METHOD AND APPARATUS FOR FLUID DISPERSION

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques. 1. A device comprising:a microfluidic interconnected region comprising an upstream portion, a downstream portion and a dimensionally-restricted section defining the downstream portion of the microfluidic interconnected region, the microfluidic interconnected region being connected at its upstream portion to two or more microfluidic channels.2. The device of claim 1 , wherein the dimensionally-restricted section comprises a non-valved orifice.3. The device of claim 1 , wherein the dimensionally-restricted section is formed by at least an extension of a wall defining the interconnected region.4. The device of claim 1 , wherein the microfluidic interconnected region and the two or more microfluidic channels are part of a single integral unit.5. The device of claim 1 , wherein the microfluidic interconnected region claim 1 , the upstream portion claim 1 , and the downstream portion are each contained within a microfluidic device.6. The device of claim 1 , wherein the microfluidic interconnected region has a maximum cross-sectional diameter of less than 50 microns.7. The device of claim 1 , wherein the downstream portion has a largest dimension perpendicular to fluid flow of less than about 1 mm.8. The device of claim 1 , wherein the device comprises two microfluidic inlet channels each containing a continuous fluid and one microfluidic inlet channel containing a subject fluid.9. The device of claim 8 , wherein the subject fluid forms discontinuous sections at the interconnected region surrounded by the continuous fluid claim 8 , at least some of the discontinuous sections having a maximum dimension of less than 100 microns.10. The device of claim 8 , wherein the continuous fluid completely circumferentially surrounds the subject ...

System and method for automated generation and handling of liquid mixtures

The invention relates to a system (1) for supplying a microfluidic subsystem with liquids, comprising a first valve (14, 29, 46) and a first fluidic duct (10, 25, 28), for connecting said first valve (14, 29, 46) with said microfluidic subsystem and supplying a first liquid, and a second fluidic duct (11), for connecting with said microfluidic subsystem and supplying a second liquid characterized in that said first valve (14, 29, 46) is suitable for closing with time resolution not worse than 100 msec, and parameters of said first fluidic duct (10, 15, 28) are chosen such that the value of X1[Pa−1], defined as: X1[Pa−1]=(0.5×10−9+1/E1)(αR1L12/A1) is lower 104 Pa−1, where E1 is the Young modulus of the material, of which said first fluidic duct (10, 25, 28) is made, L1 is the length of the said first fluidic duct (10, 25, 28), A1 is the surface area of the lumen of the said first fluidic duct (10, 25, 28) and αR1 is a constant characterizing the geometry of the said first fluidic duct (10 ...

Method to modify surfaces of microchannels fabricated in a polycarbonate object and a polycarbonate object containing a microchannel modified with the method

Method for producing capsules with hydrophilic core and polymer shell

Capsules with hydrophilic core and polymer shell and method for producing thereof

System and method of obtaining entrained cylindrical fluid flow

A method and system for entraining fluids is provided. The method and system may be used to create filaments. As one example, a filament may be produced by entraining a first fluid within a second fluid by flowing a third fluid, the flowing third fluid at least partly constraining the second fluid in at least one dimension. As another example, a filament may be produced by entraining a first fluid within a second fluid based on a model of a dynamic response of the first and second fluids as functions of densities of the first and second fluids, viscosities of the first and second fluids, Reynolds numbers of the first and second fluids, and Weber numbers of the first and second fluids.

METHOD FOR SPLITTING DROPLETS ON DEMAND IN MICROFLUIDIC JUNCTION

The invention relates to a method for splitting droplets on demand in a microfluidic junction, comprising the supply channel, the first drain channel and the second drain channel, the method comprises the following steps: 114-. (canceled)15. A method for splitting droplets on demand in a microfluidic junction , comprising a supply channel , a first drain channel and a second drain channel , the method comprising the steps of:delivering a droplet to said microfluidic junction through the supply channel by means of a flow of continuous liquid through said supply channel and said first drain channel;stopping the flow in said first drain channel and opening the flow in said second drain channel until a fraction of the droplet is present in the second drain channel;resuming the flow in the first drain channel and closing the flow in the second drain channel, at least until the fraction of said droplet being present in the first drain channel separates from the rest of the droplet.16. The method according to claim 15 , wherein the flows are controlled automatically claim 15 , with a sensor claim 15 , preferably a camera claim 15 , located in the vicinity of said microfluidic junction and connected directly or indirectly to three valves controlling the flows in said supply channel claim 15 , said first drain channel and said second drain channel claim 15 , respectively.17. The method according to claim 15 , wherein said microfluidic junction is a T-junction where the supply channel claim 15 , the first drain channel claim 15 , and the second drain channel form with each other angles of 180° claim 15 , 90° claim 15 , and 90° claim 15 , respectively.18. The method according to claim 15 , wherein said microfluidic junction is a Y-junction where the supply channel claim 15 , the first drain channel claim 15 , and the second drain channel form with each other angles of 150° claim 15 , 60° and 150° claim 15 , respectively.19. The method according to claim 15 , wherein said ...

Fluid waveguide and uses thereof

The invention relates to methods and apparatuses for guiding and emitting electromagnetic radiation from a fluid waveguide. Various methods for changing optical properties (e.g., refractive index, absorption, and fluorescence) and/or physical properties (e.g., magnetic susceptibility, electrical conductivity, and temperature) of either the waveguide core or the cladding, or both, are provided herein. In one embodiment, electromagnetic radiation is guided and/or emitted at multiple distinct wavelengths, including emission in the form of an essentially continuous band, in some cases covering at least 150 nanometers. In another embodiment, methods for splitting a waveguide core and/or the joining of at least two waveguide cores in a waveguide are provided. In yet another embodiment, the invention includes the use of thermal gradients to generate a waveguide and/or to change the properties of waveguides. Embodiments of the waveguides may be used for optical detection or spectroscopic analysis ...

Method and apparatus for fluid dispersion

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.

