Primer, method and kit for carrying out real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection on novel endovirus

The invention discloses a primer, a method and a kit for carrying out real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection on a novel endovirus. The specific sequences of the primers are as follows: Nairo-qP F: 5 '-TGTG CCTTACAGGATTG CCTT-3', Nairo-qP F: 5 '-TGTG CCTTA, Nairo-qP F And the Nairo-qP R is 5 '-TCAAGGTTCTGTTGGTGCCA-3', and the Nairo-qP R is 5 '- Specific primers are designed and synthesized according to a gene sequence of an S fragment of the novel endovirus, a recombinant plasmid standard substance is constructed, a real-time fluorescent quantitative PCR detection system aiming at the novel endovirus is established, quantitative detection of the novel endovirus can be realized, and non-specific amplification of various viruses such as HSV can be realized; the kit can detect 10 < 2 > copies/[mu] L of recombinant plasmids, which is 10000 times of that of conventional PCR, and has the advantages of high specificity and sensitivity, good repeatability, short ...