Use of a biopolymer for reducing the formation of a biofilm

The present invention relates to the use of a biopolymer for reducing the formation of a biofilm, preferably on a substrate, whereby the biopolymer is preferably a polypetide, such as recombinant spider silk polypeptide. The present invention further relates to methods for producing a substrate which reduces the formation of a biofilm on a surface of said substrate.

PRODUCTION OF RECOMBINANT COLLAGEN LIKE PROTEINS

The present invention is directed to a yeast cell for producing a recombinant collagen like protein. The present invention is further directed to a kit of parts or a co-expression system for use in the production of such a protein and to a method of producing said recombinant protein and a thread made therefrom. Furthermore, the invention pertains to proteins or threads obtainable by these methods as well as their use in various fields of technology and medicine.

Articles comprising a silk polypeptide for antigen delivery

The present invention relates to a polypeptide comprising a silk polypeptide and an antigen. Further, the present invention relates to an article comprising the polypeptide. Furthermore, the present invention relates to a pharmaceutical composition comprising the article. In addition, the present invention relates to the article or pharmaceutical composition for use as a pharmaceutical, for inducing an immune response and/or for use in a prophylactic and/or therapeutic treatment of a disease.

METHOD AND DEVICE FOR PRODUCING A THREAD FROM SILK PROTEINS

The invention relates to a method for producing a thread from silk proteins and to device for carrying out said method. The thus produced threads and the use thereof are also exposed. The invention uses a high-performance diffusion unity for producing high-quality silk threads.

PROCESS FOR THE PRODUCTION OF NANO AND MICRO CAPS FROM SPIDER SILK PROTEIN

Method of producing nano- and microcapsules of spider silk protein

The present invention is directed to a method of producing nano- and microcapsules from spider silk proteins The invention is further directed to nano- or microcapsules obtainable by this method as well as pharmaceutical, cosmetical and food compositions containing same.

Recombinant prion-like proteins and materials comprising same

The present invention provides novel polypeptides comprising a prion-aggregation domain and a second domain; novel polynucleotides encoding such polypeptides; host cells transformed or transfected with such polynucleotides; and methods of making and using the foregoing.

Modified spider silk proteins

Production of recombinant collagen like proteins

USE OF PROTEIN MICROBEADS IN COSMETICS

The invention relates to the use of protein microbeads in cosmetics.

MODIFIED SPIDER SILK PROTEINS

The present invention is directed to a method of modifying a spider silk protein and a spider silk protein obtainable by said method. The invention further pertains to a nucleic acid sequence coding for a modified spider silk protein, a vector containing said sequences and host cells transformed with this vector. The invention furthermore is directed to a pharmaceutical or cosmetical composition containing a modified spider silk protein as defined herein and the use of said modified sequences in various fields, in particular in the fields of medicine, cosmetics and technical applications. © KIPO & WIPO 2008 ...

Multilayer silk protein films

The present invention is directed to a method of forming multilayer silk protein films and a multilayer film obtained therefrom. The invention is further directed to various materials, products and compositions containing said multilayer film and to the use of Said multilayer film in several applications.

Multilayer silk protein films

The present invention is directed to a method of forming multilayer silk protein films and a multilayer film obtained therefrom. The invention is further directed to various materials, products and compositions containing said multilayer film and to the use of Said multilayer film in several applications.

Recombinant spider silk proteins

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

USE OF AMPHIPHILIC SELF-ASSEMBLING PROTEINS FOR FORMULATING POORLY WATER-SOLUBLE EFFECT SUBSTANCES

The invention relates to the use of amphiphilic self-assembling proteins for formulating poorly water-soluble effect substances.

ARTICLES COMPRISING A SILK POLYPEPTIDE FOR ANTIGEN DELIVERY

The present invention relates to a polypeptide comprising a silk polypeptide and an antigen. Further, the present invention relates to an article comprising the polypeptide. Furthermore, the present invention relates to a pharmaceutical composition comprising the article. In addition, the present invention relates to the article or pharmaceutical composition for use as a pharmaceutical, for inducing an immune response and/or for use in a prophylactic and/or therapeutic treatment of a disease.

MULTILAYER SILK PROTEIN FILMS

The present invention is directed to a method of forming multilayer silk protein films and a multilayer film obtained therefrom. The invention is further directed to various materials, products and compositions containing said multilayer film and to the use of Said multilayer film in several applications.

MULTILAYER SILK PROTEIN FILMS

The present invention is directed to a method of forming multilayer silk protein films and a multilayer film obtained therefrom. The invention is further directed to various materials, products and compositions containing said multilayer film and to the use of Said multilayer film in several applications.

