Method for separating and detecting spontaneous mutation gene based on agarose gel denaturation and renaturation and biotin affinity adsorption

The invention relates to a method for separating and detecting a spontaneous mutation gene based on agarose gel denaturation and renaturation and biotin affinity adsorption, which belongs to the technical field of biology, and relates to a method for separating and detecting a mutation gene. The method comprises the following steps of: marking wild deoxyribonucleic acid (DNA) fragments by using biotin, mixing the biotin-marked wild DNA fragments and DNA fragments to be detected, and performing denaturation and renaturation, wherein at the moment, DNA fragments which contain mutation sites cannot form heteroduplex DNA molecules because the biotin-marked wild DNA fragments with the same migration rate as the DNA fragments are not hybridized with the DNA fragments, DNA fragments which do notcontain the mutation sites are hybridized with the corresponding biotin-marked wild DNA fragments to form the heteroduplex DNA molecules, and the heteroduplex DNA molecules carry biotin marks; and adsorbing ...

Method for separating and detecting spontaneous mutation gene based on agarose gel denaturation and renaturation and biotin affinity adsorption

Environment-friendly and energy-saving solid waste treatment device

The invention discloses an environment-friendly and energy-saving solid waste treatment device, which comprises a cleaning box, a hopper is fixedly installed at the top of the cleaning box, a water drainage pipe is fixedly installed below a filter screen and located on the lower wall face of the cleaning box, a water retaining mechanism is arranged on the outer side wall face of the cleaning box, an evaporation mechanism is fixedly installed on the outer side wall face of the cleaning box, the evaporation mechanism is located on the opposite side of the water retaining mechanism, and a water outlet pipe is arranged at the bottom of a drainage box. The invention relates to the technical field of solid waste treatment, the device is compact in structure, solid waste is fed into THE cleaning box from the hopper through a feeding mechanism composed of a third motor and a conveying belt with a feeding plate, a condensation pipe is cooled through sewage discharged from the drainage pipe, water ...

Single-component polymer cement based waterproof coating prepared on basis of redispersible latex powder and preparation method of waterproof coating

The invention discloses a single-component polymer cement based waterproof coating prepared on the basis of redispersible latex powder and a preparation method of the waterproof coating. The waterproof coating comprises the following components in parts by weight: 500-700 parts of redispersible latex powder, 2.8-3.3 parts of hydroxypropyl methyl cellulose, 280-320 parts of cement, 180-220 parts of heavy calcium carbonate powder, 180-220 parts of quartz powder and 280-320 parts of quartz sand. Solid and powdery redispersible latex powder and hydroxypropyl methyl cellulose are used, and liquid polymer latex is not used anymore, so that the precision of proportions of prepared materials is improved, the ratio of polymers to inorganic substances is strictly controlled, and the quality of the waterproof coating is ensured. The waterproof coating is convenient to use in construction, and can be used by only adding water according to a proportion in the field. The waterproof coating is convenient ...

Method for assisting in identifying weak winter wheat and special primer thereof

The invention discloses a method for assisting in identifying weak winter wheat. The genome DNA of wheat to be measured serves as a template, primers disclosed by a sequence 1 and a sequence 2 in a sequence table are performed with PCR amplification to obtain a first round of PCR amplification product; the first round of PCR amplification product serves as the template, primers disclosed by a sequence 3 and a sequence 4 in the sequence table are performed with PCR amplification to obtain a second round of PCR amplification product; the second round of PCR amplification product is performed with enzyme digestion by restricted incision enzyme BfaI; if the enzyme digestion product contains two strips, the wheat to be measured is weak winter wheat; and if the enzyme digestion product does not contain two strips, the wheat to be measured is candidate non weak winter wheat. The method for assisting in identifying weak winter wheat of the invention can judge the vernalization habit of wheat in ...

