20-02-2014 дата публикации
Номер: US20140051140A1
Принадлежит:
A method of biologically producing p-hydroxybenzoic acid, and a method for producing p-hydroxybenzoic acid from lignin through chemical and biological conversion. 1. A method for biologically producing p-hydroxybenzoic acid , comprising contacting a substrate with a biocatalyst , wherein the substrate comprises an aromatic carboxylic acid having (a) a p-hydroxy group and (b) a hydroxy group or C1-C4 alkoxy group attached to at least one other position of the aromatic ring , and the biocatalyst removes the hydroxy group or C1-C4 alkoxy group of (b) to produce p-hydroxybenzoic acid.3. The method of claim 1 , wherein the aromatic carboxylic acid is vanillic acid or syringic acid.4. The method of claim 1 , wherein the biocatalyst is an enzyme claim 1 , a microorganism claim 1 , a lysate of a microorganism claim 1 , or an extract of a microorganism lysate.5. The method of claim 4 , wherein the biocatalyst comprises anthranilate synthase claim 4 , 5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate-lyase claim 4 , chorismate lyase claim 4 , 3-dehydroshikimate hydro-lyase claim 4 , isochorismate lyase claim 4 , aminodeoxychorismate lyase claim 4 , 3-dehydroquinate hydro-lyase claim 4 , prephenate hydro-lyase claim 4 , or hydroxyphenylpyruvate synthase.6Saccharomyces, Zygosaccharomyces, Schizosaccharomyces, Kluyveromyces, Candida, Hansenula, Debaryomyces, Nadsonia, Lipomyces, Torulopsis, Kloekera, Pichia, Trigonopsis, Brettianomyces, Aspergillus, Yarrowia, Cryptococcus, Aureobasidium, Rhizopus, Monascus, Leucosporidium, Issatchenkia, Streptococcus, Escherichia, Bacillus, Brucella suis, Mycobacterium, Salmonella, Shigella, Yersinia, Aquifex, Helicobacter, Staphylococcus, Thermotoga, Pseudomonas, Sinorhizobium, Vibrio, Schizosaccharomyces, Clostridium, Lactobacillus, Klebsiella, Citrobacter,Streptomyces.. The method of claim 4 , wherein the enzyme is derived from or7. The method of claim 4 , wherein the microorganism is a wild type or a recombinant microorganism.8. The method ...
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