Recombinant spider silk proteins

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

Recombinant spider silk proteins

USE OF AMPHIPHILIC SELF-ASSEMBLING PROTEINS FOR FORMULATING POORLY WATER-SOLUBLE EFFECT SUBSTANCES

The invention relates to the use of amphiphilic self-assembling proteins for formulating poorly water-soluble effect substances.

Recombinant prion-like genes and proteins and materials and methods comprising the same

The present invention provides novel polypeptides comprising a prion-aggregation domain and a second domain; novel polynucleotides encoding such polypeptides; host cells transformed or transfected with such polynucleotides; and methods of making and using the foregoing.

Multilayer silk protein films

Production of recombinant collagen like proteins

The present invention is directed to a yeast cell for producing a recombinant collagen like protein. The present invention is further directed to a kit of parts or a co-expression system for use in the production of such a protein and to a method of producing said recombinant protein and a thread made therefrom. Furthermore, the invention pertains to proteins or threads obtainable by these methods as well as their use in various fields of technology and medicine.

METHOD AND APPARATUS FOR THE PREPARATION OF A THREAD FROM SILK PROTEINS

The present invention relates to a thread preparation process from silk proteins including an apparatus which is appropriate for performing the method. Furthermore, the invention is directed to the threads obtained therewith as well as the use thereof. The invention uses a diffusion unit leading to the production of high-quality silk threads with high yield.

USE OF A BIOPOLYMER FOR REDUCING THE FORMATION OF A BIOFILM

The present invention relates to the use of a biopolymer for reducing the formation of a biofilm, preferably on a substrate, whereby the biopolymer is preferably a polypetide, such as recombinant spider silk polypeptide. The present invention further relates to methods for producing a substrate which reduces the formation of a biofilm on a surface of said substrate.

PRODUCTION OF RECOMBINANT COLLAGEN LIKE PROTEINS

The present invention is directed to a yeast cell for producing a recombinant collagen like protein. The present invention is further directed to a kit of parts or a co-expression system for use in the production of such a protein and to a method of producing said recombinant protein and a thread made therefrom. Furthermore, the invention pertains to proteins or threads obtainable by these methods as well as their use in various fields of technology and medicine.

Method of producing nano- and microcapsules of spider silk protein

The present invention is directed to a method of producing nano- and microcapsules from spider silk proteins The invention is further directed to nano- or microcapsules obtainable by this method as well as pharmaceutical, cosmetical and food compositions containing same.

Recombinant prion-like genes and proteins and materials and methods comprising same

Synthesis of spider dragline and/or flagelliform proteins

The present invention is directed to a method of producing spider dragline and/or flagelliform proteins. The invention is further directed to a method of producing spider threads and to a dragline/flagelliform protein or dragline/flagelliform protein thread produced by these methods. The invention further provides the use of these proteins/threads in the field of biotechnology and/or medicine, in particular in the manufacture of wound closure or coverage systems, suture materials and in the manufacture of replacement materials, preferably artificial cartilage or tendon materials.

Articles comprising a silk polypeptide for antigen delivery

The present invention relates to a polypeptide comprising a silk polypeptide and an antigen. Further, the present invention relates to an article comprising the polypeptide. Furthermore, the present invention relates to a pharmaceutical composition comprising the article. In addition, the present invention relates to the article or pharmaceutical composition for use as a pharmaceutical, for inducing an immune response and/or for use in a prophylactic and/or therapeutic treatment of a disease.

Verfahren und Vorrichtung zur Herstellung eines Fadens aus Seidenproteinen

Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung eines Fadens aus Seidenproteinen sowie eine Vorrichtung, die zur Durchführung des Verfahrens geeignet ist. Darüber hinaus ist die Erfindung auf daraus gewonnene Fäden sowie deren Verwendung gerichtet. Die Erfindung macht von einer Diffusionseinheit Gebrauch, die zur Erzeugung qualitativ hochwertiger Seidenfäden mit hoher Ausbeute führt.

Production of Recombinant Collagen Like Proteins

The present invention is directed to a yeast cell for producing a recombinant collagen like protein. The present invention is further directed to a kit of parts or a co-expression system for use in the production of such a protein and to a method of producing said recombinant protein and a thread made therefrom. Furthermore, the invention pertains to proteins or threads obtainable by these methods as well as their use in various fields of technology and medicine.

Use of a biopolymer for reducing the formation of a biofilm

The present invention relates to the use of a biopolymer for reducing the formation of a biofilm, preferably on a substrate, whereby the biopolymer is preferably a polypetide, such as recombinant spider silk polypeptide. The present invention further relates to methods for producing a substrate which reduces the formation of a biofilm on a surface of said substrate.