Method for constructing lichi core collection by using EST-SSR (Expresses Sequence Tag-Simple Sequence Repeat) molecular marker

The invention discloses a method for constructing lichi core collection, which comprises the following steps: selecting tender lichi leaves, and extracting genome DNAs according to varieties to obtain genome DNA samples of lichi; searching EST-SSR (Expresses Sequence Tag-Simple Sequence Repeat) of the lichi according to an EST sequence of the lichi, and designing an EST-SSR primer; carrying out PCR (Polymerase Chain Reaction) by using the EST-SSR primer to amplify the genome DNAs; carrying out electrophoretic analysis on PCR products to obtain existence data of alleles of the PCR products; and analyzing the obtained data to construct the core collection. In the method disclosed by the invention, the processes of EST-SSR-marked primer formation and experiment operation are simple and are easy to operate, an amplification band is clear, results are stable, and the method is suitable for analyzing genetic diversity among lichi resources, the core collection constructed on the basis almost ...

IRS configuration optimization and AoI scheduling optimization method in UAV-IRS assisted IoT network

The invention discloses an IRS configuration optimization and AoI scheduling optimization method in a UAV-IRS-assisted IoT network, the method is applied to an unmanned aerial vehicle with an IRS, the unmanned aerial vehicle corresponds to a plurality of IoT devices, and the method comprises the following steps: obtaining own current position information; determining an optimal moving direction according to the current position information and a Q table obtained by pre-training; moving to the next position information along the optimal moving direction; determining a reflection phase shift matrix of the IRS through a combinatorial optimization method according to the number of surface array elements of the IRS, the preset phase resolution and the signal amplitude of the IRS at the next position information; determining, at the next location information, a signal-to-noise ratio of each corresponding IoT device based on the reflective phase shift matrix; when the signal-to-noise ratio of ...

Detection jig for elevator brake body

An elevator brake body detection jig disclosed by the present invention comprises a bottom supporting plate, the top surface of the bottom supporting plate is provided with a sealing cover plate and a top limiting box, the sealing cover plate is connected with the top limiting box, the top surface of the bottom supporting plate is uniformly provided with fixing screw holes, and the top surface of the top limiting box is inlaid with a control panel. A control box body is embedded in the inner side wall of the top limiting box, a detection mechanism is arranged in the top limiting box and the sealing cover plate, the detection mechanism comprises a rotating roller rod, a brake ring body is arranged at one end of the rotating roller rod, the rotating roller rod is connected with the top limiting box through a fixed bearing, and the rotating roller rod is connected with the fixed plate body through a sleeving bearing. According to the elevator brake body detection jig adopting the structure ...

Sliding high voltage cable floating combination fixing device

The invention belongs to the field of electrical equipment, and relates to a sliding high voltage cable floating combination fixing device which comprises a lower plate component and an upper plate component connected to the surface of the upper portion of the lower plate component. A cable clamp is fixed on the top face of the upper plate component. The lower plate component comprises a bottom plate, a lower guide rail arranged on one side of the surface of the bottom plate, and a lower connection seat arranged on the other side of the surface of the bottom plate. The upper plate component comprises a top plate, an upper guide rail arranged on one side of the bottom face of the top plate, and an upper connection seat arranged on the other side of the bottom face of the top plate. The sliding high voltage cable floating combination fixing device is reliable in effect, when the sliding high voltage cable floating combination fixing device is used, cable moving can be limited in the vertical ...

Cable terminal test hydraulic support of GIS cable pressure gas chamber

The invention belongs to the field of the electrical equipment and relates to a cable terminal test hydraulic support of a GIS cable pressure gas chamber. The cable terminal test hydraulic support comprises a bottom beam; traveling wheels are mounted at the four corners of the bottom of the bottom beam; a pull rod is hinged to the front end of the upper surface of the bottom beam and a hand rail is arranged on the front end of the pull rod; a swing rod is connected to the bottom beam by use of a rotating shaft; a telescopic arm is connected to the front end portion of the swing rod and a sliding groove is formed in the telescopic arm; one end of a crank arm is slidingly connected to the sliding groove by use of a slider, while the other end of the crank arm is connected with the ratchet end of a ratchet rod; the lower end of the ratchet rod is hinged to the bottom beam; a fan-shaped angle regulating piece is hinged to the outer end of the telescopic arm; the top end of the fan-shaped angle ...

