Construction of microRNA gene-mediated novel tissue engineered nerve and applications thereof in repairing nerve defect

Applications of a microRNA gene based on one or more microRNA genes of miRNA, miR-21, or miR-222 of the let-7 family in tissue engineered nerve construction and in repairing a peripheral nerve defect. The outer and/or inner surfaces or the interior of pores of a tissue engineered nerve transplant can be covered or adsorbed with a macromolecular material nanosphere carrying an miRNA inhibitor, miR-21, or miR-222 of the let-7 family or a mimic thereof, the macromolecular material consisting of biocompatible fibronectin and heparin. By regulating the expression of the microRNA genes to promote peripheral nerve regeneration and tissue engineered nerve construction, in vitro primary cultured Schwann cell proliferation is effectively promoted and an anti-apoptotic effect is provided with respect to neurons; in vitro experiments show that the grafting of the tissue engineered nerve transplant favors peripheral nerve regeneration and is applicable in treating peripheral nerve damages.

CONSTRUCTION OF MICRORNA GENE-MEDIATED NOVEL TISSUE ENGINEERED NERVE AND APPLICATIONS THEREOF IN REPAIRING NERVE DEFECT

Applications of a microRNA gene based on one or more microRNA genes of miRNA, miR-21, or miR-222 of the let-7 family in tissue engineered nerve construction and in repairing a peripheral nerve defect. The outer and/or inner surfaces or the interior of pores of a tissue engineered nerve transplant can be covered or adsorbed with a macromolecular material nanosphere carrying an miRNA inhibitor, miR-21, or miR-222 of the let-7 family or a mimic thereof, the macromolecular material consisting of biocompatible fibronectin and heparin. By regulating the expression of the microRNA genes to promote peripheral nerve regeneration and tissue engineered nerve construction, in vitro primary cultured Schwann cell proliferation is effectively promoted and an anti-apoptotic effect is provided with respect to neurons; in vitro experiments show that the grafting of the tissue engineered nerve transplant favors peripheral nerve regeneration and is applicable in treating peripheral nerve damages.

Construction of microRNA gene-mediated novel tissue engineered nerve and applications thereof in repairing nerve defect

CONSTRUCTION OF MICRORNA GENE-MEDIATED NOVEL TISSUE ENGINEERED NERVE AND APPLICATIONS THEREOF IN REPAIRING NERVE DEFECT

Applications of a microRNA gene based on one or more microRNA genes of miRNA, miR-21, or miR-222 of the let-7 family in tissue engineered nerve construction and in repairing a peripheral nerve defect. The outer and/or inner surfaces or the interior of pores of a tissue engineered nerve transplant can be covered or adsorbed with a macromolecular material nanosphere carrying an miRNA inhibitor, miR-21, or miR-222 of the let-7 family or a mimic thereof, the macromolecular material consisting of biocompatible fibronectin and heparin. By regulating the expression of the microRNA genes to promote peripheral nerve regeneration and tissue engineered nerve construction, in vitro primary cultured Schwann cell proliferation is effectively promoted and an anti-apoptotic effect is provided with respect to neurons; in vitro experiments show that the grafting of the tissue engineered nerve transplant favors peripheral nerve regeneration and is applicable in treating peripheral nerve damages.

Construction of microRNA gene-mediated novel tissue engineered nerve and applications thereof in repairing nerve defect

Applications of a microRNA gene based on one or more microRNA genes of miRNA, miR-21, or miR-222 of the let-7 family in tissue engineered nerve construction and in repairing a peripheral nerve defect. The outer and/or inner surfaces or the interior of pores of a tissue engineered nerve transplant can be covered or adsorbed with a macromolecular material nanosphere carrying an miRNA inhibitor, miR-21, or miR-222 of the let-7 family or a mimic thereof, the macromolecular material consisting of biocompatible fibronectin and heparin. By regulating the expression of the microRNA genes to promote peripheral nerve regeneration and tissue engineered nerve construction, in vitro primary cultured Schwann cell proliferation is effectively promoted and an anti-apoptotic effect is provided with respect to neurons; in vitro experiments show that the grafting of the tissue engineered nerve transplant favors peripheral nerve regeneration and is applicable in treating peripheral nerve damages.