Method and device for producing a thread from silk proteins

The present invention relates to a thread preparation process from silk proteins including an apparatus which is appropriate for performing the method. Furthermore, the invention is directed to the threads obtained therewith as well as the use thereof. The invention uses a diffusion unit leading to the production of high-quality silk threads with high yield.

Method of producing nano- and microcapsules of spider silk protein

The present invention is directed to a method of producing nano- and microcapsules from spider silk proteins The invention is further directed to nano- or microcapsules obtainable by this method as well as pharmaceutical, cosmetical and food compositions containing same.

Methods of producing nano-and microcapsules of spider silk proteins

The present invention is directed to a method of producing nano- and microcapsules from spider silk proteins The invention is further directed to nano- or microcapsules obtainable by this method as well as pharmaceutical, cosmetical and food compositions containing same.

RECOMBINANT SPIDER SILK PROTEINS

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like. © KIPO & WIPO 2007 ...

Apparatus for the Preparation of a Thread From Silk Proteins

The present invention relates to a thread preparation process from silk proteins including an apparatus which is appropriate for performing the method. Furthermore, the invention is directed to the threads obtained therewith as well as the use thereof. The invention uses a diffusion unit leading to the production of high-quality silk threads with high yield.

METHOD OF PRODUCING NANO- AND MICROCAPSULES OF SPIDER SILK PROTEIN

Recombinant spider silk proteins

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

USE OF AMPHIPHILIC SELF-ASSEMBLING PROTEINS FOR FORMULATING POORLY WATER-SOLUBLE EFFECT SUBSTANCES

The invention relates to the use of amphiphilic self-assembling proteins for formulating poorly water-soluble effect substances.

Prozess zur Herstellung von Nano- und Mikrokapseln aus Spinnenseidenprotein

PRODUCTION OF RECOMBINANT COLLAGEN LIKE PROTEINS

The present invention is directed to a yeast cell for producing a recombinant collagen like protein. The present invention is further directed to a kit of parts or a co-expression system for use in the production of such a protein and to a method of producing said recombinant protein and a thread made therefrom. Furthermore, the invention pertains to proteins or threads obtainable by these methods as well as their use in various fields of technology and medicine. © KIPO & WIPO 2008 ...

MULTILAYER SILK PROTEIN FILMS

The present invention is directed to a method of forming multilayer silk protein films and a multilayer film obtained therefrom. The invention is further directed to various materials, products and compositions containing said multilayer film and to the use of Said multilayer film in several applications. © KIPO & WIPO 2009 ...

Method and Device for Producing a Thread from Silk Proteins

The present invention relates to a thread preparation process from silk proteins including an apparatus which is appropriate for performing the method. Furthermore, the invention is directed to the threads obtained therewith as well as the use thereof. The invention uses a diffusion unit leading to the production of high-quality silk threads with high yield.

Silk particles for controlled and sustained delivery of compounds

The present invention relates to a method of producing and loading silk particles, preferably spider silk particles, with a compound. In particular, the present invention provides a novel two step method for loading silk particles, preferably spider silk particles, with small and water-soluble compounds. Also disclosed are silk particles, preferably spider silk particles, loaded with at least one compound which are eminently suited as carriers for controlled and sustained delivery applications. Furthermore, the invention relates to pharmaceutical or cosmetic compositions comprising said silk particles, preferably spider silk particles, and a pharmaceutically active compound or cosmetic compound for controlled and sustained release. The present invention is also directed to silk particles, preferably spider silk particles, loaded with a compound obtainable by the method according to the invention.

Prozess zur Herstellung von Nano- und Mikrokapseln aus Spinnenseidenprotein

Use of protein microbeads in cosmetics

The invention relates to the use of amphiphilic self-assembling proteins for formulating poorly water-soluble effect substances.

METHOD AND DEVICE FOR PRODUCING A THREAD FROM SILK PROTEINS

The invention relates to a method for producing a thread from silk proteins and to device for carrying out said method. The thus produced threads and the use thereof are also exposed. The invention uses a high-performance diffusion unity for producing high-quality silk threads. © KIPO & WIPO 2008 ...

Microfluidic Device for Controlled Aggregation of Spider Silk

The present invention is directed to a device and method of controlling the phase separation of one or a mixture of two or more spider silk proteins, leading to the defined and controllable assembly of the said silk protein(s) to defined morphologies, such as spheres, nano fibrils, threads, etc.

USE OF BIPOLYMER FOR REDUCING THE FORMATION OF A BIOFILM

The present invention relates to the use of a biopolymer for reducing the formation of a biofilm, preferably on a substrate, whereby the biopolymer is preferably a polypeptide, such as recombinant spider silk polypeptide. The present invention further relates to methods for producing a substrate which reduces the formation of a biofilm on a surface of said substrate.

