17-10-2013 дата публикации
Номер: US20130274124A1
The present invention provides arrays comprising polypeptides associated with extracellular matrix that can be used to isolate, differentiate, or culture certain cell types including stem cells, cancer cells, and/or primary hepatocytes. The array comprises at least a pair of polypeptides that comprise a polypeptide associated with extracellular matrix or functional fragments thereof. The invention also provides for methods of diagnosing and/or prognosing a certain disease or disorder through contacting a cell sample from a patient with an array comprising at least a pair of polypeptides that comprise a polypeptide sequence associated with extracellular matrix or functional fragments thereof. 1. An array of polypeptides , the array comprising: a solid support and a plurality of adhesion sets , wherein each adhesion set comprises two or more different polypeptides comprising a polypeptide sequence associated with the extracellular matrix or a functional fragment thereof , and wherein the adhesion sets are attached to the solid support at an addressable location of the array.2. The array of claim 1 , wherein the solid support is a slide optionally coated with a polymer.3. The array of claim 1 , wherein the polymer is polyacrylamide.4. The array of claim 1 , wherein at least one adhesion set comprises two different polypeptides attached to a solid support.5. The array of claim 1 , wherein the two or more of the different polypeptides comprise at least 10 contiguous amino acids chosen from: collagen I claim 1 , collagen II claim 1 , collagen III claim 1 , collagen IV claim 1 , collagen V claim 1 , collagen VI claim 1 , fibronectin claim 1 , laminin claim 1 , merosin claim 1 , tenascin-R claim 1 , chondroitin sulfate claim 1 , agreccan claim 1 , elastin claim 1 , keratin claim 1 , mucin claim 1 , superfibronectin claim 1 , F-spondin claim 1 , nidogen-2 claim 1 , heparin sulfate claim 1 , biglycan claim 1 , decorin claim 1 , galectin 1 claim 1 , galectin 3 claim 1 , ...
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