METHOD FOR PERFORMING QUANTITATION ASSAYS

The present invention relates to a method for determining an estimate of a concentration of particles E(C), wherein a sample of predetermined volume is divided into a number (N) of compartments, the (N) compartments comprise or consist of different sample volumes (v) and/or different dilution factors (d) of the sample, at least part of the particles that are present in any of the (N) compartments provide a measurable signal and the estimated concentration of particles E(C) is a function of measured signals, as well as an apparatus for use in the inventive method, uses of the inventive method, a sample holder and a kit for use in the inventive method. 2. The method of claim 1 , wherein at least 25% of compartments differ from each other by different sample volumes and/or sample dilutions.3. The method of claim 1 , wherein at least 50% of compartments differ from each other by different sample volumes and/or sample dilutions.4. The method of claim 1 , wherein at least 75% of compartments differ from each other by different sample volumes and/or sample dilutions.5. The method of claim 1 , wherein 100% of compartments differ from each other by different sample volumes and/or sample dilutions.6. The method of claim 1 , wherein the particles are amplified prior to measuring the signal from the particles in the compartments by a polymerase chain reaction (PCR) claim 1 , loop-mediated isothermal amplification (LAMP) claim 1 , rolling circle amplification (RCA) claim 1 , cascade rolling circle amplification (Cascade RCA) claim 1 , helicase-dependent amplification claim 1 , nucleic acid sequence based amplification (NASBA) claim 1 , nicking enzyme amplification reaction (NEAR) claim 1 , single-molecule enzyme-linked immunoabsorbent assay (ELISA) claim 1 , or a combination thereof.7. The method of claim 1 , wherein the particles are selected from the group consisting of: a virus claim 1 , a bacterium claim 1 , a nucleic acid claim 1 , a deoxyribonucleic acid (DNA) claim 1 , or ...

Device for simultaneous and uniform thermal cycling of samples and uses thereof

The invention relates to thermal cycling device comprising: a sample location; a first heating means, wherein advantageously said first heating means is a contact heating means; a second heating means, wherein said second heating means is configured to bring said sample to a second temperature by directing electromagnetic radiation to the first light pipe section end and the light pipe section is configured to direct said electromagnetic radiation through its second end to the sample location, its uses and methods based thereon.

SYSTEM AND METHOD FOR AUTOMATED GENERATION AND HANDLING OF LIQUID MIXTURES

The invention relates to a system ( 1 ) for supplying a microfluidic subsystem with liquids, comprising a first valve ( 14, 29, 46 ) and a first fluidic duct ( 10, 25, 28 ), for connecting said first valve ( 14, 29, 46 ) with said microfluidic subsystem and supplying a first liquid, and a second fluidic duct ( 11 ), for connecting with said microfluidic subsystem and supplying a second liquid characterized in that said first valve ( 14, 29, 46 ) is suitable for closing with time resolution not worse than 100 msec, and parameters of said first fluidic duct ( 10, 15, 28 ) are chosen such that the value of X 1 [Pa −1 ], defined as: X 1 [Pa −1 ]=(0.5×10 −9+1/ E 1 )(α R1 L 1 2 /A 1 ) is lower than 10 4 Pa −1 , where E 1 is the Young modulus of the material, of which said first fluidic duct ( 10, 25, 28 ) is made, L 1 is the length of the said first fluidic duct ( 10, 25, 28 ), A 1 is the surface area of the lumen of the said first fluidic duct ( 10, 25, 28 ) and α R1 is a constant characterizing the geometry of the said first fluidic duct ( 10, 25, 28 ) in an equation for the hydraulic resistance R 1 of the said first fluidic duct: R 1 =α R1 ( L 1 μ/A 1 2 ) with μ denoting the dynamic viscosity coefficient of the fluid filling the said first fluidic duct ( 10, 25, 28 ) in the measurement of R 1 . The invention relates also to a method for producing microdroplets on demand in such a system.

Method for performing quantitation assays

The present invention relates to a method for determining an estimate of a concentration of analyte particles E(C) as well as an apparatus for use in the inventive method, uses of the inventive method or the inventive apparatus, a sample holder and a kit for use in the inventive method.

Method and apparatus for fluid dispersion

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.

Microfluidic mixer

The present invention relates generally to microfluidic systems and, more specifically, to apparatuses and methods associated with mixing in microfluidic systems. In some embodiments, a mixer is constructed and arranged to mix at least a portion of a first and a second fluid component. The mixer may include a channel having an inlet that separates into at least two branches, the branches then recombining into a single outlet. In some cases, plugs of fluid (e.g., a gas) are flowed into the branches, which causes changes in resistance, and thus the amount of fluid flow, in each of the branches. The motion of the plugs through the network of branched channels can create unsteady mixing flows. For instance, for two fluid components, e.g., two streams of fluid flowing laminarly in the channel, these changes in resistance can cause the crossing of laminar streamlines of the fluid, which can lead to exponential stretching and folding of the interface between the two unmixed streams.

Microfluidic chip

A microfluidic chip for conducting microbiological assays, comprises a substrate in which incubation segments, a sample reservoir and microfluidic channels connecting said sample reservoir with said incubation segments are arranged. Said microfluidic chip further comprise a non-aqueous liquid reservoir for containing non-aqueous liquid wherein said reservoir is connectable via a releasable airtight and liquid-tight valve with said microfluidic channels connecting said sample reservoir with said incubation segments each incubation segment comprises an incubation well (113) connected by a gas-exchange channel (115) to an unvented gas cavity (111).

Microfluidic chip and valve, production process and uses

The present invention relates to a microfluidic chip and valve, production process and uses thereof according to the independent claims.

Systems and methods of forming particles

The present invention generally relates to systems and methods of forming particles and, in certain aspects, to systems and methods of forming particles that are substantially monodisperse. Microfluidic systems and techniques for forming such particles are provided, for instance, particles may be formed using gellation, solidification, and/or chemical reactions such as cross-linking, polymerization, and/or interfacial polymerization reactions. In one aspect, the present invention is directed to a plurality of particles having an average dimension of less than about 500 micrometers and a distribution of dimensions such that no more than about 5% of the particles have a dimension greater than about 10% of the average dimension, which can be made via microfluidic systems. In one set of embodiments, at least some of the particles may comprise a metal, and in certain embodiments, at least some of the particles may comprise a magnetizable material. In another set of embodiments, at least some of the particles may be porous. In some embodiments, the invention includes non-spherical particles. Non-spherical particles may be formed, for example, by urging a fluidic droplet into a channel having a smallest dimension that is smaller than the diameter of a perfect mathematical sphere having a volume of the droplet, and solidifying the droplet, and/or by exposing at least a portion of a plurality of particles to an agent able to remove at least a portion of the particles.

Method and apparatus for fluid dispersion

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.

