Thermophilic microorganisms for ethanol production

THERMOPHILIC MICRO-ORGANISMS FOR ETHANOL PRODUCTION

A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.

THERMOPHILIC MICROORGANISMS FOR ETHANOL PRODUCTION

A thermophilic microorganism is modified to permit the increased production of ethanol, wherein a first modification is the inactivation of the lactate dehydrogenase gene and a second modification upregulates the pyruvate dehydrogenase gene. © KIPO & WIPO 2009 ...

THERMOPHILIC MICROORGANISMS FOR ETHANOL PRODUCTION

A thermophilic microorganism is modified to permit the increased producti on of ethanol, wherein a first modification is the inactivation of the lacta te dehydrogenase gene and a second modification upregulates the pyruvate deh ydrogenase gene.

Thermophilic microorganisms for ethanol production

A thermophilic microorganism is modified to permit the increased production of ethanol, wherein a first modification is the inactivation of the lactate dehydrogenase gene and a second modification upregulates the pyruvate dehydrogenase gene.

Thermophilic microorganisms with inactivated lactate dehydrogenase gene (IDH) for ethanol production

Thermophilic microorganisms with inactivated lactate dehydrogenase gene (LDH) for ethanol production

Modified microorganisms with inactivated lactate dehydrogenase gene

Modified microorganisms with inactivated lactate dehydrogenase gene

MODIFIED MICROORGANISMS WITH INACTIVATED LACTATE DEHYDROGENASE GENE

Modified microorganisms are prepared by inactivation of the endogenous lactate dehydrogenase gene. The microorganisms are deposited under NCIMB Accession Nos. 41277, 41278, 41279, 41280 and 41281. © KIPO & WIPO 2008 ...

THERMOPHILIC MICROORGANISMS WITH INACTIVATED LACTATE DEHYDROGENASE GENE (LDH) FOR ETHANOL PRODUCTION

A mutated thermophilic microorganism is prepared, with a modification to inactivate the lactate dehydrogenase gene of a wild-type microorganism. The mutated microorganism is used in the production of ethanol, utilising C3, C5 or C6 sugars as the substrate. © KIPO & WIPO 2008 ...

Thermophilic micro-organisms for ethanol production

A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.

Thermophilic microorganisms with inactivated lactate dehydrogenase gene(LDH) for ethanol production

Thermophilic micro-organisms for ethanol production

A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.

Modified Microorganisms with Inactivated Lactate Dehydrogenase Gene

Modified microorganisms are prepared by inactivation of the endogenous lactate dehydrogenase gene. The microorganisms are deposited under NCIMB Accession Nos. 41277, 41278, 41279, 41280 or 41281.

Thermophilic microorganisms for ethanol production

A thermophilic microorganism is modified to permit the increased production of ethanol, wherein a first modification is the inactivation of the lactate dehydrogenase gene and a second modification upregulates the pyruvate dehydrogenase gene.

Modified microorganisms with inactivated lactate dehydrogenase gene

Modified microorganisms are prepared by inactivation of the endogenous lactate dehydrogenase gene. The microorganisms are deposited under NCIMB Accession Nos. 41277, 41278, 41279, 41280 or 41281.

Thermophilic micro-organisms for ethanol production

A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.

THERMOPHILIC MICROORGANISMS FOR ETHANOL PRODUCTION

A thermophilic microorganism is modified to permit the increased production of ethanol, wherein a first modification is the inactivation of the lactate dehydrogenase gene and a second modification upregulates the pyruvate dehydrogenase gene.

Thermophilic micro-organisms for ethanol production

A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.

THERMOPHILIC MICRO-ORGANISMS FOR ETHANOL PRODUCTION

A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.

THERMOPHILIC MICRO-ORGANISMS FOR ETHANOL PRODUCTION

A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.