USE OF PROTEIN MICROBEADS IN COSMETICS

Use of protein microbeads in cosmetics ...

Modified spider silk proteins

The present invention is directed to a method of modifying a spider silk protein and a spider silk protein obtainable by said method. The invention further pertains to a nucleic acid sequence coding for a modified spider silk protein, a vector containing said sequences and host cells transformed with this vector. The invention furthermore is directed to a pharmaceutical or cosmetical composition containing a modified spider silk protein as defined herein and the use of said modified sequences in various fields, in particular in the fields of medicine, cosmetics and technical applications.

Biopolymer having excellent tensile strength, extensibility and toughness

The present invention relates to a biopolymer having excellent tensile strength, extensibility and toughness. The present invention further relates to a nucleic acid molecule encoding the biopolymer of the invention, a vector comprising the nucleic acid molecule of the invention and a non-human host transformed with the vector of the invention. Furthermore, the present invention relates to methods for producing a biopolymer of the invention and to foams, films, gels, coatings, particles, capsules, springs or nonwoven mats as well as fibers comprising or consisting of the biopolymer of the invention. The present invention also relates to a method of producing fibers and the biopolymer of the invention for use in medicine, agriculture, cosmetics as well as surface, paper, leather and/or textile processing.

RECOMBINANT SPIDER SILK PROTEINS

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

Synthesis of spider dragline and/or flagelliform proteins

The present invention is directed to a method of producing spider dragline and/or flagelliform proteins. The invention is further directed to a method of producing spider threads and to a dragline/flagelliform protein or dragline/flagelliform protein thread produced by these methods. The invention further provides the use of these proteins/threads in the field of biotechnology and/or medicine, in particular in the manufacture of wound closure or coverage systems, suture materials and in the manufacture of replacement materials, preferably artificial cartilage or tendon materials.

Multilayer Silk Protein Films

The present invention is directed to a method of forming multilayer silk protein films and a multilayer film obtained therefrom. The invention is further directed to various materials, products and compositions containing said multilayer film and to the use of Said multilayer film in several applications.

Method and device for producing a thread from silk proteins

The invention relates to a method for producing a thread from silk proteins and to device for carrying out said method. The thus produced threads and the use thereof are also exposed. The invention uses a high-performance diffusion unity for producing high-quality silk threads.

Proteins of natural origin and materials made therefrom

The present invention is directed to proteins of natural origin and materials made therefrom, in particular to threads, fibers, foams and gels derived therefrom. The invention further provides the use of these proteins/threads and materials in the field of technology, biotechnology and/or medicine, in particular in the manufacture of wound closure or coverage systems, suture materials and in the manufacture of replacement materials, preferably artificial cartilage or tendon materials, as well as in other commercial applications.

Use of amphiphilic self-assembling proteins for formulating poorly water-soluble effect substances

The invention relates to the use of amphiphilic self-assembling proteins for formulating poorly water-soluble effect substances.

Articles comprising a silk polypeptide for antigen delivery

The present invention relates to a polypeptide comprising a silk polypeptide and an antigen. Further, the present invention relates to an article comprising the polypeptide. Furthermore, the present invention relates to a pharmaceutical composition comprising the article. In addition, the present invention relates to the article or pharmaceutical composition for use as a pharmaceutical, for inducing an immune response and/or for use in a prophylactic and/or therapeutic treatment of a disease.

Recombinant spider silk proteins

Use of amphiphilic self-assembling proteins for formulating poorly water-soluble effect substances

The invention relates to the use of amphiphilic self-assembling proteins for formulating poorly water-soluble effect substances.

Recombinant spider silk proteins

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

Proteins of natural origin and materials made therefrom

The present invention is directed to proteins of natural origin and materials made therefrom, in particular to threads, fibers, foams and gels derived therefrom. The invention further provides the use of these proteins/threads and materials in the field of technology, biotechnology and/or medicine, in particular in the manufacture of wound closure or coverage systems, suture materials and in the manufacture of replacement materials, preferably artificial cartilage or tendon materials, as well as in other commercial applications.

Production of recombinant collagen like proteins

The present invention is directed to a yeast cell for producing a recombinant collagen like protein. The present invention is further directed to a kit of parts or a co-expression system for use in the production of such a protein and to a method of producing said recombinant protein and a thread made therefrom. Furthermore, the invention pertains to proteins or threads obtainable by these methods as well as their use in various fields of technology and medicine.

Methods for Producing High Toughness Silk Fibres

The present invention provides methods for producing a silk protein spinning dope solution suitable for producing high toughness fibres, the thus produced silk protein spinning dope solution, methods for producing fibres using said silk protein spinning dope solution.