METHOD FOR DETERMINING BIOCHEMICAL PARAMETERS OF A BODY FLUID

The invention relates to a method for determining biochemical parameters of a body fluid, wherein a sample of said body fluid in the form of a droplet is transported through a channel of a microfluidic system using a carrier liquid, mixed with a reagent thus initiating a chemical reaction between the sample and the reagent, and the result of the chemical reaction is measured, preferably with a spectrophotometer, whereby the said biochemical parameters of the body fluid are determined, characterised in that the material used for fabrication of the microfluidic system and the said carrier liquid is pair of Teflon and Fluorinert HFE-7100. 1. A method for determining biochemical parameters of a body fluid , wherein a sample of said body fluid in the form of a droplet is transported through a channel of a microfluidic system using a carrier liquid , mixed with a reagent thus initiating a chemical reaction between the sample and the reagent , and the result of the chemical reaction is measured , preferably with a spectrophotometer , whereby the said biochemical parameters of the body fluid are determined , characterised in that the material used for fabrication of the microfluidic system and the said carrier liquid is pair of Teflon and Fluorinert HFE-710.2. Method according to claim 1 , characterised in that the said reagent is selected from the group comprising: acp (acid phosphatase) claim 1 , alat (alanine aminotransferase) claim 1 , albumin claim 1 , alp (alkaline phosphatase) claim 1 , alpha-fetoprotein claim 1 , alpha-1-microglobulin claim 1 , amylase claim 1 , asat (aspartate transaminase) claim 1 , aso (anti-streptolysin O) claim 1 , bil direct (direct bilirubin) claim 1 , bil total (total bilirubin) claim 1 , calcium claim 1 , ceruloplasmin claim 1 , cholesterol claim 1 , cholinesterase claim 1 , ck (creatine kinase) claim 1 , ck MB (creatine kinase MB) claim 1 , complement C3 claim 1 , complement C4 claim 1 , crp (C-reactive protein) claim 1 , cystatin C claim ...

METHOD AND APPARATUS FOR FLUID DISPERSION

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.

Method and apparatus for fluid dispersion

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.

MICROFLUIDIC CHIP

A microfluidic chip for conducting microbiological assays, comprises a substrate in which incubation segments, a sample reservoir and microfluidic channels connecting said sample reservoir with said incubation segments are arranged. Said microfluidic chip further comprise a non-aqueous liquid reservoir for containing non-aqueous liquid wherein said reservoir is connectable via a releasable airtight and liquid-tight valve with said microfluidic channels connecting said sample reservoir with said incubation segments each incubation segment comprises an incubation well () connected by a gas-exchange channel () to an unvented gas cavity (). 115-. (canceled)16. A microfluidic chip for conducting microbiological assays , comprising a substrate made of impermeable material with a first major face and a , preferably parallel , second major face , wherein the surface of the first major face is covered by a first layer of impermeable , substantially transparent material and the surface of the second major face is covered by a second layer of impermeable , substantially transparent material , and within the substrate are arranged: a plurality of incubation segments each with an inlet channel ,a sample reservoir with an inlet opening and an outlet opening , and microfluidic channels connecting the outlet opening of said sample reservoir with each inlet channel to said incubation segments,wherein said microfluidic chip further comprises a non-aqueous fluid reservoir for containing non-aqueous liquid,wherein said non-aqueous fluid reservoir has an outlet opening which is connectable via a releasable airtight and liquid-tight valve with said microfluidic channels,wherein each incubation segment comprises an incubation well connected by a gas-exchange channel to an unvented gas cavity and the incubation segments are arranged in a fractal manner in which the respective microchannels connecting each of the incubation segments to the sample reservoir are substantially equally long and ...

MIKROFLUIDISCHER CHIP WITH AN UNVENTED GAS CAVITY IN A MICROFLUIDIC CHIP

An incubation segment in a microfluidic chip for microbiological assays, wherein said incubation segment is formed in a substrate with an upper major face and a lower major face, said segment includes an incubation well, an inlet channel through which a sample may be inputted into said incubation well, and a gas cavity connected to said incubation well by a microfluidic communication channel, wherein said gas cavity is unvented and the base of the gas cavity is connected to the base of the incubation well by the communication channel. 112-. (canceled)13. An incubation segment in a microfluidic chip for microbiological assays , wherein said incubation segment is formed in a substrate with an upper major face and a lower major face , said segment comprises an incubation well , an inlet channel through which a sample may be inputted into said incubation well , and a gas cavity connected to said incubation well by a microfluidic communication channel , wherein said gas cavity is unvented and that a base of the gas cavity is connected to a base of the incubation well by the communication channel.14. The incubation segment of claim 13 , wherein the volume of the incubation well Vis greater or equal to 0.5 μl and less than or equal to 5 μl claim 13 , the volume of the gas cavity Vg is greater or equal to 0.5 μl and less than or equal to 1.5 μl.15. The incubation segment of claim 14 , wherein the volume of the incubation well Vis greater or equal to 1 μl and less than or equal to 2.5 μl claim 14 , the volume of the gas cavity Vg is greater or equal to 0.7 μl and less than or equal to 1.3 μl.16. The incubation segment of claim 15 , wherein the volume of the incubation well Vis greater or equal to 2.2 μl and less than or equal to 2.4 μl claim 15 , the volume of the gas cavity Vis greater or equal to 0.9 μl and less than or equal to 1.2 μl.17. The incubation segment of claim 13 , wherein the volume of the gas cavity Vis greater than or equal to 5% of the volume Vof the ...

MICROFLUIDIC DEVICE

The invention provides a new microfluidic device and method for performing operations on droplets. The invention extends to microfluidic systems comprising one or more of the microfluidic devices. 11001231441144100578578844785578ababab. A microfluidic device () comprising a microfluidic channel () comprising an inlet () and an outlet () , and configured to allow liquid to flow therebetween along a direction of flow , the microfluidic channel () comprising at least one obstruction ( , ) extending thereacross such that the transverse dimension of the microfluidic channel () , as measured in a direction perpendicular to the direction of flow , is less than the transverse dimension of the microfluidic channel () at a point spaced apart from the obstruction ( , ) , the device () further comprising at least one side channel () comprising an inlet () and an outlet () , and configured to allow liquid to flow therebetween , the side channel () being connected to the microfluidic channel by its inlet () and outlet () , such that its outlet () coincides with the obstruction ( , ) , and wherein the lumen of the inlet () and the outlet () of the side channel () is less than the lumen of the side channel () at a position between its inlet () and outlet ().210014411aa. A microfluidic device () according to claim 1 , wherein the transverse dimension of the microfluidic channel () claim 1 , as measured in a first direction perpendicular to the direction of flow claim 1 , is h claim 1 , and the obstruction () comprises a barrier () extending across the channel () claim 1 , wherein the transverse dimension claim 1 , h claim 1 , of the microfluidic channel () claim 1 , as measured in the first direction perpendicular to the direction of flow claim 1 , is h Подробнее

DEVICE FOR SIMULTANEOUS AND UNIFORM THERMAL CYCLING OF SAMPLES AND USES THEREOF

The invention relates to thermal cycling device comprising: a sample location; a first heating means, wherein advantageously said first heating means is a contact heating means; a second heating means, wherein said second heating means is configured to bring said sample to a second temperature by directing electromagnetic radiation to the first light pipe section end and the light pipe section is configured to direct said electromagnetic radiation through its second end to the sample location, its uses and methods based thereon. 1. A thermal cycling device comprising:a sample location;a light source;a light pipe comprising a light pipe section, the light pipe section comprising a first and a second end;a first heater being a contact heat source, wherein said first heater is configured to bring a sample at said sample location to at least about a first temperature;a second heater being a source of electromagnetic radiation, wherein said second heater is configured to simultaneously bring multiple samples at said sample location to a second temperature by directing the electromagnetic radiation to the first light pipe section end and the light pipe section is configured to direct said electromagnetic radiation through its second end to the sample location and homogenize a spatial intensity distribution of the electromagnetic radiation by the reflector; anda camera (configured to directly observe emitted fluorescence from the sample location transmitted by the light pipe section without reflections from sides of the light pipe.2. The thermal cycling device of claim 1 , wherein said camera comprises an objective that only allows the light coming directly from the sample location to pass through.3. The thermal cycling device of claim 1 , wherein said electromagnetic radiation directed to said sample location is configured to simultaneously cause at least 5° C. temperature increase of said multiple samples;advantageously at least 10° C. temperature increase of said ...

