Antisense interference oligonucleotide used for inhibiting primer non-specific amplification

The invention relates to an antisense interference oligonucleotide used for inhibiting primer non-specific amplification. Through combining a primer 3' end antisense oligonucleotide, the antisense interference oligonucleotide interferes PCR primer 3' end non-specific amplification. The antisense oligonucleotide is a section of oligonucleotide with 4-14 base composed of antisense nucleotide. The basic characteristics are that the antisense base oligonucleotide maintains a Watson base pairing hybrid property, but loses the functions as PCR amplification template and primers. Antisense oligonucleotide is immobilized by using nanospheres, such that primers are indirectly immobilized to a PCR system, and hot start is sustained-released. Mineral oil is used for sealing PCR, such that aerosol cross-contamination is avoided, and PCR reaction is prevented from non-specific amplification. The antisense oligonucleotide is suitable for various PCR technologies with primer annealing and extension, including ...