14-05-2014 дата публикации
Номер: CN103789446A
Принадлежит:
The invention discloses a kit and a method for detecting polymorphism of a pyrosequencing clopidogrel personalized drug gene, and relates to clopidogrel. The kit comprises a whole blood genomic DNA extraction reagent, a multi-polymerase chain reaction (PCR) amplification primer, a multi-PCR amplification reaction reagent, a single-stranded DNA separation and purification reagent, a pyrosequencing primer, a pyrosequencing reagent and a kit body. The detection method comprises the steps of extracting whole blood genomic DNA; implementing multi-PCR amplification reaction; separating and purifying a single-stranded DNA sample; and carrying out pyrosequencing and result analysis. The kit, mainly through detecting principal factors, i.e., important gene polymorphic sites of CYP2C19, in particular CYP2C19*2 and CYP2C19*3, affecting clopidogrel metabolism to result in individual curative effect difference, can guide therapy for patient of cardiovascular and cerebrovascular diseases requiring long-term ...
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