Method for performing quantitation assays

The present invention relates to a method for determining an estimate of a concentration of particles E(C), wherein a sample of predetermined volume is divided into a number (N) of compartments, the (N) compartments comprise or consist of different sample volumes (v 1 ) and/or different dilution factors (d i ) of the sample, at least part of the particles that are present in any of the (N) compartments provide a measurable signal and the estimated concentration of particles E(C) is a function of measured signals, as well as an apparatus for use in the inventive method, uses of the inventive method, a sample holder and a kit for use in the inventive method.

Method for performing quantitation assays

The present invention relates to a method for determining an estimate of a concentration of analyte particles E(C) as well as an apparatus for use in the inventive method, uses of the inventive method or the inventive apparatus, a sample holder and a kit for use in the inventive method.

METHOD AND SYSTEM FOR RAPIDLY TESTING ANTIMICROBIAL SUSCEPTIBILITY

Disclosed are methods and systems for rapidly testing antimicrobial susceptibility, wherein the qualitative and quantitative susceptibility of an inoculated microorganism against an antimicrobial agent or a combination of antimicrobial agents is determined as a function of one or more slopes of linear trends α of data of readouts. 1. A computer-implemented method for determining the qualitative or quantitative susceptibility of a microorganism inoculum in a phenotypic antimicrobial susceptibility test (AST) using broth dilution , comprising the following steps:a) Providing a microorganism inoculum and diluting the inoculum in a medium suitable for broth dilution AST,b) Providing a carrier comprising one or more compartments suitable for broth dilution AST, wherein the or at least part of the compartments comprise respectively a single antimicrobial agent or a combination of antimicrobial agents,c) Dispensing a sample of the medium containing the diluted inoculum of step a) into the or at least part of the compartments of the carrier of step b) so that the one or more inoculated compartments of the carrier comprise the respective test assays,d) Incubating the carrier of step c) comprising the respective one or more test assays,{'sub': i', 'i, 'e) Measuring, during the incubation step d), at least n times for each of the one or more test assays with constant or inconstant frequency ƒ a signal derived from a chemical or physical property of the inoculated microorganism, wherein the signal represents an essentially monotonic function of the number of the microorganisms in the measured test assay, and reading out corresponding values {x} at corresponding recording times {t}, wherein i represents the index number of the measurement represented by an integer 1 to n, and wherein n represents a full integer of 13 or more measurements,'}{'sub': i', 'j', 'ij', 'i', 'i', '1, 'f) Estimating one or more slopes of linear trends α in data as a function of distribution of a ...

A microfluidic device and a microfluidic system comprising one or more microfluidic devices

Przedmiotem wynalazku jest urzadzenie mikroprzeplywowe, obejmujace kanal mikroprzeplywowy, posiadajacy wlot i wylot oraz umozliwiajacy przeplyw cieczy w kierunku przeplywu, to jest wzdluz prostej przebiegajacej od wlotu do wylotu kanalu mikroprzeplywowego, którego wymiar poprzeczny, mierzony w pierwszym kierunku prostopadlym do kierunku przeplywu wynosi h1 oraz którego wymiar poprzeczny, mierzony w drugim kierunku prostopadlym do kierunku przeplywu wynosi w1 charakteryzujace sie tym, ze w kanale mikroprzeplywowym (1) znajduje sie przeszkoda w postaci progu (4a), to jest obszaru, gdzie wymiar poprzeczny h2 kanalu mikroprzeplywowego (1) mierzony w pierwszym kierunku prostopadlym do kierunku przeplywu wynosi h2 < h1, lub w postaci zwezenia 4b, to jest obszaru, gdzie wymiar poprzeczny w2 kanalu mikroprzeplywowego (1) mierzony w drugim kierunku prostopadlym do kierunku przeplywu wynosi w2 < w1 oraz urzadzenie posiada co najmniej jeden kanal boczny (5), polaczony z kanalem mikroprzeplywowym (1) poprzez wlot (7) kanalu bocznego (5) i wylot (8) kanalu bocznego (5) oraz polaczony z przeszkoda (4), przy czym swiatlo wlotu (7) kanalu bocznego (5) i wylotu (8) kanalu bocznego (5) jest zmniejszone w porównaniu ze swiatlem kanalu bocznego w miejscu polozonym pomiedzy jego wlotem (7) i wylotem (8), korzystnie o co najmniej 50%, a korzystniej od 66% do 75%, w szczególnosci przez zwezenie lub splycenie kanalu bocznego (5) w obszarze wlotu (7) i wylotu (8) kanalu bocznego. Wynalazek obejmuje takze uklad mikroprzeplywowy, obejmujacy jedno lub wiecej takich urzadzen mikroprzeplywowych. The invention relates to a microfluidic device comprising a microflow channel having an inlet and an outlet and allowing the liquid to flow in the flow direction, that is, along a straight line running from the inlet to the outlet of the microflow channel, the transverse dimension of which is h1 measured in a first direction perpendicular to the flow direction. the transverse dimension, measured in ...

Method and apparatus for fluid dispersion

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.

Microfluidic device for performing operations on microdroplets

The invention provides a microfluidic device and method for performing operations on droplets. The device comprises a microfluidic channel with a side-channel and a barrier. A liquid droplet is introduced in the device and its movement is influenced by the position and dimension of the barrier and the side-channel. The use is comprises metering, merging or trapping small quantities of liquid. The invention extends to microfluidic systems comprising one or more of the microfluidic devices.

Fluid waveguides and uses thereof

The invention relates to methods and apparatuses for guiding and emitting electromagnetic radiation from a fluid waveguide. Various methods for changing optical properties (e.g., refractive index, absorption, and fluorescence) and/or physical properties (e.g., magnetic susceptibility, electrical conductivity, and temperature) of either the waveguide core or the cladding, or both, are provided herein. In one embodiment, electromagnetic radiation is guided and/or emitted at multiple distinct wavelengths, including emission in the form of an essentially continuous band, in some cases covering at least 150 nanometers. In another embodiment, methods for splitting a waveguide core and/or the joining of at least two waveguide cores in a waveguide are provided. In yet another embodiment, the invention includes the use of thermal gradients to generate a waveguide and/or to change the properties of waveguides. Embodiments of the waveguides may be used for optical detection or spectroscopic analysis.