THERMOPHILIC MICRO-ORGANISMS FOR ETHANOL PRODUCTION

A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene. 1. A thermophilic micro-organism modified by the insertion of a heterologous amylase gene under the control of a suitable promoter , and further modified to inactivate the native lactate dehydrogenase (ldh) gene.2. The micro-organism according to claim 1 , wherein the promoter operates in low aeration or anaerobic conditions.3. The micro-organism according to claim 1 , wherein the promoter is a heterologous ldh promoter.4Geobacillus stearothermophilus.. The micro-organism according to claim 3 , wherein the ldh promoter is derived from5. The micro-organism according to claim 1 , wherein the amylase gene is under the control of a series of strong promoters including a glyceraldehyde-3-phosphate dehydrogenase promoter or an amylase promoter.6G. stearothermophilus.. The micro-organism according to claim 5 , wherein the promoters are derived from7. The micro-organism according to claim 1 , wherein the lactate dehydrogenase gene claim 1 , or a portion thereof claim 1 , has been deleted.8. The micro-organism according to claim 1 , wherein the micro-organism does not comprise an insertion element in the lactate dehydrogenase gene.9. The micro-organism according to claim 1 , wherein a further modification inactivates the native pyruvate formate lyase gene.10. The micro-organism according to claim 1 , further comprising a modification that up-regulates pyruvate decarboxylase activity.11. The micro-organism according to claim 1 , wherein the dihydrolipoamide transacetylase gene claim 1 , or a portion thereof claim 1 , is deleted.12Geobacillus stearothermophilus.. The micro-organism according to claim 1 , wherein the amylase gene is derived from13Geobacillus thermoglucosidasius.. The micro-organism according to claim 1 , wherein the micro-organism is14. The micro-organism ...

Multi-Stage Equalisation Method and Apparatus for Use in Telemetry

The present invention relates to inspection apparatus for use in wellbores in the oil and gas industries. In particular the invention relates in general to the field of transmission of data between downhole module in a wellbore and a controlling module at the surface. The invention provides a method and apparatus for determining analogue filter parameters for an analogue front end comprising a plurality of filter stages receiving signals from a telemetry module, by repeating the steps of; receiving a signal of a known frequency and processing said signal by determining the magnitude of the frequency of the received signal until a plurality of signals have been received and processed; calculating an optimum set of filter parameters in dependence upon the measured frequency magnitudes and a predefined set of filter stage frequency responses. 1. A method of initialising a surface modem comprising a controller coupled to an analogue front end comprising a plurality of filter stages for receiving signals from a telemetry module , the method comprising the steps of: receiving a signal of a known frequency; and', 'processing said signal by determining the magnitude of the frequency of the received signal;, 'repeating the steps of;'}until a plurality of signals have been received and processed;calculating an optimum set of filter parameters in dependence upon the measured frequency magnitudes and a predefined set of filter stage frequency responses and setting the filter parameters of the analogue front end to said set of optimum filter parameters.2. A method according to claim 1 , in which the optimum set of filter parameters are determined by:for each of a set of filter parameters and each of a predetermined frequency;determining an observed frequency response in dependence upon the magnitude of the frequency of the observed signal and determining a resulting frequency response in dependence upon the observed frequency response and a cumulative filter frequency response; ...

Multi-Stage Equalisation Method and Apparatus for Use in Telemetry

An inspection apparatus for use in wellbores in the oil and gas industries relates in general to the field of transmission of data between downhole module in a wellbore and a controlling module at the surface. The invention provides a method and apparatus for determining analogue filter parameters for an analogue front end comprising a plurality of filter stages receiving signals from a telemetry module, by repeating the steps of; receiving a signal of a known frequency and processing said signal by determining the magnitude of the frequency of the received signal until a plurality of signals have been received and processed; calculating an optimum set of filter parameters in dependence upon the measured frequency magnitudes and a predefined set of filter stage frequency responses.

Inspection assembly viewport

Inspection assemblies that include one or more sideview cameras for capturing images of an interior surface of a pipe or conduit include an elongate housing, a sideview camera within the housing and arranged to capture an image of a region within a field of view external to the housing, and a viewport element mounted in the housing and located such that light is transmitted through the viewport element and into said sideview camera, wherein the viewport element is diffusion bonded to a metal support.

