequence whose complementary nucleotide sequence hybridizes, under conditions which allow for such hybridization to occur, with the nucleotide sequences as denoted by SEQ ID NO: 1 or biologically functional fragments, derivatives, mutants and homologues of the nucleotide sequence as denoted by SEQ ID NO: 1.

REGULATION OF GENE EXPRESSION THROUGH MANIPULATION OF mRNA SPLICING AND ITS USES

A cis-acting nucleotide sequence which is capable of rendering the removal of intron/s from a precursor transcript encoded by a gene, which gene harbors at least one such cis-acting nucleotide sequence, occurring during the production of mRNA of said gene, dependent upon activation of a trans-acting factor. The trans-acting factor is an RNA-activated protein kinase which is capable of phosphorylating the α-subunit of eukaryotic initiation factor 2, or the RNA-activated protein kinase (PKR). The cis-acting nucleotide sequence can be derived from the 3' untranslated region of the human tumor necrosis factor α gene (TNF-α 3'-UTR). The cis-acting nucleotide sequence may comprise the nucleotide sequence substantially as denoted by SEQ ID NO:1; or biologically functional fragments, derivatives, mutants and homologues of the nucleotide sequence substantially as denoted by SEQ ID NO:1; or a nucleotide sequence whose complementary nucleotide sequence hybridizes, under conditions which allow for such hybridization to occur, with the nucleotide sequences substantially as denoted by SEQ ID NO:1 or biologically functional fragments, derivatives, mutants and homologues of the nucleotide sequence substantially as denoted by SEQ ID NO:1.