Construction of microrna gene-mediated novel tissue engineered nerve and applications thereof in repairing nerve defect

The present invention discloses use of one or more MicroRNA genes selected from miRNAs of Family Let-7, miR-21 or miR-222 in the construction of tissue engineered nerves and in the repair of peripheral nerve defects. An outer and/or internal surface or pores of a tissue engineered nerve graft are coated or adsorbed with polymeric nanomicrospheres carrying a Let-7 family miRNA inhibitor, miR-21, or miR-222, or a mimetic thereof, wherein the polymeric material is composed of biocompatible fibronectin and heparin. In the present invention, the regeneration of peripheral nerves and the construction of tissue engineered nerves are promoted by regulating the expression of MicroRNA genes which can effectively promote the proliferation of primary Schwann cells cultured in vitro and have an anti-apoptotic effect on neuronal cells. In-vivo test proves that bridging of the tissue engineered nerve graft can facilitate the regeneration of peripheral nerves, thus being useful in the treatment of peripheral ...

Method for preparing self-hardening spherical cement retarder by using bicrystal phosphogypsum

The invention relates to the technical field of comprehensive utilization of ardealite, and provides a method for preparing a self-hardening spherical cement retarder by using bicrystal ardealite, which comprises the following steps of: controlling a wet-process phosphoric acid process to produce bicrystal ardealite containing calcium sulfate dihydrate and calcium sulfate hemihydrate at the same time, washing the bicrystal ardealite, upgrading, pelletizing, and drying to obtain the self-hardening spherical cement retarder. And naturally hardening to obtain the spherical retarder. The green low-energy-consumption wet-process phosphoric acid process advocated in the industry and the gelling effect of calcium sulfate hemihydrate are fully combined, the spherical cement retarder low in impurity content and good in fluidity is obtained under the conditions that cement is not added, steam curing is not needed, and turning is not needed, and the method has remarkable significance on green functional ...

CONSTRUCTION OF MICRORNA GENE-MEDIATED NOVEL TISSUE ENGINEERED NERVE AND APPLICATIONS THEREOF IN REPAIRING NERVE DEFECT

Provided is a use of one or more MicroRNA genes selected from miRNAs of Family Let-7, miR-21 or miR-222 in the construction of tissue engineered nerves and in the repair of peripheral nerve defects. An outer and/or internal surface or pores of a tissue engineered nerve graft are coated or adsorbed with polymeric nanomicrospheres carrying a Let-7 family miRNA inhibitor, miR-21, or miR-222, or a mimetic thereof, wherein the polymeric material is composed of biocompatible fibronectin and heparin. The regeneration of peripheral nerves and the construction of tissue engineered nerves are promoted by regulating the expression of MicroRNA genes which can effectively promote the proliferation of primary Schwann cells cultured in vitro and have an anti-apoptotic effect on neuronal cells. In-vivo test proves that bridging of the tissue engineered nerve graft can facilitate the regeneration of peripheral nerves, thus being useful in the treatment of peripheral nerve injury. 1. A method for construction of tissue engineered nerves or repair of peripheral nerve defects , comprising regulating microRNAs (miRNAs) gene expression , wherein the miRNAs are one or more selected from Let-7 family miRNAs , miR-21 , or miR-222.2. The method according to claim 1 , wherein the regulating miRNAs gene expression comprising administering a Let-7 family miRNA inhibitor claim 1 , miR-21 claim 1 , miR-222 claim 1 , or a mimetic thereof to a tissue engineered nerve graft.3. The method according to claim 1 , wherein an outer and/or internal surface or pores of the tissue engineered nerve graft are coated or adsorbed with the Let-7 family miRNA inhibitor claim 1 , miR-21 claim 1 , miR-222 claim 1 , or a mimetic thereof.4. The method according to claim 3 , wherein the Let-7 family miRNA inhibitor claim 3 , miR-21 claim 3 , miR-222 or a mimetic thereof is present on the tissue engineered nerve graft material in an amount of 10 μg/g-10 mg/g.5. The method according to claim 1 , wherein the Let-7 family ...

