21-05-2015 дата публикации
Номер: US20150141714A1
Принадлежит:
The disclosed invention provides methods and compositions for increasing terpenoid production, such as sesquiterpenoids, such as farnesene, in plant cells. 1. A plant cell having increased production of at least one terpenoid native to a plant , the method comprising expressing in a plant cell a heterologous nucleic acid encoding for (a) HMG-CoA reductase , (b) 1-deoxy-D-xylulose-5-phosphate synthase , (c) farnesyl pyrophosphate synthase , and (d) β-farnesene synthase , wherein production of the at least one terpenoid is significantly increased when compared to a wild-type plant cell not encoding the heterologous nucleic acids.2. The method of claim 1 , wherein{'i': Arabidopsis, Oryza, Saccharomyces', 'Hevea, 'a. the HMG-CoA reductase is an , or HMG-CoA reductase;'}{'i': Arabidopsis, Oryza, Saccharomyces', 'Zea, 'b. the 1-deoxy-D-xyululose-5-phophate is an , or 1-deoxy-D-xyululose;'}{'i': Arabidopsis, Oryza', 'Solanum, 'c. the farnesyl pyrophosphate synthase is an , or farnesyl pyrophosphate; or'}{'i': Arabidopsis, Oryza', 'Artemisia, 'd. the β-farnesene synthase is an , or β-farnesene synthase.'}3. The method of claim 2 , wherein{'i': Arabidopsis thaliana, Oryza sativa, Saccharomyces cerevisiae', 'Hevea, 'a. the HMG-CoA reductase is an , or HMG-CoA reductase;'}{'i': Arabidopsis thaliana, Oryza sativa, Saccharomyces cerevisiae', 'Zea mays, 'b. the 1-deoxy-D-xyululose-5-phophate is an , or 1-deoxy-D-xyululose;'}{'i': Arabidopsis thaliana, Oryza sativa', 'Solanum lycopersicon, 'c. the farnesyl pyrophosphate synthase is an , or farnesyl pyrophosphate;'}{'i': Arabidopsis thaliana, Oryza sativa', 'Artemisia annua β, 'd. the β-farnesene synthase is an , or -farnesene synthase.'}4. The method of claim 3 , wherein at least one nucleic acid is codon-optimized for expression in a plant.5. The method of claim 3 , whereina. the HMG-CoA reductase is encoded by a polynucleotide having at least 70% sequence identity to a nucleic acid sequence selected from the group consisting of ...
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