Настройки

Укажите год
-

Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

Подробнее
-

Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

Подробнее

Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Укажите год
Укажите год
Применить Всего найдено 15. Отображено 10.
07-06-2012 дата публикации

Kits for assaying enzyme-mediated oxidative demethylation

Номер: US20120142038A1
Автор: Barbara Scheuer, Raymond Trievel, Russell Hart
Принадлежит: Arbor Assays LLC

Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent.

Подробнее
Номер записи: 1
22-11-2012 дата публикации

Fluorescent molecular probes for use in assays that measure test compound competitive binding with sam-utilizing proteins

Номер: US20120295283A1
Автор: Daniel Austin Bochar, Fred Lawrence Ciske, Gregory Scott Keyes, Gregory William ENDRES, Jeffrey Keith Johnson, Levi Lynn Blazer, Margaret Lynn Collins, Ranjinder Singh Sidhu, Raymond C. Trievel, Stephen Douglas Barrett
Принадлежит: Cayman Chemical Co Inc, University of Michigan

Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM-utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM-utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding.

Подробнее
Номер записи: 2
02-01-2014 дата публикации

Methods for assaying enzyme-mediated oxidative demethylation

Номер: US20140004550A1
Автор: Barbara Scheuer, Raymond Trievel, Russell Hart
Принадлежит: Arbor Assays LLC

Methods of screening a test agent for activity to modulate an oxidative demethylation enzyme are provided according to embodiments of the present invention which includes preparing a test reaction comprising an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme, a test agent and 4-amino-3-penten-2-one under conditions which allow generation of formaldehyde by oxidative demethylation of the substrate by the enzyme in the absence of the test agent; detecting fluorescence in the test reaction, the fluorescence indicative of reaction of formaldehyde and 4-amino-3-penten-2-one in the test reaction; and comparing fluorescence detected in the test reaction with a control, wherein a difference in detected fluorescence is indicative of a change in activity of the oxidative demethylation enzyme in the presence of the test agent.

Подробнее
Номер записи: 3
12-11-2015 дата публикации

Fluorescent molecular probes for use in assays that measure test compound competitive binding with sam-utilizing proteins

Номер: US20150322107A1
Автор: Daniel Austin Bochar, Fred Lawrence Ciske, Gregory Scott Keyes, Gregory William ENDRES, Jeffrey Keith Johnson, Levi Lynn Blazer, Margaret Lynn Collins, Ranjinder Singh Sidhu, Raymond C. Trievel, Stephen Douglas Barrett
Принадлежит: Cayman Chemical Co Inc, University of Michigan

Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM-utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM-utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding.

Подробнее
Номер записи: 4
12-11-2015 дата публикации

Fluorescent molecular probes for use in assays that measure test compound competitive binding with sam-utilizing proteins

Номер: US20150323542A1
Автор: Daniel Austin Bochar, Fred Lawrence Ciske, Gregory Scott Keyes, Gregory William ENDRES, Jeffrey Keith Johnson, Levi Lynn Blazer, Margaret Lynn Collins, Ranjinder Singh Sidhu, Raymond C. Trievel, Stephen Douglas Barrett
Принадлежит: Cayman Chemical Co Inc, University of Michigan

Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM-utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM-utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding.

Подробнее
Номер записи: 5
22-07-2010 дата публикации

Methods and compositions relating to enzyme assays

Номер: US20100184108A1
Автор: Barbara Scheuer, Raymond Trievel, Russell Hart
Принадлежит: Barbara Scheuer, Raymond Trievel, Russell Hart

Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent.

Подробнее
Номер записи: 6
17-02-2011 дата публикации

Methods and compositions relating to enzyme assays

Номер: WO2010083286A3
Автор: Barbara Scheuer, Raymond Trievel, Russell Hart
Принадлежит: Luminos, Llc

Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent.

Подробнее
Номер записи: 7
22-07-2010 дата публикации

Methods and compositions relating to enzyme assays

Номер: WO2010083286A2
Автор: Barbara Scheuer, Raymond Trievel, Russell Hart
Принадлежит: Luminos, Llc

Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent.

Подробнее
Номер записи: 8
01-07-2014 дата публикации

Method of screening a test agent for activity to modulate an oxidative demethylation enzyme

Номер: US8765396B2
Автор: Barbara Scheuer, Raymond Trievel, Russell Hart
Принадлежит: Arbor Assays LLC

Methods of screening a test agent for activity to modulate an oxidative demethylation enzyme are provided according to embodiments of the present invention which includes preparing a test reaction comprising an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme, a test agent and 4-amino-3-penten-2-one under conditions which allow generation of formaldehyde by oxidative demethylation of the substrate by the enzyme in the absence of the test agent; detecting fluorescence in the test reaction, the fluorescence indicative of reaction of formaldehyde and 4-amino-3-penten-2-one in the test reaction; and comparing fluorescence detected in the test reaction with a control, wherein a difference in detected fluorescence is indicative of a change in activity of the oxidative demethylation enzyme in the presence of the test agent.

Подробнее
Номер записи: 9
24-10-2013 дата публикации

Fluorescent molecular probes for use in assays that measure test compound competitive binding with sam-utilizing proteins

Номер: WO2012159072A4
Автор: Daniel Austin Bochar, Fred Lawrence Ciske, Gregory Scott Keyes, Gregory William ENDRES, Jeffrey Keith Johnson, Levi Lynn Blazer, Margaret Lynn Collins, Ranjinder Singh Sidhu, Raymond C. Trievel, Stephen Douglas Barrett
Принадлежит: Cayman Chemical Company, Incorporated, THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM-utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM- utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding.

Подробнее
Номер записи: 10