Device for simultaneous and uniform thermal cycling of samples and uses thereof

The invention relates to thermal cycling device comprising: a sample location (4); a first heating means (3), wherein said first heating means is configured to bring a sample at said sample location to at least about a first temperature; preferably said first heating means is a contact heating means; a second heating means (2), wherein said second heating means is configured to bring said sample to a second temperature by directing electromagnetic radiation to the first light pipe section end and the light pipe section is configured to direct said electromagnetic radiation through its second end to the sample location (4), its uses and methods based thereon. The invention also relates to a thermal cycling device comprising: a) a sample location (4); b) a first light pipe section at least operable to collect electromagnetic radiation; c) a second light pipe section at least operable to collect fluorescent excitation and transmit fluorescence emission light; d) a third light pipe section at least operable to illuminate the sample location (4) with electromagnetic radiation and fluorescent excitation and to transmit fluorescent emission light to the second light pipe section; e) an optical filter configured to reflect visible light and transmit electromagnetic radiation; wherein each light pipe section is configured to homogenize spatial intensity distribution of light and electromagnetic radiation by the means of reflection.

Incubation section

Przedmiotem zgłoszenia jest segment inkubacyjny w chipie mikroprzepływowym do oznaczeń mikrobiologicznych, w którym wspomniany segment inkubacyjny jest uformowany w płytce z główną stroną górną oraz główną stroną dolną, wspomniany segment obejmuje komorę inkubacyjną, kanał wlotowy, przez który próbka może być wprowadzona do tej komory inkubacyjnej oraz wnękę gazową połączoną ze wspomnianą komorą inkubacyjną kanałem mikroprzepływowym, w którym wspomniana wnęka nie jest wentylowana, a podstawa wnęki gazowej jest połączona z podstawą komory inkubacyjnej kanałem łączącym. Zgłoszenie obejmuje także chip mikroprzepływowy. The subject of the application is an incubation segment in a microfluidic chip for microbiological determinations, wherein said incubation segment is formed in a plate with a major top side and a major bottom side, said segment comprising an incubation chamber, an inlet channel through which a sample can be introduced into the incubation chamber and a gas cavity connected to said incubation chamber by a microflow conduit, wherein said cavity is not ventilated and the base of the gas cavity is connected to the base of the incubation chamber by a connecting channel. The application also covers a microfluidic chip.

Microfluidic device for fluid dispersion

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-availabe, inexpensive material using simple techniques.

Method for splitting droplets on demand in microfluidic junction

The invention relates to a method for splitting droplets on demand in a microfluidic junction, comprising the supply channel, the first drain channel and the second drain channel, characterised in that it comprises the following stages: a. delivering a droplet (1) to the said microfluidic junction (3) through the said supply channel (2) by means of a flow of continuous liquid through this channel (2) and the said first drain channel, b. stopping the flow in the said first drain channel and opening the flow in the said second drain channel until a fraction (7) of the said droplet (1) is present in the said second drain channel, c. resuming the flow in the said first drain channel and closing the flow in the said second drain channel, at least until the fraction (7) of the said droplet (1) being present in the said first drain channel separates from the rest of the droplet.

Microfluidic chip and valve, production process and uses

The present invention relates to a microfluidic chip and valve, production process and uses thereof according to the independent claims.

Method for determining biochemical parameters of a body fluid

The invention relates to a method for determining biochemical parameters of a body fluid, wherein a sample of said body fluid in the form of a droplet is transported through a channel of a microfluidic system using a carrier liquid, mixed with a reagent thus initiating a chemical reaction between the sample and the reagent, and the result of the chemical reaction is measured, preferably with a spectrophotometer, whereby the said biochemical parameters of the body fluid are determined, characterised in that the material used for fabrication of the microfluidic system and the said carrier liquid are pairs selected from the group comprising: polypropylene and hexadecane, polyethylene and hexadecane, cyclic olefin copolymer 5013 and hexadecane, Teflon and Fluorinert HFE-7100.

Method and apparatus for fluid dispersion

Method of separating paramagnetic material from drops on demand and a system for separating paramagnetic material from drops on demand

Verfahren zur Abtrennung von paramagnetischem Material aus einem Tropfen auf Anforderung zur Abtrennung, umfassend die Verlagerung des das paramagnetische Material enthaltenden Tropfens in einen mikrofluidischen Kanal, wobei ein Elektromagnet in der Nähe des mikrofluidischen Kanals angebracht ist und elektrische Spannung an die Spule des Elektromagneten mindestens zum Zeitpunkt, wenn der Tropfen sich in der Nähe des Elektromagneten befindet, angelegt wird, dadurch gekennzeichnet, dass der mikrofluidische Kanal eine Mulde hat, das heißt einen Abschnitt, in dem die Querschnittfläche des Kanals vergrößert ist, und der Elektromagnet in der Nähe dieser Mulde angebracht wird, dass die Mulde eine Länge von 50% bis 200% der Breite des mikrofluidischen Kanals, vorzugsweise von 75% bis 100% der Breite des mikrofluidischen Kanals, hat, und dass die Mulde eine Breite von 25% bis 100% der Breite des mikrofluidischen Kanals, vorzugsweise von 25% bis 50% der Breite des mikrofluidischen Kanals, hat, und dass das paramagnetische Material durch ein von dem Elektromagneten erzeugtes magnetisches Feld aus dem Tropfen herausgezogen und in der Mulde gehalten wird, nachdem der Tropfen die Mulde passiert hat. A method of separating paramagnetic material from a droplet on demand for separation, comprising displacing the droplet containing the paramagnetic material into a microfluidic channel, wherein an electromagnet is mounted in the vicinity of the microfluidic channel and electrical voltage is applied to the coil of the electromagnet at least at the time when the drop is in the vicinity of the electromagnet, characterized in that the microfluidic channel has a trough, that is, a portion in which the cross-sectional area of the channel is increased, and the electromagnet is mounted in the vicinity of this trough in that the trough has a length of 50% to 200% of the width of the microfluidic channel, preferably 75% to 100% of the width of the microfluidic channel, and that the trough has a ...

Method of dividing drops at a microfluidic junction.