ARTICLES COMPRISING A SILK POLYPEPTIDE FOR ANTIGEN DELIVERY

The present invention relates to a polypeptide comprising a silk polypeptide and an antigen. Further, the present invention relates to an article comprising the polypeptide. Furthermore, the present invention relates to a pharmaceutical composition comprising the article. In addition, the present invention relates to the article or pharmaceutical composition for use as a pharmaceutical, for inducing an immune response and/or for use in a prophylactic and/or therapeutic treatment of a disease. 1. A polypeptide comprising(i) a silk polypeptide and(ii) an antigen.2. The polypeptide of claim 1 , wherein the polypeptide further comprises an enzymatically cleavable linker.3. The polypeptide of claim 2 , wherein the antigen is connected to the silk polypeptide via the enzymatically cleavable linker.4. The polypeptide of or claim 2 , wherein the enzymatically cleavable linker is a protease cleavable linker.5. The polypeptide of claim 4 , wherein the protease cleavable linker is a cathepsin cleavable linker claim 4 , preferably a cathepsin S cleavable linker or a cathepsin B cleavable linker.6. A nucleic acid molecule encoding the polypeptide of any one of to .7. A method for producing a polypeptide comprising the step of:{'claim-ref': {'@idref': 'CLM-00006', 'claim 6'}, '(a) expressing the nucleic acid molecule of in a cell, thereby producing the polypeptide in the cell.'}8. An article comprising the polypeptide of any one of to .9. A method for producing an article comprising the steps of:{'claim-ref': [{'@idref': 'CLM-00001', 'claims 1'}, {'@idref': 'CLM-00005', '5'}], '(a) providing an aqueous solution comprising the polypeptide of any one of to , and'}(b) forming an article out of/from the solution provided in (a).10. A pharmaceutical composition comprising the article of .11. The pharmaceutical composition of claim 10 , wherein the composition further comprises an adjuvant.12. The pharmaceutical composition of claim 10 , wherein the composition does not further ...

Methods of producing nano-and microcapsules of spider silk proteins

The present invention is directed to a method of producing nano- and microcapsules from spider silk proteins The invention is further directed to nano- or microcapsules obtainable by this method as well as pharmaceutical, cosmetical and food compositions containing same.

Electrical conductors and devices from prion-like proteins

The present invention provides novel polypeptides comprising a prion-aggregation domain and a second domain; novel polynucleotides encoding such polypeptides; host cells transformed or transfected with such polynucleotides; novel fibrils with specific functionalities and unusually high chemical and thermal stability; and methods of making and using the foregoing.

Use of bipolymer for reducing the formation of a biofilm

The present invention relates to the use of a biopolymer for reducing the formation of a biofilm, preferably on a substrate, whereby the biopolymer is preferably a polypeptide, such as recombinant spider silk polypeptide. The present invention further relates to methods for producing a substrate which reduces the formation of a biofilm on a surface of said substrate.

Recombinant spider silk proteins

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

Microfluidic device for controlled aggregation of spider silk

The present invention is directed to a device and method of controlling the phase separation of one or a mixture of two or more spider silk proteins, leading to the defined and controllable assembly of the said silk protein(s) to defined morphologies, such as spheres, nano fibrils, threads, etc.

Multilayer silk protein films

The present invention is directed to a method of forming multilayer silk protein films and a multilayer film obtained therefrom. The invention is further directed to various materials, products and compositions containing said multilayer film and to the use of Said multilayer film in several applications.

Electrical Conductors and Devices from Prion-Like Proteins

The present invention provides novel polypeptides comprising a prion-aggregation domain and a second domain; novel polynucleotides encoding such polypeptides; host cells transformed or transfected with such polynucleotides; novel fibrils with specific functionalities and unusually high chemical and thermal stability; and methods of making and using the foregoing.