INSPECTION ASSEMBLY VIEWPORT

Inspection assemblies that include one or more sideview cameras for capturing images of an interior surface of a pipe or conduit include an elongate housing, a sideview camera within the housing and arranged to capture an image of a region within a field of view external to the housing, and a viewport element mounted in the housing and located such that light is transmitted through the viewport element and into said sideview camera, wherein the viewport element has a concave internal surface, closer to the camera, and a convex external surface, further from the camera, and wherein the centre of the radius of curvature of the internal surface is closer to the viewport element than the centre of the radius of curvature of the external surface. 1. An inspection assembly for imaging the internal surface of a pipe or conduit comprising:an elongate housing;a sideview camera within the housing and arranged to capture an image of a region within a field of view external to the housing; anda viewport element mounted in the housing and located such that light is transmitted through the viewport element and into said sideview camera,wherein the viewport element has a concave internal surface, closer to the camera, and a convex external surface, further from the camera, and wherein the centre of the radius of curvature of the internal surface is closer to the viewport element than the centre of the radius of curvature of the external surface.2. An inspection assembly as claimed in claim 1 , wherein the viewport element is made of sapphire.3. An inspection assembly as claimed in claim 1 , wherein the sideview camera is mounted such that the centre line of the field of view extends in a direction substantially perpendicular to a longitudinal axis of the housing.4. An inspection assembly as claimed in claim 1 , wherein the housing is substantially cylindrical.5. An inspection assembly as claimed in claim 1 , further comprising a plurality of sideview cameras.6. An inspection ...

Inspection assembly lighting system

The field of inspection assemblies and in particular to inspection assemblies that include light sources for illuminating a field of view of a camera including an elongate housing having a longitudinal axis, a camera mounted in the housing and arranged to capture an image of a region within a field of view external to the housing, a light source mounted in the housing and arranged to illuminate the field of view, and a window element mounted in the housing, the window element comprising a light transmitting material and being located such that light emitted by the light source passes through the window element before illuminating the field of view. The window element has an internal surface, closer to the light source, and an external surface, further from the light source, and the external surface comprises a concave region.

NOVEL ANTIBIOTIC COMPOUND AND METHODS FOR PREPARING THE SAME

L'INVENTION CONCERNE UN NOUVEAU COMPOSE REPONDANT A LA FORMULE I CI-DESSOUS. CE COMPOSE POSSEDE UNE ACTIVITE ANTIPARASITE ET CONSTITUE UN INTERMEDIAIRE INTERESSANT POUR LA PREPARATION D'AUTRES COMPOSES A ACTIVITE ANTIBIOTIQUE. L'INVENTION CONCERNE EN OUTRE DES PROCEDES POUR LA PREPARATION DU COMPOSE DE LA FORMULE I.

microorganisms modified with inactivated lactate dehydrogenase gene

Microorganismos modificados são preparados por inativação do gene endógeno da lactato desidrogenase. Os microorganismos são depositados sob os n~os~. de acesso NOIMB 41277, 41278, 41279, 41280 e 41281. Modified microorganisms are prepared by inactivation of the endogenous lactate dehydrogenase gene. The microorganisms are deposited under nos. access codes 41277, 41278, 41279, 41280 and 41281.

Cyclic ketal derivatives

Compounds are described of formula (I) wherein R1 represents a hydrogen atom or an acetyl group; R2, R3 and R4 may each independently represent a hydrogen atom or a methyl group; n represents an integer from 1 to 3; and the fluorine atom(s) present may be attached at the ortho, meta or para position of the benzene ring relative to the rest of the molecule; and salts thereof. These compounds inhibit the enzyme squalene synthase and/or are intermediates for the preparation of compounds which inhibit the enzyme squalene synthase. Compounds of the invention may be formulated for use in a variety of conditions where a lowering of the level of blood plasma cholesterol in animals would be beneficial and for use in combating fungal infections in animals.

Modified microorganisms with inactivated lactate dehydrogenase gene

Modified microorganisms are prepared by inactivation of the endogenous lactate dehydrogenase gene. The microorganisms are deposited under NCIMB Accession Nos. 41277, 41278, 41279, 41280 and 41281.

Inspection assembly viewport

Multi-stage equalisation method and apparatus for use in telemetry

Inspection assembly viewport

This invention relates to inspection assemblies that include one or more sideview cameras for capturing images of an interior surface of a pipe or conduit. This invention also relates to downhole inspection tools including such inspection assemblies. An inspection assembly (110) comprises an elongate housing (116), a sideview camera within the housing (116) and arranged to capture an image of a region within a field of view external to the housing (116), and a viewport element (114) mounted in the housing (116) and located such that light is transmitted through the viewport element (114) and into said sideview camera, wherein the viewport element (114) has a concave internal surface (156), closer to the camera, and a convex external surface (158), further from the camera, and wherein the centre of the radius of curvature of the internal surface (156) is closer to the viewport element (114) than the centre of the radius of curvature of the external surface (158).