Gegenstand der Erfindung ist ein Verfahren zur Teilung von Tropfen an einer mikrofluidischen Kreuzung, umfassend einen ersten Zufuhrkanal, einen zweiten Zufuhrkanal und einen Abfuhrkanal, gekennzeichnet dadurch, dass es die folgenden Etappen umfasst: a) Zuführung des Tropfens (1) an die mikrofluidische Kreuzung (3) durch den ersten Zufuhrkanal (2) mithilfe einer Strömung einer kontinuierlichen Flüssigkeit durch den ersten Zufuhrkanal (2) und den Abfuhrkanal, b) Einstellung der Strömung in dem ersten Zufuhrkanal (2) zu dem Zeitpunkt, wenn sich der Tropfen (1) im Lumen des zweiten Zufuhrkanals befindet, c) Öffnung der Strömung der kontinuierlichen Flüssigkeit in dem zweiten Zufuhrkanal bis zum Zeitpunkt, wenn der Tropfen (1) in zwei Teile zerteilt ist. The invention relates to a method for dividing drops at a microfluidic junction comprising a first feed channel, a second feed channel and a discharge channel, characterized in that it comprises the following stages: a) feeding the drop (1) to the microfluidic junction ( 3) through the first supply channel (2) by means of a flow of a continuous liquid through the first supply channel (2) and the discharge channel, b) adjusting the flow in the first supply channel (2) at the time when the drop (1) in the Lumen of the second supply channel is c) opening the flow of the continuous liquid in the second supply channel until the time when the droplet (1) is divided into two parts.

The method of dividing droplets into a task in a microfluidic connector

Dividing drops at a microfluidic junction comprising a first supply channel, a second supply channel and a discharge channel, comprises (a) supplying the drops (1) to the microfluidic junction (3) through the first supply channel (2) by flowing continuous liquid through the channel and the discharge channel, (b) adjusting the flow in the first supply channel to a time point, at which the drops are present in a lumen of the second supply channel, and (c) opening the flow of the continuous liquid in the second supply channel up to a time point, at which the drops are divided into two portions.

method for performing quantitation tests

resumo patente de invenção: "método para realizar ensaios de quantificação". a presente invenção refere-se a um método para determinar uma estimativa da concentração de moléculas de analito ou partículas e(c) em que uma amostra de volume pré-determinado é dividida em um número (n) de compartimentos, os (n) compartimentos compreendem ou consistem de diferentes volumes de amostra (v_i) e/ou diferentes fatores de diluição (d_i) da amostra, pelo menos parte das moléculas de analito ou partículas que estão presentes em qualquer dos (n) compartimentos fornecem um sinal mensurável e a concentração estimada de moléculas ou partículas e(c) é uma função dos sinais medidos, assim como um aparelho para uso no método da invenção, usos do método da invenção, um suporte de amostra e um kit para uso no método da invenção. patent summary: "method for performing quantitation assays". The present invention relates to a method for determining an estimate of the concentration of analyte molecules or particles and (c) wherein a predetermined volume sample is divided into a number (n) of compartments, the (n) compartments. comprise or consist of different sample volumes (v_i) and / or different dilution factors (d_i) of the sample, at least part of the analyte molecules or particles that are present in any of the (n) compartments provide a measurable signal and the concentration Estimated number of molecules or particles and (c) is a function of the measured signals, as is an apparatus for use in the method of the invention, uses of the method of the invention, a sample holder and a kit for use in the method of the invention.

Securing inner surface of polycarbonate microfluidic channel, against action of organic solvents and polycarbonate surface, by treating surface with three types of modifying solutions and/or heating the surface at an elevated temperature

The method comprises treating a surface sequentially with three types of modifying solutions and/or heating the surface at an elevated temperature, additionally treating the surface with a fourth modifying solution, and washing the surface with a perfluorinated solvent such as a mixture of methoxy nonafluorobutane and methoxy nonafluoro-isobutane including HFE-7100 and perfluorotripropylamine such as FC-3283 and with isopropanol and then drying at a temperature of 100[deg] C. The second modifying solution contains an organic epoxy-containing compound. The method comprises treating a surface sequentially with three types of modifying solutions and/or heating the surface at an elevated temperature, additionally treating the surface with a fourth modifying solution, and washing the surface with a perfluorinated solvent such as a mixture of methoxy nonafluorobutane and methoxy nonafluoro-isobutane including HFE-7100 and perfluorotripropylamine such as FC-3283 and with isopropanol and then drying at a temperature of 100[deg] C. The second modifying solution contains an organic epoxy-containing compound, where the groups of compound are connected by covalent bonds with amino groups in the polymer chains of the polymer contained in the first modifying solution such as trimethylolpropane triglycidyl ether and/or a single aliphatic alcohol. The polycarbonate surface is treated with the first, second and/or third modifying solution at a temperature of 70[deg] C for a time period of 1 hour. The surface of the polycarbonate microfluidic channel is treated with the first, second and/or third modifying solution so that the solution is fed through the channel, where a linear velocity of the first, second and/or the third modifying solution by the microfluidic channel is 26 cm/min. The fourth modifying solution is a compound containing an acid chloride group and perfluorinated polymer chain, and is capable of forming an amide bond with amino groups. The polycarbonate surface is ...

Microfluidic chip

system and method for automated production and handling of liquid mixtures

Microfluidic circuit, microfluidic chip, kit and method for isolating and purifying an analyte from a biologic sample

The present invention relates to a microfluidic circuit, microfluidic chip, kit and method for isolating and purifying an analyte, preferably a nucleic acid, according to the independent claims.

Method and apparatus for fluid dispersion

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.

Microfluidic chip

Device for simultaneous and uniform thermal cycling of samples and uses thereof

Method for performing quantitation assays

The present invention relates to a method for determining an estimate of a concentration of analyte particles E(C) as well as an apparatus for use in the inventive method, uses of the inventive method or the inventive apparatus, a sample holder and a kit for use in the inventive method.

Microfluidic chip, production process and uses

The present invention relates to a microfluidic chip for partitioning a liquid composition into multiple aliquots, production processes and uses thereof according to the independent claims.

Sposób dostarczania próbki plynu ustrojowego do ukladu analizujacego

Sposób dostarczania próbki plynu ustrojowego do ukladu analizujacego obejmujacy etapy pobrania plynu ustrojowego do komory ukladu mikroprzeplywowego, odwirowania plynu ustrojowego w komorze ukladu mikroprzeplywowego charakteryzuje sie tym, ze nastepnie dokonuje sie wypchniecia plynu ustrojowego z komory (4) ukladu mikroprzeplywowego do ukladu analizujacego.

Mikroprzepływowy układ zwłaszcza do automatycznego tworzenia dwuwarstw fosfolipidowych i badania aktywności białek błonowych

Sposób modyfikacji powierzchni poliwęglanu i powierzchnia poliwęglanu zmodyfikowana tym sposobem

Method and system for rapidly testing antimicrobial susceptibility

Disclosed are methods and systems for rapidly testing antimicrobial susceptibility, wherein the qualitative and quantitative susceptibility of an inoculated microorganism against an antimicrobial agent or a combination of antimicrobial agents is determined as a function of one or more slopes of linear trends α of data of readouts.