Proteins of natural origin and materials made therefrom

SILK PARTICLES FOR CONTROLLED AND SUSTAINED DELIVERY OF COMPOUNDS

The present invention relates to a method of producing and loading silk particles, preferably spider silk particles, with a compound. In particular, the present invention provides a novel two step method for loading silk particles, preferably spider silk particles, with small and water-soluble compounds. Also disclosed are silk particles, preferably spider silk particles, loaded with at least one compound which are eminently suited as carriers for controlled and sustained delivery applications. Furthermore, the invention relates to pharmaceutical or cosmetic compositions comprising said silk particles, preferably spider silk particles, and a pharmaceutically active compound or cosmetic compound for controlled and sustained release. The present invention is also directed to silk particles, preferably spider silk particles, loaded with a compound obtainable by the method according to the invention. 141.-. (canceled)42. A method of producing spider silk particles loaded with a compound comprising the steps of:i) providing spider silk particles comprising one or more spider silk polypeptides comprising at least two identical repetitive units, andii) incubating said spider silk particles with at least one compound, wherein the compound is water-soluble and has a molecular weight of between 50 Da and 20 kDa.43. The method of claim 42 , wherein the spider silk particles provided in step i) are produced by the steps of:a) providing an aqueous solution comprising one or more spider silk polypeptides comprising at least two identical repetitive units,b) triggering aggregation of the spider silk polypeptides to form spider silk particles, andc) separating the spider silk particles by phase separation.44. The method of claim 42 , wherein the compound is able to permeate into the matrix of the spider silk particles.45. The method of claim 42 , wherein at least 40% claim 42 , preferably at least 50% claim 42 , 60% claim 42 , 70% claim 42 , 80% claim 42 , 90% claim 42 , or 95% of ...

Method of producing nano- and microcapsules of spider silk protein

The present invention is directed to a method of producing nano- and microcapsules from spider silk proteins The invention is further directed to nano- or microcapsules obtainable by this method as well as pharmaceutical, cosmetical and food compositions containing same.

NOVEL BIOPOLYMER HAVING EXCELLENT TENSILE STRENGTH, EXTENSIBILITY AND TOUGHNESS

The present invention relates to a biopolymer having excellent tensile strength, extensibility and toughness. The present invention further relates to a nucleic acid molecule encoding the biopolymer of the invention, a vector comprising the nucleic acid molecule of the invention and a non-human host transformed with the vector of the invention. Furthermore, the present invention relates to methods for producing a biopolymer of the invention and to foams, films, gels, coatings, particles, capsules, springs or nonwoven mats as well as fibres comprising or consisting of the biopolymer of the invention. The present invention also relates to a method of producing fibres and the biopolymer of the invention for use in medicine, agriculture, cosmetics as well as surface, paper, leather and/or textile processing. 2. The biopolymer of claim 1 , wherein the amino acid sequence of formula I is selected from the group consisting of:(i) the amino acid sequence of SEQ ID NO: 1;(ii) an amino acid sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 1;(iii) an amino acid sequence encoded by a nucleic acid molecule of SEQ ID NO: 2;(iv) an amino acid sequence encoded by a nucleic acid sequence that hybridizes under stringent conditions to the complementary strand of the nucleic acid sequence of SEQ ID NO: 2; and(v) an amino acid sequence encoded by a nucleic acid sequence that is degenerate with respect to the nucleic acid sequence of (iii) or (iv); and(vi) a variant of the amino acid sequence of (i), wherein the variant differs from the amino acid sequence of (i) in 1 to 10 amino acids, wherein the variations are selected from the group consisting of substitutions, deletions, insertions and/or additions and wherein preferably the variations do not or do not substantially reduce the biophysical properties of the biopolymer formed with this repeat sequence as compared to a biopolymer formed with a repeat sequence comprising or consisting of the amino ...

USE OF BIPOLYMER FOR REDUCING THE FORMATION OF A BIOFILM

The present invention relates to the use of a biopolymer for reducing the formation of a biofilm, preferably on a substrate, whereby the biopolymer is preferably a polypeptide, such as recombinant spider silk polypeptide. The present invention further relates to methods for producing a substrate which reduces the formation of a biofilm on a surface of said substrate. 1. A method for reducing the formation of a biofilm on a substrate , comprising the step of:applying a biopolymer on the surface of a substrate or producing a substrate comprising a biopolymer, thereby reducing the formation of a biofilm on the substrate.2. (canceled)3. The method of claim 1 , wherein the biofilm is an aggregate of microorganisms in which cells adhere to each other and/or to the surface of the substrate.4. The method of claim 3 , wherein the microorganisms are selected from the group consisting of bacteria claim 3 , fungi claim 3 , algae claim 3 , and protozoa claim 3 , or any combination thereof.5. The method of claim 1 , wherein the biopolymer is applied in the form of a coating.6. The method of claim 1 , wherein the substrate is a device.7. The method of claim 6 , wherein the device is selected from the group consisting of a medical device claim 6 , a wastewater treatment device claim 6 , a heating device claim 6 , a ventilation device claim 6 , and an air condition device.8. The method of claim 1 , wherein the substrate is selected from the group consisting of a synthetic inert substrate claim 1 , an inorganic inert substrate claim 1 , and a naturally occurring substrate.9. The method of claim 8 , wherein the(i) synthetic inert substrate is selected from the group consisting of polyester, polystyrene, polyamide (PA), polyaramid, polytetrafluorethylene (PTFE), polyethylene (PE), polypropylene (PP), polyurethane (PU), silicone, a mixture of polyurethane and polyethylenglycol (elastane), ultra high molecular weight polyethylene (UHMWPE), and high-performance polyethylene (HPPE),(ii) ...

