B-1,3-glucanase, polynucleotide, recombinant vector, transformant, production method for B-1,3- glucanase, enzyme preparation, and production method for paramylon having reduced molecular weight

A β-1,3-glucanase that exhibits decomposition activity with respect to paramylon derived from the genus Euglena . The β-1,3-glucanase is derived from the genus Euglena and exhibits the properties indicated below: (1) effect: hydrolyzing the β-1,3-bond of β-1,3-glucan; and (2) substrate specificity: decomposing at least paramylon. The β-1,3-glucanase additionally exhibits the properties indicated below: (3) decomposition activity: the ratio of paramylon decomposition activity with respect to laminarin decomposition activity is 20% or higher; (4) optimum pH: 3.7-7.0; and (5) optimum temperature: 30-70° C.

Method for production of euglena containing wax ester at high content, and method for production of wax ester

Provided is a method for producing a euglena highly containing a wax ester, comprising aerobically culturing a microalga euglena by photosynthesis using carbon dioxide as a carbon source, then further culturing the microalga euglena under nitrogen-starvation conditions to increase paramylon accumulation per cell, and subsequently putting the microalga euglena under anaerobic conditions, so that a euglena highly containing a wax ester can be produced; and a method for producing a wax ester. The present invention relates to a method for producing a euglena highly containing a wax ester. The method comprises a first step of aerobically culturing a microalga euglena, a second step of further culturing a medium under nitrogen-starvation conditions, and a third step of retaining the cells under anaerobic conditions.

METHOD FOR PRODUCTION OF EUGLENA CONTAINING WAX ESTER AT HIGH COTENT, AND METHOD FOR PRODUCTION OF WAX ESTER

Provided is a method for producing a euglena highly containing a wax ester, comprising aerobically culturing a microalga euglena by photosynthesis using carbon dioxide as a carbon source, then further culturing the microalga euglena under nitrogen-starvation conditions to increase paramylon accumulation per cell, and subsequently putting the microalga euglena under anaerobic conditions, so that a euglena highly containing a wax ester can be produced; and a method for producing a wax ester. The present invention relates to a method for producing a euglena highly containing a wax ester. The method comprises a first step of aerobically culturing a microalga euglena, a second step of further culturing a medium under nitrogen-starvation conditions, and a third step of retaining the cells under anaerobic conditions.

PRODUCTION METHOD FOR BIOFUEL

The present invention provides a production method for biofuel based on a technology to convert carbon-dioxide as a carbon source through photosynthesis by photosynthetic microorganisms to biomass and produce biofuel of the biomass. The production method for biofuel of the present invention comprises a culturing process (S 1 ) of culturing in a culture solution photosynthetic microorganisms which store oils, fats and carbohydrates in cells of the photosynthetic microorganisms, an oil and fat conversion process (S 2 ) of converting the carbohydrates stored in the cells of the photosynthetic microorganisms cultured in the culture apparatus to oils and fats, an extraction process (S 3 ) of extracting the oils and fats out of the cells of the photosynthetic microorganisms, and a reforming process (S 4 ) to reform the extracted oils and fats.

Method for producing euglena having high wax ester content

Provided is a method that is for producing Euglena having a high wax ester content, capable of more efficiently producing the Euglena having a high wax ester content by adding a nutrient before anaerobic fermentation to restore the efficiency of wax ester fermentation. The present invention relates to a method for producing Euglena having a high wax ester content. The main configuration of the method is that there is at least a first step of aerobically culturing microalga Euglena under nitrogen starvation conditions and a second step of holding the cells in an anaerobic state; and adding, before the second step, a nutrient source to the culture liquid which has passed through the first step.

METHOD FOR PRODUCING FUEL OIL BASE

A method for producing a fuel oil base, comprising: a first step of aerobically culturing a microalga under a nitrogen-deficient condition; a second step of adding a nutrient to a treatment solution containing the microalga cultured in the first step, adjusting the dissolved oxygen concentration of the treatment solution to 0.03 mg/L or less, and performing anaerobic fermentation of the microalga to produce a wax ester; and a third step of hydrotreating a raw material oil containing the wax ester to produce a fuel oil base. 1. A method for producing a fuel oil base , comprising:{'i': 'Euglena', 'a first step of aerobically culturing a microalga under a nitrogen-deficient condition;'}{'i': Euglena', 'Euglena, 'a second step of adding a nutrient to a treatment solution containing the microalga cultured in the first step, adjusting a dissolved oxygen concentration of the treatment solution to 0.03 mg/L or less, and performing anaerobic fermentation of the microalga to produce a wax ester; and'}a third step of hydrotreating a raw material oil containing the wax ester to produce a fuel oil base.2. The production method according to claim 1 , wherein the second step is a step of adjusting the dissolved oxygen concentration of the treatment solution to 0.03 mg/L or less within 3 hours after the nutrient is added to the treatment solution.3. The production method according to claim 1 , wherein the nutrient contains a nitrogen source.4. The production method according to claim 3 , wherein the nitrogen source contains an ammonium compound.5. The production method according to claim 1 , wherein the nutrient contains a carbon source.6. The production method according to claim 5 , wherein the carbon source contains glucose.7. The production method according to claim 1 , wherein the third step includes a hydrorefining treatment and a hydroisomerization treatment as the hydrotreating.8. A fuel oil base produced by the production method according to .9. A method for producing a ...