Urządzenie do jednoczesnego i jednolitego cyklicznego zmieniania temperatury próbek i jego zastosowania

Dispositivo para ciclado térmico simultáneo y uniforme de muestras y usos del mismo

La invención se refiere a un dispositivo de ciclado térmico que comprende: una ubicación de muestra (4); un primer medio de calentamiento (3), en el que dicho primer medio de calentamiento está configurado para llevar una muestra en dicha ubicación de muestra a al menos aproximadamente una primera temperatura; preferiblemente dicho primer medio de calentamiento es un medio de calentamiento por contacto; un segundo medio de calentamiento (2), en el que dicho segundo medio de calentamiento está configurado para llevar dicha muestra a una segunda temperatura dirigiendo radiación electromagnética al extremo de la primera sección de tubo de luz y la sección de tubo de luz está configurada para dirigir dicha radiación electromagnética a través de su segundo extremo al lugar de la muestra (4), sus usos y métodos basados en el mismo. La invención también se refiere a un dispositivo de ciclado térmico que comprende: a) una ubicación de muestra (4); b) una primera sección de tubo de luz que puede funcionar al menos para recoger radiación electromagnética; c) una segunda sección de tubo de luz al menos operable para recoger excitación fluorescente y transmitir luz de emisión fluorescente; d) una tercera sección de tubo de luz al menos operable para iluminar la ubicación de la muestra (4) con radiación electromagnética y excitación fluorescente y para transmitir luz de emisión fluorescente a la segunda sección de tubo de luz; e) un filtro óptico configurado para reflejar la luz visible y transmitir radiación electromagnética; en el que cada sección de tubo de luz está configurada para homogeneizar la distribución de intensidad espacial de la luz y la radiación electromagnética mediante reflexión. (Traducción automática con Google Translate, sin valor legal)

Microfluidic systems, in particular for automatic generation of phospholipid bilayers and for testing protein activity in membranes

Sposób automatycznego tworzenia dwuwarstw fosfolipidowych i/lub badania aktywności białek błonowych i/lub regulacji stężenia białek błonowych

Mikroprzepływowy układ zwłaszcza do automatycznego tworzenia dwuwarstw fosfolipidowych i badania aktywności białek błonowych

Mikroprzepływowy układ zwłaszcza do automatycznego tworzenia dwuwarstw fosfolipidowych i badania aktywności białek błonowych

Przedmiotem wynalazku jest mikroprzepływowy układ, zwłaszcza do automatycznego tworzenia dwuwarstw fosfolipidowych i badania aktywności białek błonowych, obejmujący pułapkę hydrodynamiczną (1) w postaci komory na płyn, płytszej przy ścianach komory i głębszej z dala od ścianek komory, która to komora posiada pierwszą część i drugą część, korzystnie będące wzajemnie swoimi lustrzanymi odbiciami, z przewężeniem ukształtowanym pomiędzy pierwszą częścią a drugą częścią, przy czym każda z części komory przeznaczona jest na dwie krople płynu, w którym to układzie pierwszy kanał mikroprzepływowy (14) połączony z pierwszą częścią komory pułapki hydrodynamicznej (1) przeznaczony jest do doprowadzania kropel do pierwszej części komory, zaś drugi kanał mikroprzepływowy (15) połączony z drugą częścią komory pułapki (1) przeznaczony jest do doprowadzania kropel do drugiej części komory, który charakteryzuje się tym, że posiada dwie pary kanałów (21 i 23), (22 i 24), przeznaczonych do wyprowadzania kropel z układu, przy czym jedna para kanałów (21, 23) prowadzi do pierwszej części komory pułapki (1), zaś druga para kanałów (22, 24) prowadzi do drugiej części komory pułapki (1) oraz że każda spośród pierwszej części komory pułapki (1) i drugiej części komory pułapki (1) obejmuje dwie przestrzenie na płyn z przewężeniem ukształtowanym między tymi przestrzeniami, przy czym w pierwszej części komory pułapki (1) kanały jednej pary kanałów (21, 23) prowadzą do różnych przestrzeni, zaś w drugiej części komory pułapki (1) kanały (22, 24) prowadzą do różnych przestrzeni.

Mikroprzepływowy układ zwłaszcza do automatycznego tworzenia dwuwarstw fosfolipidowych i badania aktywności białek błonowych

Przedmiotem wynalazku jest mikroprzepływowy układ, zwłaszcza do automatycznego tworzenia dwuwarstw fosfolipidowych i badania aktywności białek błonowych, obejmujący pułapkę hydrodynamiczną w postaci komory na płyn, płytszej przy ścianach komory i głębszej z dala od ścianek komory, która to komora posiada pierwszą część i drugą część, korzystnie będące wzajemnie swoimi lustrzanymi odbiciami, z przewężeniem ukształtowanym pomiędzy pierwszą częścią a drugą częścią, przy czym każda z części przeznaczona jest na kroplę płynu, charakteryzujący się tym, że ponadto posiada: a) pierwszy kanał mikroprzepływowy, połączony z pierwszą częścią komory pułapki (1), przeznaczony do doprowadzania kropel do pierwszej części komory oraz drugi kanał mikroprzepływowy, połączony z drugą częścią komory pułapki (1), przeznaczony do doprowadzania kropel do drugiej części komory, b) parę kanałów (14), przeznaczonych do wyprowadzania kropel z układu, przy czym jeden z kanałów (14) prowadzi do pierwszej części komory pułapki (1), zaś drugi z kanałów (14) prowadzi do drugiej części komory pułapki (1).

Mikronestejärjestelmä, erityisesti fosfolipidikaksoiskerrosten automattiseen generointiin ja kalvon proteiiniaktiivisuuden testaamiseksi

Mikroprzepływowy układ zwłaszcza do automatycznego tworzenia dwuwarstw fosfolipidowych o zmiennej i kontrolowanej wielkości oraz jego zastosowanie