ARTICLES COMPRISING A SILK POLYPEPTIDE FOR ANTIGEN DELIVERY

The present invention relates to a polypeptide comprising a silk polypeptide and an antigen. Further, the present invention relates to an article comprising the polypeptide. Furthermore, the present invention relates to a pharmaceutical composition comprising the article. In addition, the present invention relates to the article or pharmaceutical composition for use as a pharmaceutical, for inducing an immune response and/or for use in a prophylactic and/or therapeutic treatment of a disease. 115-. (canceled)16. A method for delivering an antigen to a cell comprising the steps of: (a) a silk polypeptide,', '(b) an antigen, and', '(c) an enzymatically cleavable linker,, 'providing a polypeptide comprising'}wherein the antigen is connected to the silk polypeptide via the enzymatically cleavable linker, and(ii) administering the polypeptide provided in (i) to the cell.17. The method of claim 16 , wherein the cell is a cell of/comprised in a subject.18. The method of claim 16 , wherein the enzymatically cleavable linker is a protease cleavable linker.198. The method of claim claim 16 , wherein the protease cleavable linker is a cathepsin cleavable linker.20. The method of claim 19 , wherein the cathepsin cleavable linker is a cathepsin S cleavable linker or a cathepsin B cleavable linker.21. The method of claim 16 , wherein the polypeptide is comprised in a pharmaceutical composition which is free of any adjuvant.22. The method of claim 16 , wherein the polypeptide is comprised in an article.23. The method of claim 22 , wherein the article is selected from the group consisting of a particle claim 22 , capsule claim 22 , fiber claim 22 , film claim 22 , granule claim 22 , gel claim 22 , fabric made of fibers claim 22 , and a rod or bundles thereof.24. The method of claim 16 , wherein the cell is an antigen presenting cell.25. The method of claim 24 , wherein the antigen presenting cell is a dendritic cell and/or a macrophage.26. A method for prophylactic or therapeutic ...

Methods for Producing High Toughness Silk Fibres

The present invention provides methods for producing a silk protein spinning dope solution suitable for producing high toughness fibres, the thus produced silk protein spinning dope solution, methods for producing fibres using said silk protein spinning dope solution. 1. Method for producing a silk protein spinning dope solution comprising the steps:(a) providing an aqueous solution comprising at least one silk protein and a protein denaturant or mixture of protein denaturants at a silk protein denaturing concentration, wherein the total concentration of the silk protein(s) in the solution is less than 20% w/v;(b) reducing the concentration of the protein denaturant by 8-fold to 14-fold;(c) reducing the concentration of the protein denaturant by 1.5 to 3-fold; and(d) producing the silk protein spinning dope solution by concentrating the silk protein(s) in the solution at least 1.5-fold in comparison to its(their) concentration in step (a) to a concentration of at least 10% w/v.2. The method of claim 1 , wherein the silk protein is a spider silk protein claim 1 , preferably a dragline or flagelliform protein of an orb-web spider (Araneidae); insect silk protein claim 1 , or mussel byssus silk protein or a variant thereof.3. (canceled)4. The method of claim 2 , wherein the silk protein or variant thereof has a molecular weight of at least 20 kDa and comprises at least two repetitive units each comprising at least one consensus sequence selected from the group consisting of:(a) GPGXX (SEQ ID NO: 3), wherein X is any amino acid, preferably in each case independently selected from the A, S, G, Y, P, and Q;(b) GGX, wherein X is any amino acid, preferably in each case independently selected from Y, P, R, S, A, T, N and Q; and{'sub': 'x', '(c) A, wherein x is an integer from 5 to 10.'}5. The method of claim 4 , wherein the repetitive units are independently selected from module A (SEQ ID NO: 20) claim 4 , module C (SEQ ID NO: 21) claim 4 , module Q (SEQ ID NO: 22) claim 4 , ...

Recombinant spider silk proteins

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

Use of amphiphilic self-assembling proteins for formulating poorly water-soluble effect substances

Die Erfindung betrifft dieVerwendung von amphiphilen selbstassemblierenden Proteinen zur Formulierung von schwer wasserlöslichen Effektstoffen.

Electrical Conductors and Devices from Prion-Like Proteins

The present invention provides novel polypeptides comprising a prion-aggregation domain and a second domain; novel polynucleotides encoding such polypeptides; host cells transformed or transfected with such polynucleotides; novel fibrils with specific functionalities and unusually high chemical and thermal stability; and methods of making and using the foregoing.