Inspection assembly viewport

Thermophilic micro-organisms for ethanol production

A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.

Thermophilic microorganisms for ethanol production

A thermophilic microorganism is modified to permit the increased production of ethanol, wherein a first modification is the inactivation of the lactate dehydrogenase gene and a second modification upregulates the pyruvate dehydrogenase gene.

Process for the preparation of macrolide compounds

THERMOPHILIC MICROORGANISMS FOR ETHANOL PRODUCTION

Thermophilic microorganisms for ethanol production

A thermophilic microorganism is modified to permit the increased production of ethanol, wherein a first modification is the inactivation of the lactate dehydrogenase gene and a second modification upregulates the pyruvate dehydrogenase gene.

Thermophilic micro-organisms for ethanol production

Composto antibiotico, composizioni che lo contengono e relativo metodo di preparazione e applicazione

Multi-spectral imaging system and method for conduits

A method of hyperspectral imaging an internal surface of a conduit comprises deploying an inspection assembly 10 within an internal volume of a conduit, the inspection assembly comprising a camera/image sensor 24 and a lighting array 30a, 30b including a plurality of light emitters 32; illuminating a region of interest on said internal surface using the lighting array; and capturing image data of the region of interest using the camera, the image data comprising spatial data and intensity data at a plurality of different narrowband wavelength ranges of light. Each lighting array may comprise a plurality of annular sub-arrays 34 each operating at a different wavelength. LEDs may be used or a wideband light source with filters. Alternatively, a forward-facing camera 120 and lighting array 122 may be used (Fig 4). The inspection assembly may detect different defects on the interior of pipelines, such as different corrosion types.

Inspection assembly lighting system

This invention relates to the field of inspection assemblies and in particular to inspection assemblies that include light sources for illuminating a field of view of a camera. An inspection assembly for imaging the internal surface of a pipe or conduit comprises an elongate housing having a longitudinal axis, a camera mounted in the housing and arranged to capture an image of a region within a field of view external to the housing, a light source mounted in the housing and arranged to illuminate the field of view, and a window element mounted in the housing, the window element comprising a light transmitting material and being located such that light emitted by the light source passes through the window element before illuminating the field of view. The window element has an internal surface, closer to the light source, and an external surface, further from the light source, and the external surface comprises a concave region.

Belysningssystem til inspektionsenhed

Inspection assembly lighting system

This invention relates to the field of inspection assemblies and in particular to inspection assemblies that include light sources for illuminating a field of view of a camera. An inspection assembly for imaging the internal surface of a pipe or conduit comprises an elongate housing having a longitudinal axis, a camera mounted in the housing and arranged to capture an image of a region within a field of view external to the housing, a light source mounted in the housing and arranged to illuminate the field of view, and a window element mounted in the housing, the window element comprising a light transmitting material and being located such that light emitted by the light source passes through the window element before illuminating the field of view. The window element has an internal surface, closer to the light source, and an external surface, further from the light source, and the external surface comprises a concave region.

Thermophilic micro-organisms for ethanol production

Fremstilling af makrolidforbindelser

Processo para a preparacao de compostos macrolidos

Process for the preparation of macrolide compounds

Processo para a preparacao de compostos macrolidos

Enzymatic macrolide-conversion process

Process for producing macrolide compositions

Verfahren zur herstellung von macrolidderivaten

Die Erfindung betrifft ein Verfahren zur Herstellung von Makrolid-Derivaten. Erfindungsgemaesz werden Derivate von S541 (LL-F28249) der Formel (I) hergestellt, worin R1, R2 R3 und R4 die in der Beschreibung und in den Anspruechen angegebene Bedeutung aufweisen. Erfindungsgemaesz werden sie hergestellt durch Inkubieren einer entsprechenden 5-Ketoverbindung in einem geeigneten Medium in Gegenwart eines Mikroorganismus oder eines davon abgeleiteten Enzyms oder einer Praeparation, die von einem Mikroorganismus hergestellt wurde und das interessierende Enzym enthaelt, das in der Lage ist, die Umsetzung zu bewirken, hergestellt. Formel (I){neues Herstellungsverfahren; Antibiotika S541;, Wirkung gegen Endoparasiten; Ectoparasiten; Fungi; Insekten; Nematoden; Acariden}

Method of delivering nucleic acid to immune cells using rbcev

The invention relates to the use of red blood cells extracellular vesicle (RBCEV) to deliver nucleic acids to immune cells (e.g. T cells), wherein the nucleic acid cargoes are loaded into the RBCEV in vitro or ex vivo. The invention also relates to the prophylactic and therapeutic use of said immune cells in diseases, such as cancer.