Przedmiotem wynalazku jest mikroprzepływowy układ, zwłaszcza do automatycznego tworzenia dwuwarstw fosfolipidowych i badania aktywności białek błonowych, obejmujący pułapkę hydrodynamiczną w postaci komory na płyn, płytszej przy ścianach komory i głębszej z dala od ścianek komory, która to komora posiada pierwszą część i drugą część, korzystnie będące wzajemnie swoimi lustrzanymi odbiciami, z przewężeniem ukształtowanym pomiędzy pierwszą częścią a drugą częścią, przy czym każda z części przeznaczona jest na kroplę płynu, a ponadto posiadający: a) pierwszy kanał mikroprzepływowy, połączony z pierwszą częścią komory pułapki, przeznaczony do doprowadzania kropel do pierwszej części komory oraz drugi kanał mikroprzepływowy, połączony z drugą częścią komory pułapki, przeznaczony do doprowadzania kropel do drugiej części komory, b) parę kanałów, przeznaczonych do wyprowadzania kropel z układu, przy czym jeden z kanałów prowadzi do pierwszej części komory pułapki, zaś drugi z kanałów prowadzi do drugiej części komory pułapki, c) dodatkowy kanał mikroprzepływowy, służący do rozdzielania kropel w trakcie ich wymiany w pułapce lub do ich odsuwania, położony poza pułapką, przy czym wyloty dodatkowego kanału mikroprzepływowego znajdują się po przeciwległych stronach pułapki, w miejscu przewężenia między pierwszą częścią komory pułapki a drugą częścią komory pułapki, charakteryzujący się tym, że wyloty dodatkowego kanału mikroprzepływowego (13) znajdujące się w miejscu przewężenia między pierwszą częścią komory pułapki (1) a drugą częścią komory pułapki (1) przebiegają w kierunku normalnym do komory pułapki (1) i są ukształtowane tak, że w warunkach pracy układu wyloty kanału (13) umieszczone są równolegle do powierzchni błony na styku kropel. Wynalazek obejmuje także zastosowanie takiego układu mikroprzepływowego, zwłaszcza do automatycznego tworzenia dwuwarstw fosfolipidowych, badania aktywności białek błonowych i/lub do regulacji stężenia białek błonowych.

Strzykawka, zestaw do dostarczania surowicy krwi zawierajacy taka strzykawke oraz sposób dostarczania surowicy krw, wykorzystujacy taka strzykawke

Strzykawka, obejmujaca komore przeznaczona na krew, ograniczona powierzchnia boczna, posiadajaca na pierwszym koncu wylot, zas na drugim koncu zamknieta tloczkiem, charakteryzuje sie tym, ze w powierzchni bocznej komory (1) znajduje sie otwór (2), przy czym odleglosc otworu (2) od drugiego konca komory (1) jest mniejsza niz dlugosc tloczka (3), dzieki czemu przez wcisniecie tloczka (3) do komory (1) mozna calkowicie zaslonic otwór (2) tloczkiem (3). Wynalazek obejmuje takze zestaw do dostarczania surowicy krwi, obejmujacy taka strzykawke, dysze mikroprzeplywowa (5), dopasowana do wylotu komory (1) strzykawki, oraz rotor wirówki, posiadajacy zaglebienie, sluzace do polozenia w nim strzykawki z zamocowana dysza (5), dopasowane do wielkosci i ksztaltu strzykawki z dysza (5). Wynalazek dotyczy takze sposobu dostarczania surowicy krwi, obejmujacego: nabór krwi do takiej strzykawki przez otwór (2), umieszczenie strzykawki z krwia w rotorze wirówki i odwirowanie krwi w strzykawce, wprowadzenie surowicy ze strzykawki poprzez dysze mikroprzeplywowa (5) do analizatora.

Verfahren zur Modifizierung einer Polycarbonat-Oberfläche.

Der Gegenstand der Erfindung ist ein Verfahren zur Modifizierung einer Polycarbonat-Oberfläche, umfassend eine Stufe zur Einwirkung einer ersten Modifizierungslösung auf die Oberfläche sowie eine Stufe zur Einwirkung einer zweiten Modifizierungslösung auf die Oberfläche, das sich dadurch auszeichnet, dass die erste Modifizierungslösung ein Polymer, umfassend Polymerketten mit einer Aminogruppe, beinhaltet, und die zweite Modifizierungslösung ein Polyelektrolyt, umfassend Moleküle, die sich durch eine lonenbindung mit den Polymerketten des Polymers verbinden können, beinhaltet.

Sposób wytwarzania kapsułek z hydrofilowym rdzeniem i polimerową otoczką

Microfluidic system

The invention relates to a microfluidic system for fluid specimen collection, especially blood, by means of capillary forces, and separation thereof by centrifugation, where the second end (8) of the vent channel (6) is directly connected to the capillary aspiration channel (4) inside the system, in the section between the inlet of the aspiration channel (5) and the first chamber (1), and where the first chamber (1) is structurally adapted so that it can be accessed by means of a standard tip for specimen collection, for example a needle or an automatic pipette tip, and that it is possible to collect the content from the inside of the first chamber (1).

Microfluidic circuit, microfluidic chip, kit and method for isolating and purifying an analyte from a biologic sample

Disclosed is a microfluidic circuit, microfluidic chip, kit and method for isolating and purifying an analyte, preferably a nucleic acid.

Kapsułki z hydrofilowym rdzeniem i polimerową otoczką i sposób ich wytwarzania

Microfluidic chip

A microfluidic chip for conducting microbiological assays, includes a substrate in which incubation segments, a sample reservoir and microfluidic channels connecting the sample reservoir with the incubation segments are arranged. The microfluidic chip further includes a non-aqueous liquid reservoir for containing non-aqueous liquid wherein the reservoir is connectable via a releasable airtight and liquid-tight valve with the microfluidic channels connecting the sample reservoir with the incubation segments each incubation segment comprises an incubation well connected by a gas-exchange channel to an unvented gas cavity.

Układ mikroprzepływowy do wytwarzania monodyspersyjnych kropli

Układ mikroprzepływowy do wytwarzania monodyspersyjnych kropli obejmujący: a. kanał główny, stanowiący zamknięty ściankami bocznymi przewód, posiadający wlot, przeznaczony do łączenia ze źródłem płynu i wylot, przeznaczony do łączenia z rezerwuarem, stanowiącym zbiornik, którego co najmniej jeden poprzeczny wymiar wewnętrzny jest znacznie większy od maksymalnego poprzecznego wymiaru wewnętrznego kanału głównego, korzystnie przynajmniej 2 razy większy, korzystniej nie mniej niż 6 razy większy, b. stopień, stanowiący końcowy odcinek kanału głównego w obrębie którego powstają krople, charakteryzujący się tym, że kanał główny obejmuje co najmniej dwa połączone ze sobą sąsiadujące kanały zwane żłobieniami, o tych samych wymiarach i kształcie przekroju poprzecznego a ponadto oddzielone ściankami bocznymi, których wysokość jest wysokością (H) kanału głównego czyli odległością pomiędzy sufitem i podłogą kanału głównego, a ponadto w obrębie stopnia o długości 0,5 - 2 mm, korzystnie 1 - 2 mm, korzystniej 2 mm, znajduje się prześwit rozciągający się na całej szerokości kanału głównego i stanowiący obszar wspólny wszystkich żłobień, rozciągający się od sufitu kanału głównego na głębokość (h), przy czym wysokość ścianek bocznych żłobień stanowi różnicę pomiędzy wysokością (H) kanału głównego i głębokością (h), gdzie 0 < h < 100% wysokości (H).