B-1,3-glucanase, polynucleotide, recombinant vector, transformant, production method for b-1,3- glucanase, enzyme preparation, and production method for paramylon having reduced molecular weight

A β-1,3-glucanase that exhibits decomposition activity with respect to paramylon derived from the genus Euglena . The β-1,3-glucanase is derived from the genus Euglena and exhibits the properties indicated below: (1) effect: hydrolyzing the β-1,3-bond of β-1,3-glucan; and (2) substrate specificity: decomposing at least paramylon. The β-1,3-glucanase additionally exhibits the properties indicated below: (3) decomposition activity: the ratio of paramylon decomposition activity with respect to laminarin decomposition activity is 20% or higher; (4) optimum pH: 3.7-7.0; and (5) optimum temperature: 30-70° C.

Process for production of euglena containing wax ester at high content, and process for production of wax ester

[Problem] To provide: a process for producing euglena containing a wax ester at a high content, by culturing a microalga euglena aerobically by photosynthesis using carbon dioxide as a carbon source, further culturing the microalga euglena under nitrogen-starved conditions to increase the amount of accumulated paramylon per cell, and subsequently leaving the microalga euglena under anaerobic conditions, thereby producing euglena containing a wax ester at a high content; and a process for producing a wax ester. [Solution] The invention relates to a process for producing euglena containing a wax ester at a high content. The process comprises a first step of culturing a microalga euglena aerobically, a second step of further culturing the microalga euglena while retaining the culture medium under nitrogen-starved conditions, and a third step of retaining cells of the microalga euglena under anaerobic conditions.

Method for producing fuel oil base

A method for producing a fuel oil base, provided with: a first step for aerobically culturing microalgae Euglena under nitrogen-deficient conditions; a second step for adding a nutrient source to a solution to be treated, the solution containing the microalgae Euglena cultured in the first step, and then setting the dissolved oxygen concentration of the solution to be treated at 0.03 mg/L or lower, conducting anaerobic fermentation of the microalgae Euglena, and obtaining a wax ester; and a third step for hydrogenating stock oil containing the wax ester and obtaining a fuel oil base.

Process for production of euglena containing wax ester at high content, and process for production of wax ester

Provided is a method for producing a euglena highly containing a wax ester, comprising aerobically culturing a microalga euglena by photosynthesis using carbon dioxide as a carbon source, then further culturing the microalga euglena under nitrogen-starvation conditions to increase paramylon accumulation per cell, and subsequently putting the microalga euglena under anaerobic conditions, so that a euglena highly containing a wax ester can be produced; and a method for producing a wax ester. The present invention relates to a method for producing a euglena highly containing a wax ester. The method comprises a first step of aerobically culturing a microalga euglena, a second step of further culturing a medium under nitrogen-starvation conditions, and a third step of retaining the cells under anaerobic conditions.

Production method for biofuel

The present invention provides a production method for biofuel based on a technology to convert carbon-dioxide as a carbon source through photosynthesis by photosynthetic microorganisms to biomass and produce biofuel of the biomass. The production method for biofuel of the present invention comprises a culturing process (S 1 ) of culturing in a culture solution photosynthetic microorganisms which store oils, fats and carbohydrates in cells of the photosynthetic microorganisms, an oil and fat conversion process (S 2 ) of converting the carbohydrates stored in the cells of the photosynthetic microorganisms cultured in the culture apparatus to oils and fats, an extraction process (S 3 ) of extracting the oils and fats out of the cells of the photosynthetic microorganisms, and a reforming process (S 4 ) to reform the extracted oils and fats.

藻類含有組成物および藻類含有組成物製造システム

【課題】微細藻類を有効に利用することができる藻類含有組成物および藻類含有組成物製造システムを提供する。 【解決手段】キトサンと、微細藻類と、を含み、前記微細藻類が、バイオ燃料に変換可能な油脂を抽出された後の抽出残渣であって、食品、飼料、または肥料の添加剤として用いられることを特徴とする藻類含有組成物により上記課題を解決する。 【選択図】図１

ワックスエステル高含有ユーグレナの生産方法

【課題】嫌気発酵前に栄養素を添加してワックスエステルの発酵効率を回復させることによってワックスエステル高含有ユーグレナをより効果的に生産することが可能なワックスエステル高含有ユーグレナの生産方法を提供することにある。 【解決手段】ワックスエステル高含有ユーグレナの生産方法に関する。 微細藻ユーグレナを窒素欠乏条件下で好気的に培養する第１の工程と、細胞を嫌気状態下に保持する第２の工程と、を少なくとも行い、当該第２の工程を行う前に、第１の工程を経た培養液に栄養源を添加することを主要構成とする。 【選択図】図１

蠟酯含量高之綠蟲藻（Ｅｕｇｌｅｎａ）生產方法

本發明之課題在於提供一種蠟酯含量高之綠蟲藻之生產方法，其可藉由於厭氧醱酵前添加營養素使蠟酯之醱酵效率恢復而更有效地生產蠟酯含量高之綠蟲藻。本發明係關於一種蠟酯含量高之綠蟲藻之生產方法。本發明以如下內容作為主要構成：至少進行於缺氮條件下好氧培養微型藻綠蟲藻之第1步驟、及將細胞保持於厭氧狀態之第2步驟，並且於進行該第2步驟之前，將營養源添加至經過第1步驟之培養液。

Method for producing Euglena with high wax content