Method of delivering nucleic acid to immune cells using rbcev

Downhole imaging

This invention relates to a method of imaging a downhole region of interest. In particular this invention relates to a method of processing images captured downhole to remove or reduce distortions due to the optical system as a function of the temperature experienced by the optical system in the downhole environment. A method of imaging a downhole region of interest comprises contemporaneously capturing an image of a region of interest and recording a temperature associated with the region of interest; selecting a pre-defined set of distortion data from a plurality of pre-defined sets of distortion data based on the recorded temperature; and applying the selected set of distortion data to the captured image to create an un-distorted image, wherein the pre-defined set of distortion data comprises distortion values associated with a range of field angles at a specified temperature.

Downhole imaging

This invention relates to a method of imaging a downhole region of interest. In particular this invention relates to a method of processing images captured downhole to remove or reduce distortions due to the optical system as a function of the temperature experienced by the optical system in the downhole environment. A method of imaging a downhole region of interest comprises contemporaneously capturing an image of a region of interest and recording a temperature associated with the region of interest; selecting a pre-defined set of distortion data from a plurality of pre-defined sets of distortion data based on the recorded temperature; and applying the selected set of distortion data to the captured image to create an un-distorted image, wherein the pre-defined set of distortion data comprises distortion values associated with a range of field angles at a specified temperature.

Downhole imaging

A method of processing images captured downhole to remove or reduce distortions caused by the temperature experienced by the optical system in the downhole environment. The method comprises contemporaneously capturing an image downhole and recording a temperature associated with the image, selecting a pre-defined set of distortion data from a plurality of pre-defined sets of distortion data based on the recorded temperature, and applying the selected set of distortion data to the captured image to create an un-distorted image, wherein the pre-defined set of distortion data comprises distortion values associated with a range of field angles at a specified temperature. The data may also be calibrated for different wavelengths of light. The method corrects distortion caused by expansion and contraction of lens elements 44/48.

Process for the preparation of an indacene compound

Derivados ciclicos de cetal, procedimiento para su preparacion y composicion farmaceutica que los contiene.

Production of mersacidin and its variants in SigH and/or mrsA negative bacillus host cells

The present invention relates to methods and products for improved production of the lantibiotic mersacidin. There is provided a method of producing a mersacidin variant which comprises introducing into a cell which is a ΔMrsA host cell an expression vector encoding said variant, and recovering said variant from the cell culture. Preferred variants include the novel variants of Table 1, e.g. mersacidin F3W, said mersacidin F3W optionally having a further one or more variations, e.g. mersacidin F3W G8A. Also provided is a Bacillus cell which is a SigH deficient strain of Bacillus sp. HIL Y-85,54728 (NCIMB Accession Number NCIMB 41211); this cell may also be used in the production of mersacidin and its variants. Further aspects of the invention include a DNA cassette which comprises a nucleotide sequence encoding the mersacidin mrsA propeptide, and a method to transform a Bacillus HIL host cell with plasmid, which method includes the step of electroporation.

Thermophilic microorganisms with inactivated lactate dehydrogenase gene (LDH) for ethanol production

Multi-stage equalisation method and apparatus for use in telemetry

The present invention relates to inspection apparatus for use in wellbores in the oil and gas industries. In particular the invention relates in general to the field of transmission of data between downhole module in a wellbore and a controlling module at the surface. The invention provides a method and apparatus for determining analog filter parameters for an analog front end comprising a plurality of filter stages receiving signals from a telemetry module, by repeating the steps of; receiving a signal of a known frequency and processing said signal by determining the magnitude of the frequency of the received signal until a plurality of signals have been received and processed; calculating an optimum set of filter parameters in dependence upon the measured frequency magnitudes and a predefined set of filter stage frequency responses.