Belt manure cleaning machine and chicken coop feeding facility

The utility model discloses a belt manure cleaning machine and chicken coop feeding facility, belt manure cleaning machine includes braced frame, braced frame is last to be equipped with at least one deck excrement cleaning mechanism, excrement cleaning mechanism includes drive roll, driven voller, clear excrement area, first pinch roller and second pinch roller, the drive roll the driven voller sets up respectively braced frame's both ends, clear excrement area parcel drive roll and driven voller, first pinch roller setting is in the inboard of drive roll sticis towards the top below in clear excrement area clear excrement area, the second pinch roller sets up the inboard of driven voller sticis towards the below top in clear excrement area clear excrement area. Belt manure cleaning machine and chicken coop feeding facility can effectively prevent the off tracking in excrement area clearly, and the equipment operation is stable.

Regeneration process of glove box waste molecular sieve for lithium battery liquid injection

The present invention relates to a regeneration process of a glove box waste molecular sieve for lithium battery liquid injection. The regeneration process comprises: a, treating a failure molecular sieve for 1-12 h at a temperature of 100-280 DEG C so as to remove water in the molecular sieve; b, heating to a temperature of 280-600 DEG C, and treating for 1-28 h so as to remove the water remained in the molecular sieve and the electrolyte organic solvent absorbed in the molecular sieve; and c, re-loading the treated molecular sieve in a glove box so as to be used. The present invention provides the process for recycling the glove box waste molecular sieve for lithium battery liquid injection.

Waste liquid and waste gas treatment system for sulfurized isobutylene production

The invention provides a waste liquid and waste gas treatment system for sulfurized isobutylene production. The system specifically comprises a waste liquid and waste gas feeding system; the incineration system is arranged behind the waste liquid and waste gas feeding system; the waste heat utilization system is arranged behind the incineration system; the flue gas quenching system is arranged behind the waste heat utilization system; the tail gas treatment system is arranged behind the flue gas quenching system; according to the waste liquid and waste gas treatment process, the two-stage incineration system, the waste heat boiler, the spraying quench tower, the dry-method deacidification tower, the bag-type dust collector and the two-section spraying absorption tower are adopted for two-time incineration through the incineration system; organic waste gas and organic waste liquid generated in the sulfurized isobutylene production process can be fully combusted to generate sulfur dioxide ...

Dung cleaner

The utility model discloses a dung cleaner, support with being used for including clear excrement area the support frame in clear excrement area, support frame's both ends are equipped with actuating mechanism respectively and change to the mechanism, actuating mechanism includes the drive roller, change and include driven changing to the roller to the mechanism, clear excrement area cover is established the drive roller with the driven outside that changes to the roller, clear excrement area is V type area. Dung cleaner, support frame can support excrement area clearly, and clear excrement area cover is established in the drive roller and the driven outside that changes to the roller, can actuating mechanism with change to the effect underdrive of mechanism with conveying excrement and urine, bring transport excrement and urine through the transport of adopting the V type, little, the high transmission efficiency of area can high -efficient conveying excrement and urine, has made things ...

Multifunctional treating and beautifying Chinese medicine acupoint plaster and its making process

The multifunctional treating and beautifying Chinese medicine acupoint plaster is prepared with bupleurum root, ledebouriella root, angelica, safflower, cinnabar and other seven kinds of Chinese medicinal materials as well as antioxidant preservative and water. The preparation process includes mixing, decoction, concentration to form ointment, spreading the ointment on medical adhesive plaster and cooling. It can eliminate chloasma and acne, whiten, beautify and reach other health functions via internal regulation.

Synchronous flotation and impurity removal process for ardealite

The invention belongs to the field of ardealite purification, and particularly relates to a synchronous flotation and impurity removal process for ardealite. According to the synchronous flotation impurity removal process, after phosphogypsum is re-pulped, a modified regulator, a foaming agent and a collecting agent are sequentially added, first-stage reverse flotation operation is carried out, rough concentrate and a foam product I are obtained, the obtained rough concentrate is mixed with the foaming agent and the collecting agent again, second-stage reverse flotation operation is carried out, and final concentrate and a foam product II are obtained; performing blank scavenging operation I on the foam product II to obtain a foam product III and middling I, returning the middling I to the first-stage reverse flotation operation, mixing the foam product I and the foam product III, adding a collecting agent to perform scavenging operation II to obtain tailings and middling II, and returning ...

