25-03-2021 дата публикации
Номер: US20210085809A1
The present invention relates to a vaccine composition for use in in vivo administration, comprising a (attenuated) pathogen or commensal modified to be a pre-targeting vector, the pre-targeting vector comprising one or more pendent reactive moieties able to form a high affinity interaction with a complementary moiety residing on an immunogenic conjugate component. 1. A two component vaccine composition for use in in vivo administration , comprising a (preferably attenuated) pathogen or commensal modified as a pre-targeting vector , the pre-targeting vector comprising one or more pendent reactive moieties able to form a high affinity interaction with a complementary conjugate moiety residing on an immunogenic secondary component.2. A The composition of claim 1 , wherein the high affinity interaction comprises a supramolecular vector-conjugate interaction in an inclusion affinity complex.3. The composition of claim 1 , wherein a primary moiety on the vector surface is selected from the group consisting of streptavidin claim 1 , cyclodextrin claim 1 , antibodies claim 1 , antibody fragments claim 1 , ligands and aptamers claim 1 , and wherein the conjugate moiety is a moiety complementary to the respective vector moiety.4. The composition of claim 3 , wherein the vector moiety comprises adamantane groups claim 3 , and wherein the conjugate moiety includes cyclodextrin groups.5. The composition of claim 1 , wherein the high affinity interaction comprises formation of one or more covalent bonds.6. The composition of claim 5 , wherein the pathogen or commensal is functionalised to form a covalent click connection with a conjugate moiety claim 5 , preferably using copper-free click chemistry.7. The composition of claim 5 , wherein the vector moiety comprises one or more azide groups claim 5 , and wherein the conjugate moiety linked to the pathogen comprises one or more reactive alkyne groups claim 5 , preferably wherein both groups are suited for copper-free click ...
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