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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 3. Отображено 3.
28-05-2008 дата публикации

Site differential recombination enzyme, preparation method and application thereof

Номер: CN0101186906A
Автор: ZHU HUANZHANG, LIU SHAOHUI, MA JINFANG, XIN QINGTING, PENG SIMAN, TANG LISHA, YU LONG, HUANZHANG ZHU, SHAOHUI LIU, JINFANG MA, QINGTING XIN, SIMAN PENG, LISHA TANG, LONG YU
Принадлежит:

The invention belongs to the field of genetic engineering, in particular relating to a site-specific recombinase, process for preparation and application. The invention provides a novel site-specific recombinase of which the specificity identifies the below structural formula which indicates a dideoxynucleotide sequence, the dideoxynucleotide sequence is cracked in the position of arrows, and a sequence of attB recognition site and the sequence indicated by the structural formula are reconnected at the cleavage site (see figure). The site-specific recombinase of the invention is capable of specifically identifying a genome MpsL1 site with comparatively high activity, and thereby the site-specific recombinase of the invention can be an effective tool in technical operation of the genetic engineering, in particular to gene site-specific insertion recombination.

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Номер записи: 1
04-05-2011 дата публикации

Phi C31 site specific recombinase mutant, preparation method thereof and application

Номер: CN0101457216B
Автор: XIN QINGTING, PENG SIMAN, LIU SHAOHUI, TANG LISHA, YU LONG, ZHU HUANZHANG, MA JINFANG, QINGTING XIN, SIMAN PENG, SHAOHUI LIU, LISHA TANG, LONG YU, HUANZHANG ZHU, JINFANG MA
Принадлежит:

The invention belongs to the genetic engineering field, especially to a site-specific recombinase, a preparation method thereof and applications. A phi C31 site-specific recombinase is an effective tool for mammalian cells genetic operation, but its mediated integration site specificity and integration efficiency on mammalian cells await further improvement. Thus the invention provides a phi C13 site-specific recombinase mutant capable of identifying a genome MpsL1 site with specificity and having higher activity. Thereby the site-specific recombinase of the invention can be an effective toolfor the genetic engineering operation, especially the gene fixed-point insertion and recombination, and open up a novel path for researching the gene function analysis and the gene therapy.

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Номер записи: 2
17-06-2009 дата публикации

Phi C31 site specific recombinase mutant, preparation method thereof and application

Номер: CN0101457216A
Автор: ZHU HUANZHANG, XIN QINGTING, MA JINFANG, LIU SHAOHUI, TANG LISHA, PENG SIMAN, YU LONG, HUANZHANG ZHU, QINGTING XIN, JINFANG MA, SHAOHUI LIU, LISHA TANG, SIMAN PENG, LONG YU
Принадлежит:

The invention belongs to the genetic engineering field, especially to a site-specific recombinase, a preparation method thereof and applications. A phi C31 site-specific recombinase is an effective tool for mammalian cells genetic operation, but its mediated integration site specificity and integration efficiency on mammalian cells await further improvement. Thus the invention provides a phi C13 site-specific recombinase mutant capable of identifying a genome MpsL1 site with specificity and having higher activity. Thereby the site-specific recombinase of the invention can be an effective tool for the genetic engineering operation, especially the gene fixed-point insertion and recombination, and open up a novel path for researching the gene function analysis and the gene therapy.

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Номер записи: 3