Method for measuring internal resistance of electrode materials

The invention relates to a method for measuring the internal resistance of electrode materials. The method is characterized by including the following steps: (1) preparing the electrode materials and auxiliary materials to be measured, (2) placing the electrode materials and the auxiliary materials into a stirrer to be stirred to form slurry, (3) taking out the slurry to place the slurry into an oven to be dried, and manufacturing powder, (4) dividing the powder into a plurality of parts equal in amount, and pressing the powder into sheet materials on a tablet machine, (5) evenly coating the two sides of the sheet materials with silver powder, (6) placing the sheet materials coated with the silver powder into the oven, and drying organic solvents in the silver powder, (7) welding copper wires on the two sides of the dried sheet materials, (8) directly reading resistance values of the sheet materials through the copper wires with a resistance tester, and (9) calculating an average value, ...

Chinese medicine oral liquid for nourishing liver, invigorating kidney and beautifying

The Chinese medicine oral liquid is prepared white chrysanthemum, white tuchahoe, white hyacinth bean, giant typhonium tuber, angelica and other five kinds of Chinese medicinal materials as well as antioxidant preservative and water. The present invention has the health effects of regulating function of kidney, dispersing the stagnated liver energy, regulating vital energy, whitening skin, eliminating fatigue, strengthening immunity, beautifying, etc.

Construction of MicroRNA gene-mediated novel tissue engineered nerve and applications thereof in repairing nerve defect

Provided is a use of one or more MicroRNA genes selected from miRNAs of Family Let-7, miR-21 or miR-222 in the construction of tissue engineered nerves and in the repair of peripheral nerve defects. An outer and/or internal surface or pores of a tissue engineered nerve graft are coated or adsorbed with polymeric nanomicrospheres carrying a Let-7 family miRNA inhibitor, miR-21, or miR-222, or a mimetic thereof, wherein the polymeric material is composed of biocompatible fibronectin and heparin. The regeneration of peripheral nerves and the construction of tissue engineered nerves are promoted by regulating the expression of MicroRNA genes which can effectively promote the proliferation of primary Schwann cells cultured in vitro and have an anti-apoptotic effect on neuronal cells. In-vivo test proves that bridging of the tissue engineered nerve graft can facilitate the regeneration of peripheral nerves, thus being useful in the treatment of peripheral nerve injury.

Technology for repairing big-caliber concrete pipeline lining by utilizing thin wall stainless steel

The invention relates to a technology for repairing a big-caliber concrete pipeline lining by utilizing thin wall stainless steel. The repair technology comprises the following steps: (1) pretreating in the earlier stage; (2) rolling and transporting the thin wall stainless steel; (3) positioning, internally supporting and spot-welding the stainless steel lining; (4) welding a stainless steel lining pipe; (5) conducting liquid penetration testing on the weld joint of the stainless steel lining pipe; (6) pickling and inactivating the weld joint; (7) rounding off in the later period. The technology for repairing the big-caliber concrete pipeline lining by utilizing thin wall stainless steel provided by the invention adopts the thin wall stainless steel to repair the big-caliber concrete pipeline (the diameter of the pipeline is larger than DN2000); compared with the other pipeline repair schemes, the anticorrosion property of the repaired pipeline is greatly increased, the service life of ...

Forsythia suspensa and radix astragali compound traditional chinese medicine decoction-derived micro ribonucleic acid, and preparation method therefor and use thereof

The present disclosure discloses microRNAs derived from a Forsythiae Fructus-Astragali Radix compound traditional Chinese medicine decoction as well as a preparation method and a use thereof, wherein the microRNAs are selected from miRNAs (micro ribonucleic acid) with a nucleotide sequence as shown in SEQ ID NO. 1 to 15, preferably QQ_159, and comprises artificially synthesized QQ_159, plant QQ_159 and a precursor form of QQ_159 or a mature form of QQ_159; the nucleotide sequence of QQ _159 is as shown in SEQ ID NO. 14. The present disclosure discloses functional plant microRNAs or the extract containing the microRNAs extracted from the Forsythiae Fructus-Astragali Radix compound traditional Chinese medicine decoction, and proves through experimental testings that the microRNAs QQ_159 from the Forsythiae FructusAstragali Radix compound traditional Chinese medicine decoction has certain inhibiting effect and treating effect on viral influenza and pneumonia caused by novel coronavirus infection.