Shock absorption device of coal mine fully-mechanized excavating machine

The invention relates to a damping device of a coal mine fully-mechanized excavating machine, and belongs to the field of coal mining, by arranging a damping mechanism, when a connecting mechanism and the damping mechanism are installed on a main shaft of a cutting head, second springs in a plurality of cylinders can reduce the acting force between the cutting head and a fixing seat of the fully-mechanized excavating machine; the change of the distance between the two pistons can cause the change of the air pressure in the cylinder, when the two pistons are close to each other, air can enter the cylinder through the fine first air hole and the fine second air hole, the acting force between the cutting head and the roadheader fixing seat can be reduced in the process, and the shaking amplitude between the cutting head and the roadheader fixing seat is reduced. The damping mechanisms can play a role in damping, and can also be matched with the connecting mechanisms to be mounted on the two ...

Method for detecting single nucleotide polymorphism or small insertions and deletions by utilizing MutS proteins in genome range

The invention belongs to the field of biotechnology, and relates to a method for detecting single nucleotide polymorphism or small insertions and deletions by utilizing MutS proteins in a genome range, which comprises the following steps of: mixing a DNA molecule to be detected and a wild DNA molecule, performing modification and renaturation, and realizing specific hybridization of correspondingsegments of the DNA molecule to be detected and the wild DNA molecule; adding biotin-labeled MutS proteins, and recovering the proteins to complete the identification and extraction of mutational sites; and performing polymerase chain reaction (PCR) amplification and sequencing on the extracted substances to detect and analyze mutant genes. Compared with the conventional method, the method is more simple, convenient and sensitive, can accurately identify and detect the mutational sites, and is high in reliability.

Coal and wood dual purpose furnace for cooking and warming

The invention discloses a coal and wood dual purpose furnace for cooking and warming. The dual purpose furnace comprises a furnace body, a charging device, a fire grate, a furnace door and a cooking range, wherein the furnace body is provided with a fuel chamber and a combustion chamber, the fuel chamber is communicated with the combustion chamber, the fuel chamber is arranged above one side of the combustion chamber, the cooking range is arranged above the other side of the combustion chamber, the charging device is arranged on the furnace body and at the top of the fuel chamber, the fire grate is arranged in the combustion chamber, the lower part of the bottom of the fuel chamber is provided with a primary tuyere, and the upside of the fire grate is provided with a secondary tuyere. Thefuel chamber is arranged above one side of the combustion chamber and the cooking range is arranged above the other side of the combustion chamber so that the combustion chamber can not only heat thecooking ...

Pipeline shunting connecting device

The invention belongs to the technical field of pipeline shunting, discloses a pipeline shunting connecting device, and aims to solve the problems that when domestic water is used, shunting needs to be carried out on a pipeline, the pipeline needs to be connected with a shunting pipeline, water supply needs to be cut off at the moment, and then the domestic water of a corresponding resident is affected. In addition, residual water in the pipeline can run out during pipeline connection, so that water source waste is caused. According to the technical scheme, the pipeline drilling device comprises the two semi-arc-shaped arc plates, the arc plates comprise the upper arc plate and the lower arc plate, when the pipeline drilling device is installed, cutoff operation does not need to be conducted, when the pipeline drilling device is used, drilling operation is conducted on a pipeline under the relative sealing condition, and therefore leakage of a water source is reduced; the two pipelines ...

Machining equipment for metal powder preparation

The invention discloses machining equipment for metal powder preparation, and relates to the field of metal powder preparation.The machining equipment comprises a main support and a discharging tank, a control panel is fixedly installed on the side wall of the main support, the lower end of the discharging tank communicates with a powder preparation tank, and the lower end of the powder preparation tank communicates with a discharging tank; the drying structure is used for carrying out air drying treatment on the metal powder after powder discharging and comprises a gear ring rotationally installed on the outer side of the discharging tank, cotton cloth is fixedly installed on the lower surface of the gear ring, a fixing frame is fixedly installed at a lower end opening of the discharging tank, and a hollow ball is fixedly installed at the lower end of the fixing frame; according to the metal powder drying device, by arranging the drying structure, direct drying work after metal powder ...