Recombinant spider silk proteins

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

Multilayer Silk Protein Films

The present invention is directed to a method of forming multilayer silk protein films and a multilayer film obtained therefrom. The invention is further directed to various materials, products and compositions containing said multilayer film and to the use of Said multilayer film in several applications.

Recombinant prion-like genes and proteins and materials and methods comprising same

The present invention provides polypeptides comprising a prion-aggregation domain and a second domain; polynucleotides encoding such polypeptides; host cells transformed or transfected with such polynucleotides; and methods of making and using the foregoing.

Method and device for producing a thread from silk proteins

The invention relates to a method for producing a thread from silk proteins and to device for carrying out said method. The thus produced threads and the use thereof are also exposed. The invention uses a high-performance diffusion unity for producing high-quality silk threads.

Articles comprising silk polypeptide for antigen delivery

【課題】免疫応答を誘導するための、並びに／又は疾患の予防的及び／もしくは治療的処置における使用のための物品または薬学的組成物を提供する。【解決手段】外側の表面を有する固体の内側のマトリックスからなる粒子であって、内側のマトリックス及び外側の表面の両方がポリペプチドを含み、ポリペプチドが、（ｉ）シルクポリペプチド、（ｉｉ）抗原性のペプチド又はポリペプチド、及び（ｉｉｉ）酵素切断可能なリンカーを含み、抗原性のペプチド又はポリペプチドが、シルクポリペプチドに酵素切断可能なリンカーを介してつながっている、粒子、および該粒子を含む薬学的組成物を提供する。【選択図】なし

Modified spider silk proteins

The present invention is directed to a method of modifying a spider silk protein and a spider silk protein obtainable by said method. The invention further pertains to a nucleic acid sequence coding for a modified spider silk protein, a vector containing said sequences and host cells transformed with this vector. The invention furthermore is directed to a pharmaceutical or cosmetical composition containing a modified spider silk protein as defined herein and the use of said modified sequences in various fields, in particular in the fields of medicine, cosmetics and technical applications.

Prozess zur herstellung von nano- und mikrokapseln aus spinnenseidenprotein

Sposób otrzymywania nano-i mikrokapsułek z białka pajęczego jedwabiu

Articles comprising a silk polypeptide for antigen delivery

The present invention relates to a polypeptide comprising a silk polypeptide and an antigen. Further, the present invention relates to an article comprising the polypeptide. Furthermore, the present invention relates to a pharmaceutical composition comprising the article. In addition, the present invention relates to the article or pharmaceutical composition for use as a pharmaceutical, for inducing an immune response and/or for use in a prophylactic and/or therapeutic treatment of a disease.

Method of producing nano- and microcapsules of spider silk protein

The present invention is directed to a method of producing nano- and microcapsules from spider silk proteins The invention is further directed to nano- or microcapsules obtainable by this method as well as pharmaceutical, cosmetical and food compositions containing same.

Use of a biopolymer for reducing the formation of a biofilm

The present invention relates to the use of a biopolymer for reducing the formation of a biofilm. The present invention further relates to methods for producing a substrate which reduces the formation of a biofilm on a surface of said substrate.

Use of a biopolymer for reducing the formation of a biofilm

The present invention relates to the use of a biopolymer for reducing the formation of a biofilm. The present invention further relates to methods for producing a substrate which reduces the formation of a biofilm on a surface of said substrate.

Spider silk particles for controlled and sustained delivery of compounds

The present invention relates to a method of producing and loading silk particles, preferably spider silk particles, with a compound. In particular, the present invention provides a novel two step method for loading silk particles, preferably spider silk particles, with small and water-soluble compounds. Also disclosed are silk particles, preferably spider silk particles, loaded with at least one compound which are eminently suited as carriers for controlled and sustained delivery applications. Furthermore, the invention relates to pharmaceutical or cosmetic compositions comprising said silk particles, preferably spider silk particles, and a pharmaceutically active compound or cosmetic compound for controlled and sustained release. The present invention is also directed to silk particles, preferably spider silk particles, loaded with a compound obtainable by the method according to the invention.

Methods for producing high toughness silk fibres

The present invention provides methods for producing a silk protein spinning dope solution suitable for producing high toughness fibres, the thus produced silk protein spinning dope solution, methods for producing fibres using said silk protein spinning dope solution.

Method for producing a collagen and silk polypeptide composite structure

The present invention relates to a method for producing a collagen and silk polypeptide composite structure. In addition, the present invention relates to a collagen and silk polypeptide composite structure and uses thereof.

Microfluidic device for controlled aggregation of spider silk

The present invention is directed to a device and method of controlling the phase separation of one or a mixture of two or more spider silk proteins, leading to the defined and controllable assembly of the said silk protein(s) to defined morphologies, such as spheres, nano fibrils, threads, etc.