Bionic nerve graft and preparation method therefor

The present invention discloses a bionic nerve graft and a preparation method thereof. The bionic nerve graft includes a helical stent, a nanofiber membrane, guide fibers and hBMSC-ECM. A surface of the helical stent is coated with the nanofiber membrane, the guide fibers and the hBMSC-ECM are placed in a tube cavity of the helical stent, and the raw materials of the helical stent, the nanofiber membrane and the guide fibers are all chitosan. The preparation method includes the following steps: preparing the helical stent by a mold pouring method, coating the surface of the helical stent with the nanofiber membrane by an electrostatic spinning method, preparing the guide fibers by a template method, and filling the guide fibers and the hBMSC-ECM in the helical stent with the surface coated with the nanofiber membrane. The method provided by the method is simple and low in cost. The prepared bionic nerve graft ensures sufficient mechanical support, good compliance and guidance, provides a sufficient space for the transmission of nerve regeneration nutrients, provides microenvironment elements for nerve regeneration, and has high biocompatibility and low immunogenicity.

Application of bone marrow mesenchymal stem cell exosomes in treatment of parkinson's disease

The invention belongs to the field of biomedicine, and relates to an application of Bone Marrow Mesenchyml Stem Cell Exosomes (BMSC-Exos) in treating Parkinson's disease (PD), wherein the BMSC-Exos are generated by stimulating BMSCs with a culture solution and extracted from the culture solution after passage; and the culture solution of the BMSCs is an α-MEM culture solution containing FBS and PS. The BMSC-Exos can greatly improve a motor function of a model mouse with PD, protect dopaminergic neurons of the model mouse with PD, improve an olfactory function of the model mouse with PD, and also inhibit the activation of olfactory astrocytes of the model mouse with PD.

Anti-theft system based on computer artificial intelligence

Recombinant human collagen and construction method therefor

Cellular matrix nerve graft for repairing peripheral nerve injury and preparation method therefor

The invention discloses a cell matrix nerve graft for repairing peripheral nerve injury and a preparation method thereof. The cell matrix nerve graft comprises acellular matrix and a scaffold, wherein the acellular matrix is obtained by decellularization after secretion and formation of stem cells, and the acellular matrix is wrapped around the scaffold. The defects of autologous nerve transplantation repair can be overcome and the immunogenicity of allogeneic cell transplantation repair is avoided. The cell matrix nerve graft beneficial to nerve cell proliferation and migration is provided, a microenvironment beneficial to axon growth is established, the purposes of nerve regeneration and function repair are achieved, and a feasible solution is provided for clinical treatment of nerve defects.

Qualitative and semiquantitative detection kit for toxicants and qualitative and semiquantitative method for toxicants

Suspension containing low-methoxylation pectin and montmorillonite and used for treating acute diarrhea

Recombinant human collagen and construction method therefor

Compound preparation for neuranagenesis, and preparation method therefor and use thereof

Disclosed are a compound preparation for neuranagenesis, and a preparation method therefor and the use thereof. The compound preparation for neuranagenesis comprises the raw medicinal materials in parts by weight: 10-20 parts of raw Astragali radix, 10-20 parts of Rehmaimiae Radix praeparata, 10-20 parts of Actyranthes bidentata, 6-15 parts of Jujubae fructus, 6-15 parts of Lycii fructus, 6-15 parts of parched Ziziphi spinosae semen, 6-12 parts of Angelicae smensis radix, 3-9 parts of Carthami flos, 6-15 parts of Poria, 6-15 parts of parched Atractylodis macrocephalae rhizoma and 10-20 parts of Zaocys. The compound preparation can be used for preparing drugs to treat nerve damage diseases, and can be prepared into oral liquids, granules, dissolved granules and tablets.